Tags

Type your tag names separated by a space and hit enter

Molecular characteristic analysis for the VP1 region of coxsackievirus A6 strains isolated in Jiujiang area, China, from 2012 to 2013.
Medicine (Baltimore). 2019 Apr; 98(14):e15077.M

Abstract

BACKGROUND

Enterovirus 71 (EV-A71) and Coxsackievirus A16 (CV-A16) are the most common causative agents causing hand, foot, and mouth disease (HFMD). However, coxsackievirus A6 (CV-A6), previously largely ignored, became the predominant pathogen in China in 2012. The objective of this study is to investigate the genetic characteristics and molecular epidemiology of HFMD caused by CV-A6 to guide the diagnosis and treatment of the disease, as well as disease prevention.

MATERIAL AND METHODS

A total of 138 suspected HFMD cases were enrolled in this study and analyses based on complete VP1 nucleotide sequences were performed to determine the evolutionary trajectory of emerging CV-A6.

RESULTS

Among 138 samples in Jiujiang, 125 (90.58%) were positive for enterovirus, the most frequently presented serotypes were CV-A6 (77, 61.60%), CV-A16 (28, 22.40%), EV-A71 (6, 4.80%) and untyped enteroviruses (14, 11.20%). Seventy-seven CV-A6 positive specimens were analyzed for the complete VP1 sequences by sequencing and 36 representative isolates were selected to perform nucleotide sequence similarity analysis. The results showed that 36 strains isolated from HFMD patients were clustered closely to the mainland China and were far from prototype strain CV-A6/Gdula (AY421764) and other international subtypes. Moreover, phylogenetic analysis of the VP1 gene revealed that 36 circulating strains were not significantly concentrated in one branch, but were widely distributed in each branch.

CONCLUSIONS

Continuous surveillance of HFMD etiological agents other than EV-A71 and CV-A16 is necessary. CV-A6 is emerging as the most common pathogen causing HFMD. Closely monitoring the magnitude and trend of CV-A6 epidemic and the trend of pathogenic spectrum changes can provide scientific basis for this disease prevention and control to the department of disease control.

Authors+Show Affiliations

Department of Immunology, Bengbu Medical College, Bengbu, Anhui. Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui.The School of Basic Medical Science, Jiujiang University, Jiujiang Key Laboratory of Translational Medicine, Jiujiang, Jiangxi.Department of Clinical Laboratory, Bengbu Medical College, Bengbu, Anhui. Department of Laboratory Medicine, Bengbu Medical College, Bengbu, Anhui.Department of Clinical Laboratory, Bengbu Medical College, Bengbu, Anhui. Department of Laboratory Medicine, Bengbu Medical College, Bengbu, Anhui.Anhui Clinical and Preclinical Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui.Department of Histology and Embryology, Bengbu Medical College, Bengbu, Anhui, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30946358

Citation

Wang, Hongtao, et al. "Molecular Characteristic Analysis for the VP1 Region of Coxsackievirus A6 Strains Isolated in Jiujiang Area, China, From 2012 to 2013." Medicine, vol. 98, no. 14, 2019, pp. e15077.
Wang H, Yu W, Xu T, et al. Molecular characteristic analysis for the VP1 region of coxsackievirus A6 strains isolated in Jiujiang area, China, from 2012 to 2013. Medicine (Baltimore). 2019;98(14):e15077.
Wang, H., Yu, W., Xu, T., Li, Y., Wang, X., & Sun, M. (2019). Molecular characteristic analysis for the VP1 region of coxsackievirus A6 strains isolated in Jiujiang area, China, from 2012 to 2013. Medicine, 98(14), e15077. https://doi.org/10.1097/MD.0000000000015077
Wang H, et al. Molecular Characteristic Analysis for the VP1 Region of Coxsackievirus A6 Strains Isolated in Jiujiang Area, China, From 2012 to 2013. Medicine (Baltimore). 2019;98(14):e15077. PubMed PMID: 30946358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characteristic analysis for the VP1 region of coxsackievirus A6 strains isolated in Jiujiang area, China, from 2012 to 2013. AU - Wang,Hongtao, AU - Yu,Wenmin, AU - Xu,Tao, AU - Li,Yuyun, AU - Wang,Xiaojing, AU - Sun,Meiqun, PY - 2019/4/5/entrez PY - 2019/4/5/pubmed PY - 2019/4/13/medline SP - e15077 EP - e15077 JF - Medicine JO - Medicine (Baltimore) VL - 98 IS - 14 N2 - BACKGROUND: Enterovirus 71 (EV-A71) and Coxsackievirus A16 (CV-A16) are the most common causative agents causing hand, foot, and mouth disease (HFMD). However, coxsackievirus A6 (CV-A6), previously largely ignored, became the predominant pathogen in China in 2012. The objective of this study is to investigate the genetic characteristics and molecular epidemiology of HFMD caused by CV-A6 to guide the diagnosis and treatment of the disease, as well as disease prevention. MATERIAL AND METHODS: A total of 138 suspected HFMD cases were enrolled in this study and analyses based on complete VP1 nucleotide sequences were performed to determine the evolutionary trajectory of emerging CV-A6. RESULTS: Among 138 samples in Jiujiang, 125 (90.58%) were positive for enterovirus, the most frequently presented serotypes were CV-A6 (77, 61.60%), CV-A16 (28, 22.40%), EV-A71 (6, 4.80%) and untyped enteroviruses (14, 11.20%). Seventy-seven CV-A6 positive specimens were analyzed for the complete VP1 sequences by sequencing and 36 representative isolates were selected to perform nucleotide sequence similarity analysis. The results showed that 36 strains isolated from HFMD patients were clustered closely to the mainland China and were far from prototype strain CV-A6/Gdula (AY421764) and other international subtypes. Moreover, phylogenetic analysis of the VP1 gene revealed that 36 circulating strains were not significantly concentrated in one branch, but were widely distributed in each branch. CONCLUSIONS: Continuous surveillance of HFMD etiological agents other than EV-A71 and CV-A16 is necessary. CV-A6 is emerging as the most common pathogen causing HFMD. Closely monitoring the magnitude and trend of CV-A6 epidemic and the trend of pathogenic spectrum changes can provide scientific basis for this disease prevention and control to the department of disease control. SN - 1536-5964 UR - https://www.unboundmedicine.com/medline/citation/30946358/Molecular_characteristic_analysis_for_the_VP1_region_of_coxsackievirus_A6_strains_isolated_in_Jiujiang_area_China_from_2012_to_2013_ L2 - https://doi.org/10.1097/MD.0000000000015077 DB - PRIME DP - Unbound Medicine ER -