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Paraoxonase 1 Was Negatively Associated With Intervertebral Disc Degeneration.
Spine (Phila Pa 1976) 2019; 44(18):E1053-E1062S

Abstract

STUDY DESIGN

An in vivo and in vitro study of the correlation between Paraoxonase 1 (PON1) and intervertebral disc degeneration (IVDD).

OBJECTIVE

The aim of this study is to clarify the expression and role of PON1 on the process of IVDD.

SUMMARY OF BACKGROUND DATA

IVDD is responsible for most of the spinal degenerative diseases. Inflammation and oxidative stress can deteriorate the living environment of nucleus pulposus (NP) cells, leading to IVDD. PON1 is an enzyme reported to have anti-inflammatory and anti-oxidative effects. There is no study about the correlation of PON1 expression with IVDD.

METHODS

Immunohistochemical (IHC), hematoxylin and eosin (H&E) staining, and Western blot examined the expression of PON1 in 88 human disc samples (male: female 43: 45) and rat models (n = 5 each group). The level of PON1 is measured in the tumor necrosis factor (TNF)-α and oxidative stress (H2O2)-induced degenerative NP cell models using Western blot and reverse transcription-polymerase chain reaction (RT-qPCR). The TNF-α, interleukin (IL)-1β, Mito superoxide (SOX), aggrecan, and collagen II are detected in nucleus pulposus (NP) cells transfected with si-RNA of PON1 using Enzyme-Linked Immunosorbent Assay (ELISA), mitoSOX staining Western blot, and RT-qPCR.

RESULTS

The expression of PON1 is significantly suppressed in human and rat degenerative intervertebral discs. The level of PON1 is significantly decreased in TNF-α and oxidative stress (H2O2)-induced degenerative NP cell models. ELISA results show that the level of TNF-α and IL-1β obviously increased; Mito SOX staining indicates that the Mito SOX fluorescence significantly increased, and the expression of aggrecan and collagen reduced in NP cells transfected with si-RNA of PON1.

CONCLUSION

Our study indicates that low PON1 expression is predictive of severe IVDD; PON1 plays an important role of keeping the homeostatic balance of intervertebral discs, and therapeutic approach regarding PON1 may be helpful to alleviate IVDD in the future.

LEVEL OF EVIDENCE

N/A.

Authors+Show Affiliations

Department of Spine Surgery, the First Affiliated Hospital and Orthopedic Research Institute of Sun Yat-sen University, Guangzhou, China.Department of Spine Surgery, the First Affiliated Hospital and Orthopedic Research Institute of Sun Yat-sen University, Guangzhou, China.Department of Spine Surgery, the First Affiliated Hospital and Orthopedic Research Institute of Sun Yat-sen University, Guangzhou, China.Department of Pediatric Intensive Care Unit, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.Department of Spine Surgery, the First Affiliated Hospital and Orthopedic Research Institute of Sun Yat-sen University, Guangzhou, China.Department of Spine Surgery, the First Affiliated Hospital and Orthopedic Research Institute of Sun Yat-sen University, Guangzhou, China.Department of Spine Surgery, the First Affiliated Hospital and Orthopedic Research Institute of Sun Yat-sen University, Guangzhou, China.Department of Spine Surgery, the First Affiliated Hospital and Orthopedic Research Institute of Sun Yat-sen University, Guangzhou, China. Department of Pain Research Center, Zhongshan Medical School, Sun Yat-Sen University, Guangdong, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30946296

Citation

Chen, Fan, et al. "Paraoxonase 1 Was Negatively Associated With Intervertebral Disc Degeneration." Spine, vol. 44, no. 18, 2019, pp. E1053-E1062.
Chen F, Liu H, Li Z, et al. Paraoxonase 1 Was Negatively Associated With Intervertebral Disc Degeneration. Spine. 2019;44(18):E1053-E1062.
Chen, F., Liu, H., Li, Z., Pei, Y., Wang, H., Zhang, J., ... Zheng, Z. (2019). Paraoxonase 1 Was Negatively Associated With Intervertebral Disc Degeneration. Spine, 44(18), pp. E1053-E1062. doi:10.1097/BRS.0000000000003059.
Chen F, et al. Paraoxonase 1 Was Negatively Associated With Intervertebral Disc Degeneration. Spine. 2019;44(18):E1053-E1062. PubMed PMID: 30946296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Paraoxonase 1 Was Negatively Associated With Intervertebral Disc Degeneration. AU - Chen,Fan, AU - Liu,Hui, AU - Li,Zemin, AU - Pei,Yuxin, AU - Wang,Hua, AU - Zhang,Jian, AU - Wang,Jianru, AU - Zheng,Zhaomin, PY - 2019/4/5/pubmed PY - 2019/4/5/medline PY - 2019/4/5/entrez SP - E1053 EP - E1062 JF - Spine JO - Spine VL - 44 IS - 18 N2 - STUDY DESIGN: An in vivo and in vitro study of the correlation between Paraoxonase 1 (PON1) and intervertebral disc degeneration (IVDD). OBJECTIVE: The aim of this study is to clarify the expression and role of PON1 on the process of IVDD. SUMMARY OF BACKGROUND DATA: IVDD is responsible for most of the spinal degenerative diseases. Inflammation and oxidative stress can deteriorate the living environment of nucleus pulposus (NP) cells, leading to IVDD. PON1 is an enzyme reported to have anti-inflammatory and anti-oxidative effects. There is no study about the correlation of PON1 expression with IVDD. METHODS: Immunohistochemical (IHC), hematoxylin and eosin (H&E) staining, and Western blot examined the expression of PON1 in 88 human disc samples (male: female 43: 45) and rat models (n = 5 each group). The level of PON1 is measured in the tumor necrosis factor (TNF)-α and oxidative stress (H2O2)-induced degenerative NP cell models using Western blot and reverse transcription-polymerase chain reaction (RT-qPCR). The TNF-α, interleukin (IL)-1β, Mito superoxide (SOX), aggrecan, and collagen II are detected in nucleus pulposus (NP) cells transfected with si-RNA of PON1 using Enzyme-Linked Immunosorbent Assay (ELISA), mitoSOX staining Western blot, and RT-qPCR. RESULTS: The expression of PON1 is significantly suppressed in human and rat degenerative intervertebral discs. The level of PON1 is significantly decreased in TNF-α and oxidative stress (H2O2)-induced degenerative NP cell models. ELISA results show that the level of TNF-α and IL-1β obviously increased; Mito SOX staining indicates that the Mito SOX fluorescence significantly increased, and the expression of aggrecan and collagen reduced in NP cells transfected with si-RNA of PON1. CONCLUSION: Our study indicates that low PON1 expression is predictive of severe IVDD; PON1 plays an important role of keeping the homeostatic balance of intervertebral discs, and therapeutic approach regarding PON1 may be helpful to alleviate IVDD in the future. LEVEL OF EVIDENCE: N/A. SN - 1528-1159 UR - https://www.unboundmedicine.com/medline/citation/30946296/Paraoxonase_1_Was_Negatively_Associated_With_Intervertebral_Disc_Degeneration_ L2 - http://dx.doi.org/10.1097/BRS.0000000000003059 DB - PRIME DP - Unbound Medicine ER -