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Vitamin D receptor (VDR) TaqI polymorphism, vitamin D and bone mineral density in patients with inflammatory bowel diseases.
Adv Clin Exp Med 2019; 28(7):955-960AC

Abstract

BACKGROUND

A common feature in the etiology of inflammatory bowel disease (IBD) and osteoporosis is a complex genetic background. Moreover, it has been shown that some of the susceptibility loci overlap for both diseases. One of the genes that may be involved in the pathogenesis of IBD as well as decreased bone mass is the vitamin D receptor (VDR) gene.

OBJECTIVES

The aim of this study was to investigate the association of the TaqI polymorphism (rs731236, c.1056T >C) in the VDR gene with serum vitamin D concentration and bone mineral density (BMD) in patients with IBD.

MATERIAL AND METHODS

A total of 172 IBD patients (85 with Crohn's disease (CD) and 87 with ulcerative colitis (UC)) and 39 healthy controls were enrolled in the study. Polymorphism was determined with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Bone mineral density was measured at the lumbar spine (L2-L4) and the femoral neck (FN) using dual-energy x-ray absorptiometry (DEXA). Serum concentrations of 25-hydroxyvitamin D were determined using electrochemiluminescence binding assay (ECLIA).

RESULTS

Our studies revealed that serum vitamin D concentration in IBD patients was not lowered in comparison with healthy controls. Patients with CD presented more advanced osteopenia and osteoporosis. Individuals with UC carrying the TaqI tt genotype of VDR gene showed significantly higher FN BMD than carriers of TT and Tt genotypes (p = 0.02). Moreover, tt genotype was present with higher frequency in UC patients than in controls and CD patients (23% vs 7.7% and 16.5%, respectively).

CONCLUSIONS

The tt genotype may have a protective effect on BMD in UC patients.

Authors+Show Affiliations

Department of Gastroenterology, Human Nutrition and Internal Medicine, Poznan University of Medical Sciences, Poland.Department of Gastroenterology, Human Nutrition and Internal Medicine, Poznan University of Medical Sciences, Poland.Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.Department of Gastroenterology, Human Nutrition and Internal Medicine, Poznan University of Medical Sciences, Poland.Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poland.Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poland.Department of Gastroenterology, Human Nutrition and Internal Medicine, Poznan University of Medical Sciences, Poland.Department of Gastroenterology, Human Nutrition and Internal Medicine, Poznan University of Medical Sciences, Poland.Department of Computer Sciences and Statistics, Poznan University of Medical Sciences, Poland.Department of Gastroenterology, Human Nutrition and Internal Medicine, Poznan University of Medical Sciences, Poland.Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland. Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30929318

Citation

Szymczak-Tomczak, Aleksandra, et al. "Vitamin D Receptor (VDR) TaqI Polymorphism, Vitamin D and Bone Mineral Density in Patients With Inflammatory Bowel Diseases." Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University, vol. 28, no. 7, 2019, pp. 955-960.
Szymczak-Tomczak A, Krela-Kaźmierczak I, Kaczmarek-Ryś M, et al. Vitamin D receptor (VDR) TaqI polymorphism, vitamin D and bone mineral density in patients with inflammatory bowel diseases. Adv Clin Exp Med. 2019;28(7):955-960.
Szymczak-Tomczak, A., Krela-Kaźmierczak, I., Kaczmarek-Ryś, M., Hryhorowicz, S., Stawczyk-Eder, K., Szalata, M., ... Słomski, R. (2019). Vitamin D receptor (VDR) TaqI polymorphism, vitamin D and bone mineral density in patients with inflammatory bowel diseases. Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University, 28(7), pp. 955-960. doi:10.17219/acem/97376.
Szymczak-Tomczak A, et al. Vitamin D Receptor (VDR) TaqI Polymorphism, Vitamin D and Bone Mineral Density in Patients With Inflammatory Bowel Diseases. Adv Clin Exp Med. 2019;28(7):955-960. PubMed PMID: 30929318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitamin D receptor (VDR) TaqI polymorphism, vitamin D and bone mineral density in patients with inflammatory bowel diseases. AU - Szymczak-Tomczak,Aleksandra, AU - Krela-Kaźmierczak,Iwona, AU - Kaczmarek-Ryś,Marta, AU - Hryhorowicz,Szymon, AU - Stawczyk-Eder,Kamila, AU - Szalata,Marlena, AU - Skrzypczak-Zielińska,Marzena, AU - Łykowska-Szuber,Liliana, AU - Eder,Piotr, AU - Michalak,Michał, AU - Dobrowolska,Agnieszka, AU - Słomski,Ryszard, PY - 2019/4/1/pubmed PY - 2019/8/31/medline PY - 2019/4/1/entrez KW - bone mineral density KW - inflammatory bowel diseases KW - vitamin D KW - vitamin D receptor gene polymorphism SP - 955 EP - 960 JF - Advances in clinical and experimental medicine : official organ Wroclaw Medical University JO - Adv Clin Exp Med VL - 28 IS - 7 N2 - BACKGROUND: A common feature in the etiology of inflammatory bowel disease (IBD) and osteoporosis is a complex genetic background. Moreover, it has been shown that some of the susceptibility loci overlap for both diseases. One of the genes that may be involved in the pathogenesis of IBD as well as decreased bone mass is the vitamin D receptor (VDR) gene. OBJECTIVES: The aim of this study was to investigate the association of the TaqI polymorphism (rs731236, c.1056T >C) in the VDR gene with serum vitamin D concentration and bone mineral density (BMD) in patients with IBD. MATERIAL AND METHODS: A total of 172 IBD patients (85 with Crohn's disease (CD) and 87 with ulcerative colitis (UC)) and 39 healthy controls were enrolled in the study. Polymorphism was determined with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Bone mineral density was measured at the lumbar spine (L2-L4) and the femoral neck (FN) using dual-energy x-ray absorptiometry (DEXA). Serum concentrations of 25-hydroxyvitamin D were determined using electrochemiluminescence binding assay (ECLIA). RESULTS: Our studies revealed that serum vitamin D concentration in IBD patients was not lowered in comparison with healthy controls. Patients with CD presented more advanced osteopenia and osteoporosis. Individuals with UC carrying the TaqI tt genotype of VDR gene showed significantly higher FN BMD than carriers of TT and Tt genotypes (p = 0.02). Moreover, tt genotype was present with higher frequency in UC patients than in controls and CD patients (23% vs 7.7% and 16.5%, respectively). CONCLUSIONS: The tt genotype may have a protective effect on BMD in UC patients. SN - 1899-5276 UR - https://www.unboundmedicine.com/medline/citation/30929318/Vitamin_D_receptor__VDR__TaqI_polymorphism_vitamin_D_and_bone_mineral_density_in_patients_with_inflammatory_bowel_diseases_ L2 - http://www.advances.am.wroc.pl/pdf/2019/28/7/955.pdf DB - PRIME DP - Unbound Medicine ER -