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Matrine improves diabetic cardiomyopathy through TGF-β-induced protein kinase RNA-like endoplasmic reticulum kinase signaling pathway.
J Cell Biochem. 2019 08; 120(8):13573-13582.JC

Abstract

BACKGROUND

Matrine might play a vital role in cardiovascular diseases progression and treatment.

OBJECTIVES

We aimed to explore the protective effects and potential mechanism of matrine against diabetic cardiomyopathy (DCM) in rat model.

METHOD

A rat model of DCM was induced by streptozotocin, which were then divided into two groups and treated with matrine. Inflammatory cytokines were investigated in serum and myocardial cells after matrine administration. The effects of matrine on cardiac reactive oxygen species (ROS) generation, Malondialdehyde (MDA) levels, and Glutathione peroxidase (GPx), PPARγ1 activity were detected in myocardial cells. The protein kinase RNA-like endoplasmic reticulum kinase (PERK) signal pathway in endoplasmic reticulum stress was studied to elaborated protective effects of matrine in DCM rat by Western blot analysis. Fasting blood glucose and hemodynamic parameters were analyzed after treatment with matrine.

RESULTS

Matrine-inhibited expression levels of inflammatory cytokines of tumor necrosis factor alpha (TNF-α) and interleukin 6. Matrine administration decreased ROS generation, MDA, and transforming growth factor beta levels, and Peroxisome proliferator-activated receptor beta (PPARβ) and Peroxisome proliferator-activated receptorγ 1 (PPARγ1) activity. Matrine administration also significantly inhibited PERK expression. Endogenic expression of PERK canceled matrine-induced apoptosis of myocardial cells. Notably, treatment with matrine significantly decreased nonfasting blood glucose levels and improved hemodynamic parameters of DCM rat.

CONCLUSIONS

Matrine may be a promising agent for the treatment of DCM.

Authors+Show Affiliations

Department of Cardiology, Dezhou People's Hospital, Dezhou, China.Department of Nephrology, Dezhou People's Hospital, Dezhou, China.Department of Cardiology, Dezhou People's Hospital, Dezhou, China.Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30938856

Citation

Hou, Huijuan, et al. "Matrine Improves Diabetic Cardiomyopathy Through TGF-β-induced Protein Kinase RNA-like Endoplasmic Reticulum Kinase Signaling Pathway." Journal of Cellular Biochemistry, vol. 120, no. 8, 2019, pp. 13573-13582.
Hou H, Zhang Q, Dong H, et al. Matrine improves diabetic cardiomyopathy through TGF-β-induced protein kinase RNA-like endoplasmic reticulum kinase signaling pathway. J Cell Biochem. 2019;120(8):13573-13582.
Hou, H., Zhang, Q., Dong, H., & Ge, Z. (2019). Matrine improves diabetic cardiomyopathy through TGF-β-induced protein kinase RNA-like endoplasmic reticulum kinase signaling pathway. Journal of Cellular Biochemistry, 120(8), 13573-13582. https://doi.org/10.1002/jcb.28632
Hou H, et al. Matrine Improves Diabetic Cardiomyopathy Through TGF-β-induced Protein Kinase RNA-like Endoplasmic Reticulum Kinase Signaling Pathway. J Cell Biochem. 2019;120(8):13573-13582. PubMed PMID: 30938856.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrine improves diabetic cardiomyopathy through TGF-β-induced protein kinase RNA-like endoplasmic reticulum kinase signaling pathway. AU - Hou,Huijuan, AU - Zhang,Qianqian, AU - Dong,Hongwei, AU - Ge,Zhiming, Y1 - 2019/04/02/ PY - 2018/11/18/received PY - 2019/01/28/revised PY - 2019/01/30/accepted PY - 2019/4/3/pubmed PY - 2020/8/15/medline PY - 2019/4/3/entrez KW - apoptosis KW - diabetic cardiomyopathy (DCM) KW - endoplasmic reticulum (ER) stress KW - matrine KW - protein kinase RNA-like endoplasmic reticulum kinase expression (PERK) pathway KW - transforming growth factor beta (TGF-β) SP - 13573 EP - 13582 JF - Journal of cellular biochemistry JO - J Cell Biochem VL - 120 IS - 8 N2 - BACKGROUND: Matrine might play a vital role in cardiovascular diseases progression and treatment. OBJECTIVES: We aimed to explore the protective effects and potential mechanism of matrine against diabetic cardiomyopathy (DCM) in rat model. METHOD: A rat model of DCM was induced by streptozotocin, which were then divided into two groups and treated with matrine. Inflammatory cytokines were investigated in serum and myocardial cells after matrine administration. The effects of matrine on cardiac reactive oxygen species (ROS) generation, Malondialdehyde (MDA) levels, and Glutathione peroxidase (GPx), PPARγ1 activity were detected in myocardial cells. The protein kinase RNA-like endoplasmic reticulum kinase (PERK) signal pathway in endoplasmic reticulum stress was studied to elaborated protective effects of matrine in DCM rat by Western blot analysis. Fasting blood glucose and hemodynamic parameters were analyzed after treatment with matrine. RESULTS: Matrine-inhibited expression levels of inflammatory cytokines of tumor necrosis factor alpha (TNF-α) and interleukin 6. Matrine administration decreased ROS generation, MDA, and transforming growth factor beta levels, and Peroxisome proliferator-activated receptor beta (PPARβ) and Peroxisome proliferator-activated receptorγ 1 (PPARγ1) activity. Matrine administration also significantly inhibited PERK expression. Endogenic expression of PERK canceled matrine-induced apoptosis of myocardial cells. Notably, treatment with matrine significantly decreased nonfasting blood glucose levels and improved hemodynamic parameters of DCM rat. CONCLUSIONS: Matrine may be a promising agent for the treatment of DCM. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/30938856/Matrine_improves_diabetic_cardiomyopathy_through_TGF_β_induced_protein_kinase_RNA_like_endoplasmic_reticulum_kinase_signaling_pathway_ L2 - https://doi.org/10.1002/jcb.28632 DB - PRIME DP - Unbound Medicine ER -