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Impact of aerobic and respirative life-style on Lactobacillus casei N87 proteome.

Abstract

Lactic acid bacteria (LAB) are used as starter, adjunct and/or probiotic cultures in fermented foods. Several species are recognized as oxygen-tolerant anaerobes, and aerobic and respiratory cultivations may provide them with physiological and technological benefits. In this light, mechanisms involved in the adaptation to aerobic and respiratory (supplementation with heme and menaquinone) growth conditions of the O2-tolerant strain Lactobacillus casei N87 were investigated by proteomics. In fact, in this bacterial strain, respiration induced an increase in biomass yield and robustness to oxidative, long-term starvation and freeze-drying stresses, while high concentrations of dissolved O2 (dO2 60%) negatively affected its growth and cell survival. Proteomic results well paralleled with physiological and metabolic features and clearly showed that aerobic life-style led to a higher abundance of several proteins involved in carbohydrate metabolism and stress response mechanisms and, concurrently, impaired the biosynthesis of proteins involved in nucleic acid formation and translation processes, thus providing evidence at molecular level of the significant damage to L.casei N87 fitness. On the contrary, the activation of respiratory pathways due to heme and menaquinone supplementation, led to a decreased amount of chaperones and other stress related proteins. These findings confirmed that respiration reduced oxidative stress condition, allowing to positively modulate the central carbohydrate and energy metabolism and improve growth and stress tolerance features. Results of this study could be potentially functional to develop competitive adjunct and probiotic cultures effectively focused on the improvement of quality of fermented foods and the promotion of human health.

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  • Authors+Show Affiliations

    ,

    Institute of Food Sciences, National Research Council (CNR-ISA), Avellino, Italy.

    ,

    School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Potenza, Italy.

    ,

    Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council (CNR-IBIOM), Bari, Italy.

    ,

    School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Potenza, Italy.

    ,

    Institute of Food Sciences, National Research Council (CNR-ISA), Avellino, Italy. Electronic address: fmazzeo@isa.cnr.it.

    Institute of Food Sciences, National Research Council (CNR-ISA), Avellino, Italy.

    Source

    MeSH

    Aerobiosis
    Heme
    Lactobacillus casei
    Oxidation-Reduction
    Oxidative Stress
    Oxygen
    Probiotics
    Proteome
    Proteomics

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30925356

    Citation

    Siciliano, Rosa Anna, et al. "Impact of Aerobic and Respirative Life-style On Lactobacillus Casei N87 Proteome." International Journal of Food Microbiology, vol. 298, 2019, pp. 51-62.
    Siciliano RA, Pannella G, Lippolis R, et al. Impact of aerobic and respirative life-style on Lactobacillus casei N87 proteome. Int J Food Microbiol. 2019;298:51-62.
    Siciliano, R. A., Pannella, G., Lippolis, R., Ricciardi, A., Mazzeo, M. F., & Zotta, T. (2019). Impact of aerobic and respirative life-style on Lactobacillus casei N87 proteome. International Journal of Food Microbiology, 298, pp. 51-62. doi:10.1016/j.ijfoodmicro.2019.03.006.
    Siciliano RA, et al. Impact of Aerobic and Respirative Life-style On Lactobacillus Casei N87 Proteome. Int J Food Microbiol. 2019 Jun 2;298:51-62. PubMed PMID: 30925356.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Impact of aerobic and respirative life-style on Lactobacillus casei N87 proteome. AU - Siciliano,Rosa Anna, AU - Pannella,Gianfranco, AU - Lippolis,Rosa, AU - Ricciardi,Annamaria, AU - Mazzeo,Maria Fiorella, AU - Zotta,Teresa, Y1 - 2019/03/12/ PY - 2018/07/12/received PY - 2019/01/10/revised PY - 2019/03/10/accepted PY - 2019/3/30/pubmed PY - 2019/5/16/medline PY - 2019/3/30/entrez KW - Aerobiosis KW - Energy/ nucleotide metabolism KW - Lactobacillus casei KW - Proteomics KW - Respiration KW - Stress proteins SP - 51 EP - 62 JF - International journal of food microbiology JO - Int. J. Food Microbiol. VL - 298 N2 - Lactic acid bacteria (LAB) are used as starter, adjunct and/or probiotic cultures in fermented foods. Several species are recognized as oxygen-tolerant anaerobes, and aerobic and respiratory cultivations may provide them with physiological and technological benefits. In this light, mechanisms involved in the adaptation to aerobic and respiratory (supplementation with heme and menaquinone) growth conditions of the O2-tolerant strain Lactobacillus casei N87 were investigated by proteomics. In fact, in this bacterial strain, respiration induced an increase in biomass yield and robustness to oxidative, long-term starvation and freeze-drying stresses, while high concentrations of dissolved O2 (dO2 60%) negatively affected its growth and cell survival. Proteomic results well paralleled with physiological and metabolic features and clearly showed that aerobic life-style led to a higher abundance of several proteins involved in carbohydrate metabolism and stress response mechanisms and, concurrently, impaired the biosynthesis of proteins involved in nucleic acid formation and translation processes, thus providing evidence at molecular level of the significant damage to L.casei N87 fitness. On the contrary, the activation of respiratory pathways due to heme and menaquinone supplementation, led to a decreased amount of chaperones and other stress related proteins. These findings confirmed that respiration reduced oxidative stress condition, allowing to positively modulate the central carbohydrate and energy metabolism and improve growth and stress tolerance features. Results of this study could be potentially functional to develop competitive adjunct and probiotic cultures effectively focused on the improvement of quality of fermented foods and the promotion of human health. SN - 1879-3460 UR - https://www.unboundmedicine.com/medline/citation/30925356/Impact_of_aerobic_and_respirative_life-style_on_Lactobacillus_casei_N87_proteome L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(18)30367-2 DB - PRIME DP - Unbound Medicine ER -