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Antibiotic resistance pattern and virulence genesin avian pathogenic Escherichia coli (APEC) from different breeding systems.
Vet Ital 2019; 55(1):26-33VI

Abstract

Colibacillosis is the most frequent bacterial disease in avian species and antimicrobials are the main weapon to reduce incidence and mortality associated to it. However, indiscriminate use of antibiotics may lead to therapy failure and economic losses for the breeder. The aims of this study were to, determine the antibiotic resistance of Escherichia coli isolates, evaluate the correlation between E. coli isolation and systems of breeding included in this study, and identify the avian pathogenic E.coli (APEC) amongst the E. coli strains isolated. A total of 51 E. coli strains were subjected to antimicrobial susceptibility test and they were screened for the presence of virulence genes through PCR. Resistance was most frequently detected against ampicillin and nalidixic acid meanwhile E. coli isolates showed less resistance to the cephalosporins. Overall, 40% of the isolates showed resistance to at least three or more antimicrobials and 16/51 isolates were defined APEC strains. The virulence genes iucD, cvi/cva, irp2 and iss were detected from all 16 APEC strains. The virulence genes tsh, vat, papC, and astA were detected from 11, 7, 5 and 3 APEC strains, respectively. Results demonstrated the importance of studies on APEC and antibiotic resistance genes in Italy, and it was shown that the systems of breeding might influence the antibiotic resistanc.

Authors+Show Affiliations

Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche 'Togo Rosati', Via G. Salvemini 1, 06126 Perugia, Italy. elisa.sgariglia86@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30951179

Citation

Sgariglia, Elisa, et al. "Antibiotic Resistance Pattern and Virulence Genesin Avian Pathogenic Escherichia Coli (APEC) From Different Breeding Systems." Veterinaria Italiana, vol. 55, no. 1, 2019, pp. 26-33.
Sgariglia E, Aconiti Mandolini N, Napoleoni M, et al. Antibiotic resistance pattern and virulence genesin avian pathogenic Escherichia coli (APEC) from different breeding systems. Vet Ital. 2019;55(1):26-33.
Sgariglia, E., Aconiti Mandolini, N., Napoleoni, M., Medici, L., Fraticelli, R., Conquista, M., ... Perugini, G. (2019). Antibiotic resistance pattern and virulence genesin avian pathogenic Escherichia coli (APEC) from different breeding systems. Veterinaria Italiana, 55(1), pp. 26-33. doi:10.12834/VetIt.1617.8701.1.
Sgariglia E, et al. Antibiotic Resistance Pattern and Virulence Genesin Avian Pathogenic Escherichia Coli (APEC) From Different Breeding Systems. Vet Ital. 2019 Mar 31;55(1):26-33. PubMed PMID: 30951179.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibiotic resistance pattern and virulence genesin avian pathogenic Escherichia coli (APEC) from different breeding systems. AU - Sgariglia,Elisa, AU - Aconiti Mandolini,Nicholas, AU - Napoleoni,Maira, AU - Medici,Laura, AU - Fraticelli,Roberta, AU - Conquista,Michela, AU - Gianfelici,Paola, AU - Staffolani,Monica, AU - Fisichella,Stefano, AU - Capuccella,Marinella, AU - Sargenti,Marta, AU - Perugini,Gianni, Y1 - 2019/03/31/ PY - 2018/06/13/received PY - 2018/09/26/accepted PY - 2019/4/6/entrez SP - 26 EP - 33 JF - Veterinaria italiana JO - Vet. Ital. VL - 55 IS - 1 N2 - Colibacillosis is the most frequent bacterial disease in avian species and antimicrobials are the main weapon to reduce incidence and mortality associated to it. However, indiscriminate use of antibiotics may lead to therapy failure and economic losses for the breeder. The aims of this study were to, determine the antibiotic resistance of Escherichia coli isolates, evaluate the correlation between E. coli isolation and systems of breeding included in this study, and identify the avian pathogenic E.coli (APEC) amongst the E. coli strains isolated. A total of 51 E. coli strains were subjected to antimicrobial susceptibility test and they were screened for the presence of virulence genes through PCR. Resistance was most frequently detected against ampicillin and nalidixic acid meanwhile E. coli isolates showed less resistance to the cephalosporins. Overall, 40% of the isolates showed resistance to at least three or more antimicrobials and 16/51 isolates were defined APEC strains. The virulence genes iucD, cvi/cva, irp2 and iss were detected from all 16 APEC strains. The virulence genes tsh, vat, papC, and astA were detected from 11, 7, 5 and 3 APEC strains, respectively. Results demonstrated the importance of studies on APEC and antibiotic resistance genes in Italy, and it was shown that the systems of breeding might influence the antibiotic resistanc. SN - 1828-1427 UR - https://www.unboundmedicine.com/medline/citation/30951179/Antibiotic_resistance_pattern_and_virulence_genesin_avian_pathogenic_Escherichia_coli_(APEC)_from_different_breeding_systems L2 - http://www.izs.it/vet_italiana/2019/55_1/26.htm DB - PRIME DP - Unbound Medicine ER -