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CD44 Promotes Migration and Invasion of Docetaxel-Resistant Prostate Cancer Cells Likely via Induction of Hippo-Yap Signaling.
Cells. 2019 03 30; 8(4)C

Abstract

Patients receiving docetaxel developed a drug resistance within a few months. We generated docetaxel-resistant PC/DX25 and DU/DX50 CRPC cells from PC-3 and DU-145 PCa cells, respectively. We investigated the mechanism behind why PC/DX25 and DU/DX50 cells exhibited higher migration and invasion ability. Transwell assays were used to measure the migration and invasion of PCa cell. Fluorescence activated cell sorter (FACS) analysis was used to determine the population of cancer stem cell (CSC)-like cell. Micro-Western Array (MWA) was used to study the changes of the protein profile. FACS analysis revealed that PC/DX25 cells and DU/DX50 cells contain higher CD44+ population. MWA and Western blotting assay revealed that protein expression of CD44, YAP, CYR61, CTGF, phospho-ERK1/2 T202/Y204, ERK and vimentin was elevated in PC/DX25 cells. Knockdown of CD44 or YAP suppressed migration and invasion of PC/DX25 and DU/DX50 cells. Knockdown of CD44 decreased expression of YAP, CTGF and CYR61 but increased phosphorylation of S127 on YAP. CD44 knockdown also suppressed protein level of AKT, phospho-AKT T308, phospho-ERK1/2 T202/Y204 and vimentin. CD44 promotes migration and invasion of docetaxel-resistant PCa cells probably via induction of Hippo-Yap signaling pathway and CD44/YAP pathway may be a therapeutic target for docetaxel-resistant PCa.

Authors+Show Affiliations

Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli 35053, Taiwan. krad0127@gmail.com. Institute of Biotechnology, National Tsing Hua University, Hsinchu City 30013, Taiwan. krad0127@gmail.com.Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli 35053, Taiwan. cylin071@nhri.org.tw.Nation Institute of Cancer Research, National Health Research Institutes, Miaoli 35053, Taiwan. wyliao777@gmail.com.Department of Biochemistry, Kaohsiung Medical University, Kaohsiung City 80708, Taiwan. cliff@gap.kmu.edu.tw. Department of Medical Research, Kaohsiung Medical University, Kaohsiung City 80708, Taiwan. cliff@gap.kmu.edu.tw.Institute of Biotechnology, National Tsing Hua University, Hsinchu City 30013, Taiwan. hdwang@life.nthu.edu.tw. Department of Life Science, National Tsing Hua University, Hsinchu City 30013, Taiwan. hdwang@life.nthu.edu.tw.Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli 35053, Taiwan. cpchuu@nhri.org.tw. Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan. cpchuu@nhri.org.tw. Ph.D. Program for Aging, China Medical University, Taichung 40402, Taiwan. cpchuu@nhri.org.tw. Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan. cpchuu@nhri.org.tw.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30935014

Citation

Lai, Chih-Jen, et al. "CD44 Promotes Migration and Invasion of Docetaxel-Resistant Prostate Cancer Cells Likely Via Induction of Hippo-Yap Signaling." Cells, vol. 8, no. 4, 2019.
Lai CJ, Lin CY, Liao WY, et al. CD44 Promotes Migration and Invasion of Docetaxel-Resistant Prostate Cancer Cells Likely via Induction of Hippo-Yap Signaling. Cells. 2019;8(4).
Lai, C. J., Lin, C. Y., Liao, W. Y., Hour, T. C., Wang, H. D., & Chuu, C. P. (2019). CD44 Promotes Migration and Invasion of Docetaxel-Resistant Prostate Cancer Cells Likely via Induction of Hippo-Yap Signaling. Cells, 8(4). https://doi.org/10.3390/cells8040295
Lai CJ, et al. CD44 Promotes Migration and Invasion of Docetaxel-Resistant Prostate Cancer Cells Likely Via Induction of Hippo-Yap Signaling. Cells. 2019 03 30;8(4) PubMed PMID: 30935014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD44 Promotes Migration and Invasion of Docetaxel-Resistant Prostate Cancer Cells Likely via Induction of Hippo-Yap Signaling. AU - Lai,Chih-Jen, AU - Lin,Ching-Yu, AU - Liao,Wen-Ying, AU - Hour,Tzyh-Chyuan, AU - Wang,Horng-Dar, AU - Chuu,Chih-Pin, Y1 - 2019/03/30/ PY - 2019/02/26/received PY - 2019/03/19/revised PY - 2019/03/27/accepted PY - 2019/4/3/entrez PY - 2019/4/3/pubmed PY - 2019/4/3/medline KW - CD44 KW - DU-145 KW - PC-3 KW - YAP KW - docetaxel-resistant KW - invasion KW - migration KW - prostate cancer JF - Cells JO - Cells VL - 8 IS - 4 N2 - Patients receiving docetaxel developed a drug resistance within a few months. We generated docetaxel-resistant PC/DX25 and DU/DX50 CRPC cells from PC-3 and DU-145 PCa cells, respectively. We investigated the mechanism behind why PC/DX25 and DU/DX50 cells exhibited higher migration and invasion ability. Transwell assays were used to measure the migration and invasion of PCa cell. Fluorescence activated cell sorter (FACS) analysis was used to determine the population of cancer stem cell (CSC)-like cell. Micro-Western Array (MWA) was used to study the changes of the protein profile. FACS analysis revealed that PC/DX25 cells and DU/DX50 cells contain higher CD44+ population. MWA and Western blotting assay revealed that protein expression of CD44, YAP, CYR61, CTGF, phospho-ERK1/2 T202/Y204, ERK and vimentin was elevated in PC/DX25 cells. Knockdown of CD44 or YAP suppressed migration and invasion of PC/DX25 and DU/DX50 cells. Knockdown of CD44 decreased expression of YAP, CTGF and CYR61 but increased phosphorylation of S127 on YAP. CD44 knockdown also suppressed protein level of AKT, phospho-AKT T308, phospho-ERK1/2 T202/Y204 and vimentin. CD44 promotes migration and invasion of docetaxel-resistant PCa cells probably via induction of Hippo-Yap signaling pathway and CD44/YAP pathway may be a therapeutic target for docetaxel-resistant PCa. SN - 2073-4409 UR - https://www.unboundmedicine.com/medline/citation/30935014/CD44_Promotes_Migration_and_Invasion_of_Docetaxel_Resistant_Prostate_Cancer_Cells_Likely_via_Induction_of_Hippo_Yap_Signaling_ L2 - http://www.mdpi.com/resolver?pii=cells8040295 DB - PRIME DP - Unbound Medicine ER -