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Comparative analysis of Naja kaouthia venom from North-East India and Bangladesh and its cross reactivity with Indian polyvalent antivenoms.
Toxicon. 2019 Jun; 164:31-43.T

Abstract

Naja kaouthia is one of the most prevalent medically important snakes of North East India and Bangladesh responsible for most of the bite cases. In this study, an attempt was made to decipher venom variation of Naja kaouthia venom from North East India and Bangladesh. Using multidimensional methods including reverse phase HPLC, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-PAGE) and two-dimensional gel electrophoresis (2D-PAGE), the quantitative differences in venom composition have been revealed. Moreover, tested in-vitro biochemical and biological activities also exhibited differences which could be due to venom variability. Furthermore, neutralization efficacy of commercially available Indian polyvalent antivenoms (Vins, Bharat Serum, Haffkine) was evaluated and the results displayed significant differences in neutralizing efficacy between the antivenoms. Immunoblotting experiments showed antivenom molecules cross reacted with high molecular mass components while poorly reacted towards low molecular mass proteins. Immuno-depletion study demonstrated that Vins polyvalent antivenom was poor in immunocapturing the venom proteins of both North East Indian and Bangladesh origin Naja kaouthia at the ratio of 1:16 (venom: antivenom).

Authors+Show Affiliations

Molecular Toxinology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Assam, 784028, India.Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, 6205, Bangladesh.Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, 6205, Bangladesh.Cell and Molecular Biology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Assam, 784028, India.Department of Biotechnology, National Institute of Technology Durgapur, West Bengal, 713209, India.Molecular Toxinology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Assam, 784028, India. Electronic address: doley@tezu.ernet.in.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

30953661

Citation

Deka, Archana, et al. "Comparative Analysis of Naja Kaouthia Venom From North-East India and Bangladesh and Its Cross Reactivity With Indian Polyvalent Antivenoms." Toxicon : Official Journal of the International Society On Toxinology, vol. 164, 2019, pp. 31-43.
Deka A, Reza MA, Faisal Hoque KM, et al. Comparative analysis of Naja kaouthia venom from North-East India and Bangladesh and its cross reactivity with Indian polyvalent antivenoms. Toxicon. 2019;164:31-43.
Deka, A., Reza, M. A., Faisal Hoque, K. M., Deka, K., Saha, S., & Doley, R. (2019). Comparative analysis of Naja kaouthia venom from North-East India and Bangladesh and its cross reactivity with Indian polyvalent antivenoms. Toxicon : Official Journal of the International Society On Toxinology, 164, 31-43. https://doi.org/10.1016/j.toxicon.2019.03.025
Deka A, et al. Comparative Analysis of Naja Kaouthia Venom From North-East India and Bangladesh and Its Cross Reactivity With Indian Polyvalent Antivenoms. Toxicon. 2019;164:31-43. PubMed PMID: 30953661.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative analysis of Naja kaouthia venom from North-East India and Bangladesh and its cross reactivity with Indian polyvalent antivenoms. AU - Deka,Archana, AU - Reza,Md Abu, AU - Faisal Hoque,Kazi Md, AU - Deka,Kamalakshi, AU - Saha,Sougata, AU - Doley,Robin, Y1 - 2019/04/03/ PY - 2018/12/21/received PY - 2019/03/26/revised PY - 2019/03/31/accepted PY - 2019/4/7/pubmed PY - 2019/9/24/medline PY - 2019/4/7/entrez KW - Cross-reactivity KW - Immuno-depletion study KW - Naja kaouthia KW - Neutralization KW - Polyvalent antivenoms SP - 31 EP - 43 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 164 N2 - Naja kaouthia is one of the most prevalent medically important snakes of North East India and Bangladesh responsible for most of the bite cases. In this study, an attempt was made to decipher venom variation of Naja kaouthia venom from North East India and Bangladesh. Using multidimensional methods including reverse phase HPLC, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-PAGE) and two-dimensional gel electrophoresis (2D-PAGE), the quantitative differences in venom composition have been revealed. Moreover, tested in-vitro biochemical and biological activities also exhibited differences which could be due to venom variability. Furthermore, neutralization efficacy of commercially available Indian polyvalent antivenoms (Vins, Bharat Serum, Haffkine) was evaluated and the results displayed significant differences in neutralizing efficacy between the antivenoms. Immunoblotting experiments showed antivenom molecules cross reacted with high molecular mass components while poorly reacted towards low molecular mass proteins. Immuno-depletion study demonstrated that Vins polyvalent antivenom was poor in immunocapturing the venom proteins of both North East Indian and Bangladesh origin Naja kaouthia at the ratio of 1:16 (venom: antivenom). SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/30953661/Comparative_analysis_of_Naja_kaouthia_venom_from_North_East_India_and_Bangladesh_and_its_cross_reactivity_with_Indian_polyvalent_antivenoms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(19)30110-2 DB - PRIME DP - Unbound Medicine ER -