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Chemopreventive effect of Callistemon citrinus (Curtis) Skeels against colon cancer induced by 1,2-dimethylhydrazine in rats.
J Cancer Res Clin Oncol 2019; 145(6):1417-1426JC

Abstract

PURPOSE

Callistemon citrinus (Curtis) Skeels is a shrub native of Australia. In spite of containing an important number of bioactive compounds (1,8-cineole, limonene and α-terpineol) recognized as a potential chemotherapeutic agents, it is only used as an ornamental plant in Mexico. This study investigated the chemopreventive effect of C. citrinus leaves extract on 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis in rats.

METHODS

Twenty-four rats were divided into 3 groups of eight rats. Group 1 served as negative control, groups 2 and 3 were given subcutaneous injections of DMH (65 mg/kg b.w.) twice a week the first 2 weeks, and then one the third week. In addition, group 3 was administrated with leaves extracts (250 mg/kg b.w., orally daily) during the 22 weeks of the experiment. Animals were killed and the presence of colon tumors and aberrant crypt foci (ACF) were scored for number and distribution pattern along the colon. The activity of two-phase II enzymes quinone reductase (QR) and glutathione S-transferase (GST) was determined in the liver and three segments of the colon: proximal, middle and distal.

RESULTS

The results show that rats feed with C. citrinus leaves extract significantly reduced the size of tumors, the number of ACF and the crypt multiplicity. Additionally, C. citrinus leaves extract increased or maintained the activity of QR and GST in the different tissues as compared with DHM-treated group (p > 0.05).

CONCLUSION

This study demonstrates that Callistemon citrinus extract could have a chemopreventive effect against colon carcinogenesis.

Authors+Show Affiliations

Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo. Cd. Universitaria, 58030, Morelia, Michoacán, Mexico.Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo. Cd. Universitaria, 58030, Morelia, Michoacán, Mexico.Instituto Químico-Biológicas Universidad Michoacana de San Nicolás de Hidalgo, Edificio B-2, Cd Universitaria, Morelia, Michoacán, Mexico.Instituto Químico-Biológicas Universidad Michoacana de San Nicolás de Hidalgo, Edificio B-2, Cd Universitaria, Morelia, Michoacán, Mexico.Instituto de Investigaciones sobre los Recursos Naturales, Universidad Michoacana de San Nicolás de Hidalgo, Ave. Juanito Itzicuaro SN, 58330, Morelia, Michoacán, Mexico.Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo. Cd. Universitaria, 58030, Morelia, Michoacán, Mexico. prchavez17@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30923945

Citation

López-Mejia, Alejandro, et al. "Chemopreventive Effect of Callistemon Citrinus (Curtis) Skeels Against Colon Cancer Induced By 1,2-dimethylhydrazine in Rats." Journal of Cancer Research and Clinical Oncology, vol. 145, no. 6, 2019, pp. 1417-1426.
López-Mejia A, Ortega-Pérez LG, Godinez-Hernández D, et al. Chemopreventive effect of Callistemon citrinus (Curtis) Skeels against colon cancer induced by 1,2-dimethylhydrazine in rats. J Cancer Res Clin Oncol. 2019;145(6):1417-1426.
López-Mejia, A., Ortega-Pérez, L. G., Godinez-Hernández, D., Nateras-Marin, B., Meléndez-Herrera, E., & Rios-Chavez, P. (2019). Chemopreventive effect of Callistemon citrinus (Curtis) Skeels against colon cancer induced by 1,2-dimethylhydrazine in rats. Journal of Cancer Research and Clinical Oncology, 145(6), pp. 1417-1426. doi:10.1007/s00432-019-02905-3.
López-Mejia A, et al. Chemopreventive Effect of Callistemon Citrinus (Curtis) Skeels Against Colon Cancer Induced By 1,2-dimethylhydrazine in Rats. J Cancer Res Clin Oncol. 2019;145(6):1417-1426. PubMed PMID: 30923945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemopreventive effect of Callistemon citrinus (Curtis) Skeels against colon cancer induced by 1,2-dimethylhydrazine in rats. AU - López-Mejia,Alejandro, AU - Ortega-Pérez,Luis Gerardo, AU - Godinez-Hernández,Daniel, AU - Nateras-Marin,Blanca, AU - Meléndez-Herrera,Esperanza, AU - Rios-Chavez,Patricia, Y1 - 2019/03/28/ PY - 2018/10/24/received PY - 2019/03/22/accepted PY - 2019/3/30/pubmed PY - 2019/6/14/medline PY - 2019/3/30/entrez KW - 1,2-Dimethylhydrazine KW - Chemoprevention KW - Colon cancer KW - Glutathione S-transferase KW - Quinone reductase SP - 1417 EP - 1426 JF - Journal of cancer research and clinical oncology JO - J. Cancer Res. Clin. Oncol. VL - 145 IS - 6 N2 - PURPOSE: Callistemon citrinus (Curtis) Skeels is a shrub native of Australia. In spite of containing an important number of bioactive compounds (1,8-cineole, limonene and α-terpineol) recognized as a potential chemotherapeutic agents, it is only used as an ornamental plant in Mexico. This study investigated the chemopreventive effect of C. citrinus leaves extract on 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis in rats. METHODS: Twenty-four rats were divided into 3 groups of eight rats. Group 1 served as negative control, groups 2 and 3 were given subcutaneous injections of DMH (65 mg/kg b.w.) twice a week the first 2 weeks, and then one the third week. In addition, group 3 was administrated with leaves extracts (250 mg/kg b.w., orally daily) during the 22 weeks of the experiment. Animals were killed and the presence of colon tumors and aberrant crypt foci (ACF) were scored for number and distribution pattern along the colon. The activity of two-phase II enzymes quinone reductase (QR) and glutathione S-transferase (GST) was determined in the liver and three segments of the colon: proximal, middle and distal. RESULTS: The results show that rats feed with C. citrinus leaves extract significantly reduced the size of tumors, the number of ACF and the crypt multiplicity. Additionally, C. citrinus leaves extract increased or maintained the activity of QR and GST in the different tissues as compared with DHM-treated group (p > 0.05). CONCLUSION: This study demonstrates that Callistemon citrinus extract could have a chemopreventive effect against colon carcinogenesis. SN - 1432-1335 UR - https://www.unboundmedicine.com/medline/citation/30923945/Chemopreventive_effect_of_Callistemon_citrinus__Curtis__Skeels_against_colon_cancer_induced_by_12_dimethylhydrazine_in_rats_ L2 - https://dx.doi.org/10.1007/s00432-019-02905-3 DB - PRIME DP - Unbound Medicine ER -