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Characteristics and Function of the Chitin Binding Protein from Xenorhabdus nematophila.
Protein Pept Lett. 2019 Jul 04; 26(6):414-422.PP

Abstract

BACKGROUND

Genome sequence analysis (GenBank access No.: FN667742.1) shows that Xenorhabdus nematophila ATCC19061 contains one gene (Xn-cbp) encoding chitin binding protein (Xn-CBP).

OBJECTIVE

The present work aims to clarify the characteristics and function of Xn-CBP from X. nematophila HB310.

METHODS

In this study, the Xn-cbp gene was cloned and expressed in Escherichia coli BL21 (DE3). Substrate binding assays were performed to explain the ability of Xn-CBP combined with the polysaccharide. The insecticidal toxicity of Xn-CBP against the second-instar larvae of Helicoverpa armigera was determined by feeding method. Besides, the antifungal activity of Xn-CBP against Coniothyrium diplodiella, Verticillium dahlia, and Fusarium oxysporum was tested by spore germination assay and hyphal extension assay.

RESULTS

Xn-CBP encoded 199 amino acids with a calculated mass of 28 kDa, which contained a signal peptide and a chitin binding domain. The Bmax and Kd values of Xn-CBP to colloidal chitin were 2.46 and 4.08, respectively. Xn-CBP had insecticidal activity against the H. armigera with a growth inhibition rate of 84.08%. Xn-CBP had the highest spore germination inhibitory effect on C. diplodiella with the inhibition rate of 83.11%. The hyphal growth inhibition rate of Xn-CBP to F. oxysporum, 41.52%, was higher than the other two fungi.

CONCLUSION

The Xn-CBP had the highest binding ability to colloidal chitin and it showed insecticidal activity and antifungal activity. The present study laid a foundation for further exploitation and utilization of X. nematophila.

Authors+Show Affiliations

College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, China.College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, China.Luanping, State-owned Forestry Farm Management of Chengde City, Chengde 068250, China.College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, China.College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, China.College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, China.College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30919769

Citation

Liu, Jia, et al. "Characteristics and Function of the Chitin Binding Protein From Xenorhabdus Nematophila." Protein and Peptide Letters, vol. 26, no. 6, 2019, pp. 414-422.
Liu J, Song P, Zhang J, et al. Characteristics and Function of the Chitin Binding Protein from Xenorhabdus nematophila. Protein Pept Lett. 2019;26(6):414-422.
Liu, J., Song, P., Zhang, J., Nangong, Z., Liu, X., Gao, Y., & Wang, Q. (2019). Characteristics and Function of the Chitin Binding Protein from Xenorhabdus nematophila. Protein and Peptide Letters, 26(6), 414-422. https://doi.org/10.2174/0929866526666190327143335
Liu J, et al. Characteristics and Function of the Chitin Binding Protein From Xenorhabdus Nematophila. Protein Pept Lett. 2019 Jul 4;26(6):414-422. PubMed PMID: 30919769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristics and Function of the Chitin Binding Protein from Xenorhabdus nematophila. AU - Liu,Jia, AU - Song,Ping, AU - Zhang,Jie, AU - Nangong,Ziyan, AU - Liu,Xiaobei, AU - Gao,Yue, AU - Wang,Qinying, PY - 2018/11/14/received PY - 2019/03/07/revised PY - 2019/03/07/accepted PY - 2019/3/29/pubmed PY - 2019/8/23/medline PY - 2019/3/29/entrez KW - Antifungal activity KW - Xenorhabdus nematophila KW - chitin binding protein KW - insecticidal activity KW - spore germination KW - substrate binding. SP - 414 EP - 422 JF - Protein and peptide letters JO - Protein Pept Lett VL - 26 IS - 6 N2 - BACKGROUND: Genome sequence analysis (GenBank access No.: FN667742.1) shows that Xenorhabdus nematophila ATCC19061 contains one gene (Xn-cbp) encoding chitin binding protein (Xn-CBP). OBJECTIVE: The present work aims to clarify the characteristics and function of Xn-CBP from X. nematophila HB310. METHODS: In this study, the Xn-cbp gene was cloned and expressed in Escherichia coli BL21 (DE3). Substrate binding assays were performed to explain the ability of Xn-CBP combined with the polysaccharide. The insecticidal toxicity of Xn-CBP against the second-instar larvae of Helicoverpa armigera was determined by feeding method. Besides, the antifungal activity of Xn-CBP against Coniothyrium diplodiella, Verticillium dahlia, and Fusarium oxysporum was tested by spore germination assay and hyphal extension assay. RESULTS: Xn-CBP encoded 199 amino acids with a calculated mass of 28 kDa, which contained a signal peptide and a chitin binding domain. The Bmax and Kd values of Xn-CBP to colloidal chitin were 2.46 and 4.08, respectively. Xn-CBP had insecticidal activity against the H. armigera with a growth inhibition rate of 84.08%. Xn-CBP had the highest spore germination inhibitory effect on C. diplodiella with the inhibition rate of 83.11%. The hyphal growth inhibition rate of Xn-CBP to F. oxysporum, 41.52%, was higher than the other two fungi. CONCLUSION: The Xn-CBP had the highest binding ability to colloidal chitin and it showed insecticidal activity and antifungal activity. The present study laid a foundation for further exploitation and utilization of X. nematophila. SN - 1875-5305 UR - https://www.unboundmedicine.com/medline/citation/30919769/Characteristics_and_Function_of_the_Chitin_Binding_Protein_from_Xenorhabdus_nematophila_ L2 - https://www.ingentaconnect.com/openurl?genre=article&issn=0929-8665&volume=26&issue=6&spage=414&aulast=Liu DB - PRIME DP - Unbound Medicine ER -