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Simultaneous detection of Dengue virus, Chikungunya virus, Zika virus, Yellow fever virus and West Nile virus.
J Virol Methods. 2019 06; 268:53-55.JV

Abstract

Although certain mosquito-borne virus, such as Dengue virus (DENV), Chikungunya virus (CHIKV), Zika virus (ZIKV), Yellow fever virus (YFV) and West Nile virus (WNV), are an important public health concern in those countries where transmitter mosquitoes are endemic, several cases of travelers from those endemic countries have been recently reported in Europe. Thus, early diagnosis of these viruses is essential for patient management and adoption of preventive measures. An assay for the simultaneous detection of DENV, CHIKV, ZIKV, YFV and WNV based on a multiplex real-time (RT)-PCR and its usefulness for diagnosis in infection screenings and surveillance of arbovirus in non-endemic countries are described.

Authors+Show Affiliations

Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain. Electronic address: joseantonio.boga@sespa.es.Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30930286

Citation

Boga, José A., et al. "Simultaneous Detection of Dengue Virus, Chikungunya Virus, Zika Virus, Yellow Fever Virus and West Nile Virus." Journal of Virological Methods, vol. 268, 2019, pp. 53-55.
Boga JA, Alvarez-Arguelles ME, Rojo-Alba S, et al. Simultaneous detection of Dengue virus, Chikungunya virus, Zika virus, Yellow fever virus and West Nile virus. J Virol Methods. 2019;268:53-55.
Boga, J. A., Alvarez-Arguelles, M. E., Rojo-Alba, S., Rodríguez, M., de Oña, M., & Melón, S. (2019). Simultaneous detection of Dengue virus, Chikungunya virus, Zika virus, Yellow fever virus and West Nile virus. Journal of Virological Methods, 268, 53-55. https://doi.org/10.1016/j.jviromet.2019.03.014
Boga JA, et al. Simultaneous Detection of Dengue Virus, Chikungunya Virus, Zika Virus, Yellow Fever Virus and West Nile Virus. J Virol Methods. 2019;268:53-55. PubMed PMID: 30930286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous detection of Dengue virus, Chikungunya virus, Zika virus, Yellow fever virus and West Nile virus. AU - Boga,José A, AU - Alvarez-Arguelles,Marta E, AU - Rojo-Alba,Susana, AU - Rodríguez,Mercedes, AU - de Oña,María, AU - Melón,Santiago, Y1 - 2019/03/28/ PY - 2018/08/29/received PY - 2019/03/11/revised PY - 2019/03/27/accepted PY - 2019/4/2/pubmed PY - 2020/3/7/medline PY - 2019/4/2/entrez KW - Arbovirus KW - Diagnosis KW - Multiplex PCR SP - 53 EP - 55 JF - Journal of virological methods JO - J Virol Methods VL - 268 N2 - Although certain mosquito-borne virus, such as Dengue virus (DENV), Chikungunya virus (CHIKV), Zika virus (ZIKV), Yellow fever virus (YFV) and West Nile virus (WNV), are an important public health concern in those countries where transmitter mosquitoes are endemic, several cases of travelers from those endemic countries have been recently reported in Europe. Thus, early diagnosis of these viruses is essential for patient management and adoption of preventive measures. An assay for the simultaneous detection of DENV, CHIKV, ZIKV, YFV and WNV based on a multiplex real-time (RT)-PCR and its usefulness for diagnosis in infection screenings and surveillance of arbovirus in non-endemic countries are described. SN - 1879-0984 UR - https://www.unboundmedicine.com/medline/citation/30930286/Simultaneous_detection_of_Dengue_virus_Chikungunya_virus_Zika_virus_Yellow_fever_virus_and_West_Nile_virus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(18)30379-3 DB - PRIME DP - Unbound Medicine ER -