Tags

Type your tag names separated by a space and hit enter

Magnolol protects Saccharomyces cerevisiae antioxidant-deficient mutants from oxidative stress and extends yeast chronological life span.
FEMS Microbiol Lett. 2019 04 01; 366(8)FM

Abstract

We investigated the protective effect of a natural polyphenol, magnolol, on Saccharomyces cerevisiae cells under oxidative stress, and during aging. Our results showed the sensitivity of S. cerevisiae antioxidant gene deficient mutants (sod1∆, sod2∆, cta1∆, ctt1∆, gtt2∆ and tsa1∆) against hydrogen peroxide (H2O2) and menadione stress was rescued by magnolol as demonstrated in spot and colony forming unit counts. Yeast cells pretreated with magnolol showed decreased intracellular oxidation, lipid peroxidation and an increased level of reduced glutathione. Further, SOD1, CTA1 and GTT2 gene expression was examined by reverse transcription-polymerase chain reaction, and was found that magnolol significantly attenuated the upregulation of SOD1 and CTA1 genes under oxidative stress. Finally, longevity of the wild type and sod1 mutant cells were extended by magnolol, and also enhance stress resistance against oxidant stress during chronological aging.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605014, India.Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605014, India.Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605014, India.Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605014, India.Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605014, India.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30924879

Citation

Subramaniyan, Subasri, et al. "Magnolol Protects Saccharomyces Cerevisiae Antioxidant-deficient Mutants From Oxidative Stress and Extends Yeast Chronological Life Span." FEMS Microbiology Letters, vol. 366, no. 8, 2019.
Subramaniyan S, Alugoju P, Sj S, et al. Magnolol protects Saccharomyces cerevisiae antioxidant-deficient mutants from oxidative stress and extends yeast chronological life span. FEMS Microbiol Lett. 2019;366(8).
Subramaniyan, S., Alugoju, P., Sj, S., Veerabhadrappa, B., & Dyavaiah, M. (2019). Magnolol protects Saccharomyces cerevisiae antioxidant-deficient mutants from oxidative stress and extends yeast chronological life span. FEMS Microbiology Letters, 366(8). https://doi.org/10.1093/femsle/fnz065
Subramaniyan S, et al. Magnolol Protects Saccharomyces Cerevisiae Antioxidant-deficient Mutants From Oxidative Stress and Extends Yeast Chronological Life Span. FEMS Microbiol Lett. 2019 04 1;366(8) PubMed PMID: 30924879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Magnolol protects Saccharomyces cerevisiae antioxidant-deficient mutants from oxidative stress and extends yeast chronological life span. AU - Subramaniyan,Subasri, AU - Alugoju,Phaniendra, AU - Sj,Sudharshan, AU - Veerabhadrappa,Bhavana, AU - Dyavaiah,Madhu, PY - 2018/11/30/received PY - 2019/03/27/accepted PY - 2019/3/30/pubmed PY - 2020/6/4/medline PY - 2019/3/30/entrez KW - aging; ROS KW - antioxidant KW - polyphenol KW - resistance JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 366 IS - 8 N2 - We investigated the protective effect of a natural polyphenol, magnolol, on Saccharomyces cerevisiae cells under oxidative stress, and during aging. Our results showed the sensitivity of S. cerevisiae antioxidant gene deficient mutants (sod1∆, sod2∆, cta1∆, ctt1∆, gtt2∆ and tsa1∆) against hydrogen peroxide (H2O2) and menadione stress was rescued by magnolol as demonstrated in spot and colony forming unit counts. Yeast cells pretreated with magnolol showed decreased intracellular oxidation, lipid peroxidation and an increased level of reduced glutathione. Further, SOD1, CTA1 and GTT2 gene expression was examined by reverse transcription-polymerase chain reaction, and was found that magnolol significantly attenuated the upregulation of SOD1 and CTA1 genes under oxidative stress. Finally, longevity of the wild type and sod1 mutant cells were extended by magnolol, and also enhance stress resistance against oxidant stress during chronological aging. SN - 1574-6968 UR - https://www.unboundmedicine.com/medline/citation/30924879/Magnolol_protects_Saccharomyces_cerevisiae_antioxidant_deficient_mutants_from_oxidative_stress_and_extends_yeast_chronological_life_span_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1093/femsle/fnz065 DB - PRIME DP - Unbound Medicine ER -