Tags

Type your tag names separated by a space and hit enter

Analysis of Long Noncoding RNA Expression Profile in Human Pulmonary Microvascular Endothelial Cells Exposed to Lipopolysaccharide.
Cell Physiol Biochem 2019; 52(4):653-667CP

Abstract

BACKGROUND/AIMS

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are a continuum of life-threatening lung changes. Pulmonary vascular injury is one of the most important initial causes of ALI and ARDS. However, the functions of long noncoding RNAs (lncRNAs) in pulmonary endothelial injury remain largely unknown. The aim of the present study was to determine the lncRNA expression profile of human pulmonary microvascular endothelial cells (HPMECs) exposed to lipopolysaccharide (LPS) and explore the potential functions of differentially expressed lncRNAs.

METHODS

Microarray analysis was used to identify differentially expressed lncRNAs and mRNAs. Bioinformatics analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, lncRNA-mRNA coexpression network and transcription factor (TF)-lncRNA network analyses, were performed to predict the functions of significantly differentially expressed lncRNAs and mRNAs. Realtime polymerase chain reaction (PCR) was used to determine the expression of selected lncRNAs and mRNAs.

RESULTS

In this study, we found that 213 lncRNAs and 212 mRNAs were significantly differentially expressed in HPMECs exposed to LPS (fold change > 2.0, p < 0.05). Furthermore, we found that mRNAs co-expressed with lncRNAs were significantly enriched in the TNF signaling pathway, the NF-κB signaling pathway, cell adhesion molecules (CAMs), cytokine-cytokine receptor interactions, and extracellular matrix (ECM)-receptor interactions. The expression levels of all but one of the selected lncRNAs and mRNAs detected by real-time PCR were similar to those detected by microarray analysis.

CONCLUSION

Our data indicate that lncRNAs play an important role in LPS-induced pulmonary endothelial inflammation and barrier dysfunction and may be potential preventive and therapeutic targets for ALI and ARDS.

Authors+Show Affiliations

Department of Anesthesiology, Qianfoshan Hospital, Shandong University, Jinan, China.Department of Anesthesiology, Qianfoshan Hospital, Shandong University, Jinan, China.Department of Anesthesiology, Qianfoshan Hospital, Shandong University, Jinan, China.Department of Anesthesiology, Qianfoshan Hospital, Shandong University, Jinan, China.Department of Anesthesiology, Qianfoshan Hospital, Shandong University, Jinan, China.Department of Anesthesiology, Qianfoshan Hospital, Shandong University, Jinan, China, wyldgf@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30921505

Citation

Wang, Dong, et al. "Analysis of Long Noncoding RNA Expression Profile in Human Pulmonary Microvascular Endothelial Cells Exposed to Lipopolysaccharide." Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, vol. 52, no. 4, 2019, pp. 653-667.
Wang D, Gu C, Liu M, et al. Analysis of Long Noncoding RNA Expression Profile in Human Pulmonary Microvascular Endothelial Cells Exposed to Lipopolysaccharide. Cell Physiol Biochem. 2019;52(4):653-667.
Wang, D., Gu, C., Liu, M., Liu, G., Liu, H., & Wang, Y. (2019). Analysis of Long Noncoding RNA Expression Profile in Human Pulmonary Microvascular Endothelial Cells Exposed to Lipopolysaccharide. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 52(4), pp. 653-667. doi:10.33594/000000046.
Wang D, et al. Analysis of Long Noncoding RNA Expression Profile in Human Pulmonary Microvascular Endothelial Cells Exposed to Lipopolysaccharide. Cell Physiol Biochem. 2019;52(4):653-667. PubMed PMID: 30921505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Long Noncoding RNA Expression Profile in Human Pulmonary Microvascular Endothelial Cells Exposed to Lipopolysaccharide. AU - Wang,Dong, AU - Gu,Changping, AU - Liu,Mengjie, AU - Liu,Ge, AU - Liu,Huan, AU - Wang,Yuelan, PY - 2018/07/11/received PY - 2019/01/15/accepted PY - 2019/3/29/entrez PY - 2019/3/29/pubmed PY - 2019/4/12/medline KW - Acute lung injury KW - Expression profile KW - Human pulmonary microvascular endothelial cell KW - Lipopolysaccharide KW - Long noncoding RNA SP - 653 EP - 667 JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JO - Cell. Physiol. Biochem. VL - 52 IS - 4 N2 - BACKGROUND/AIMS: Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are a continuum of life-threatening lung changes. Pulmonary vascular injury is one of the most important initial causes of ALI and ARDS. However, the functions of long noncoding RNAs (lncRNAs) in pulmonary endothelial injury remain largely unknown. The aim of the present study was to determine the lncRNA expression profile of human pulmonary microvascular endothelial cells (HPMECs) exposed to lipopolysaccharide (LPS) and explore the potential functions of differentially expressed lncRNAs. METHODS: Microarray analysis was used to identify differentially expressed lncRNAs and mRNAs. Bioinformatics analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, lncRNA-mRNA coexpression network and transcription factor (TF)-lncRNA network analyses, were performed to predict the functions of significantly differentially expressed lncRNAs and mRNAs. Realtime polymerase chain reaction (PCR) was used to determine the expression of selected lncRNAs and mRNAs. RESULTS: In this study, we found that 213 lncRNAs and 212 mRNAs were significantly differentially expressed in HPMECs exposed to LPS (fold change > 2.0, p < 0.05). Furthermore, we found that mRNAs co-expressed with lncRNAs were significantly enriched in the TNF signaling pathway, the NF-κB signaling pathway, cell adhesion molecules (CAMs), cytokine-cytokine receptor interactions, and extracellular matrix (ECM)-receptor interactions. The expression levels of all but one of the selected lncRNAs and mRNAs detected by real-time PCR were similar to those detected by microarray analysis. CONCLUSION: Our data indicate that lncRNAs play an important role in LPS-induced pulmonary endothelial inflammation and barrier dysfunction and may be potential preventive and therapeutic targets for ALI and ARDS. SN - 1421-9778 UR - https://www.unboundmedicine.com/medline/citation/30921505/Analysis_of_Long_Noncoding_RNA_Expression_Profile_in_Human_Pulmonary_Microvascular_Endothelial_Cells_Exposed_to_Lipopolysaccharide_ L2 - https://www.cellphysiolbiochem.com/Articles/?DOI=10.33594/000000046 DB - PRIME DP - Unbound Medicine ER -