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Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA).
Harmful Algae. 2019 02; 82:9-18.HA

Abstract

Cyanobacterial blooms are intensifying global ecological hazards. The fine structure and dynamics of bloom community are critical to understanding bloom development but little understood. Here, the questions whether dominant bloomers have high diversity and whether dominant OTUs (operational taxonomical units) compete with one another were addressed. 16S rRNA gene amplicons from an annual bloom at five locations in Harsha Lake (Ohio, USA) showed cyanobacteria were the dominant phylum, and co-existing major bacterial phyla included Proteobacteria, Bacteroidetes, Actinoacteria, and Verrucomicrobia. On the genus level, the initial dominance by Dolichospermum in June yielded to Planktothrix in July, which were replaced by Microcystis and Cylindrospermopsis in August throughout the bloom. Based on the number of verified unique OTUs (a within-genus biodiversity metric), dominant genera tended to have high within-genus diversity. For example, Dolichospermum had 57 unique OTUs, Planktothrix had 36, Microcystis had 12, and Cylindrospermopsis had 4 unique OTUs. Interestingly, these different OTUs showed different dynamics and association with other OTUs. First, no between-OTU competitions were observed during the bloom cycle, and dominant OTUs were abundant throughout the bloom. Such biodiversity of OTUs and their dynamics were verified in Microcystis aeruginosa with two microcystin synthetase genes (mcyA and mcyG): the relative abundance of both genes varied during the bloom based on quantitative PCR. Two Dolichospermum circinale OTUs and one P. rubescens OTU were most abundant and persistently present throughout the entire bloom. Second, these OTUs differed in the OTUs they were associated with. Third, these OTUs tended to have different levels of association with the environmental factors, even they belonged to the same genera. These findings suggest the structure and dynamics of a cyanobacterial bloom community is complex, with only few OTUs dominating the bloom. Thus, high-resolution molecular characterization will be necessary to understand bloom development.

Authors+Show Affiliations

Institute of Hydrobiology, Jinan University, Guangzhou, Guangdong, China.Center for Fundamental and Applied Microbiomics, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA. Electronic address: hshcao@asu.edu.College of Computer Science and Technology, Jilin University, Jilin, Changchun 100012, China.College of Computer Science and Technology, Jilin University, Jilin, Changchun 100012, China.College of Pharmacy, Beihua University, Jilin, Changchun 132013, China.US EPA ORD, Cincinnati, OH 45268, USA.College of Health Solutions, Arizona State University, Scottsdale, AZ 85259, USA.Institute of Hydrobiology, Jinan University, Guangzhou, Guangdong, China.Institute of Hydrobiology, Jinan University, Guangzhou, Guangdong, China.US EPA ORD, Cincinnati, OH 45268, USA. Electronic address: Lu.jingrang@epa.gov.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30928013

Citation

Zhu, Bo, et al. "Biodiversity and Dynamics of Cyanobacterial Communities During Blooms in Temperate Lake (Harsha Lake, Ohio, USA)." Harmful Algae, vol. 82, 2019, pp. 9-18.
Zhu B, Cao H, Li G, et al. Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA). Harmful Algae. 2019;82:9-18.
Zhu, B., Cao, H., Li, G., Du, W., Xu, G., Domingo, J. S., Gu, H., Xu, N., Duan, S., & Lu, J. (2019). Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA). Harmful Algae, 82, 9-18. https://doi.org/10.1016/j.hal.2018.12.006
Zhu B, et al. Biodiversity and Dynamics of Cyanobacterial Communities During Blooms in Temperate Lake (Harsha Lake, Ohio, USA). Harmful Algae. 2019;82:9-18. PubMed PMID: 30928013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biodiversity and dynamics of cyanobacterial communities during blooms in temperate lake (Harsha Lake, Ohio, USA). AU - Zhu,Bo, AU - Cao,Huansheng, AU - Li,Gaoyang, AU - Du,Wei, AU - Xu,Guangyu, AU - Domingo,Jorge Santo, AU - Gu,Haiwei, AU - Xu,Ning, AU - Duan,Shunshan, AU - Lu,Jingrang, Y1 - 2018/12/29/ PY - 2018/08/20/received PY - 2018/12/18/revised PY - 2018/12/20/accepted PY - 2019/4/1/entrez PY - 2019/4/1/pubmed PY - 2020/2/23/medline KW - 16S rRNA gene KW - Cyanobacterial blooms KW - Dolichospermum KW - Genetic diversity KW - Harsha Lake KW - Unique operational taxonomic unit SP - 9 EP - 18 JF - Harmful algae JO - Harmful Algae VL - 82 N2 - Cyanobacterial blooms are intensifying global ecological hazards. The fine structure and dynamics of bloom community are critical to understanding bloom development but little understood. Here, the questions whether dominant bloomers have high diversity and whether dominant OTUs (operational taxonomical units) compete with one another were addressed. 16S rRNA gene amplicons from an annual bloom at five locations in Harsha Lake (Ohio, USA) showed cyanobacteria were the dominant phylum, and co-existing major bacterial phyla included Proteobacteria, Bacteroidetes, Actinoacteria, and Verrucomicrobia. On the genus level, the initial dominance by Dolichospermum in June yielded to Planktothrix in July, which were replaced by Microcystis and Cylindrospermopsis in August throughout the bloom. Based on the number of verified unique OTUs (a within-genus biodiversity metric), dominant genera tended to have high within-genus diversity. For example, Dolichospermum had 57 unique OTUs, Planktothrix had 36, Microcystis had 12, and Cylindrospermopsis had 4 unique OTUs. Interestingly, these different OTUs showed different dynamics and association with other OTUs. First, no between-OTU competitions were observed during the bloom cycle, and dominant OTUs were abundant throughout the bloom. Such biodiversity of OTUs and their dynamics were verified in Microcystis aeruginosa with two microcystin synthetase genes (mcyA and mcyG): the relative abundance of both genes varied during the bloom based on quantitative PCR. Two Dolichospermum circinale OTUs and one P. rubescens OTU were most abundant and persistently present throughout the entire bloom. Second, these OTUs differed in the OTUs they were associated with. Third, these OTUs tended to have different levels of association with the environmental factors, even they belonged to the same genera. These findings suggest the structure and dynamics of a cyanobacterial bloom community is complex, with only few OTUs dominating the bloom. Thus, high-resolution molecular characterization will be necessary to understand bloom development. SN - 1878-1470 UR - https://www.unboundmedicine.com/medline/citation/30928013/Biodiversity_and_dynamics_of_cyanobacterial_communities_during_blooms_in_temperate_lake__Harsha_Lake_Ohio_USA__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1568-9883(18)30194-X DB - PRIME DP - Unbound Medicine ER -