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Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.
Proc Natl Acad Sci U S A. 1986 Nov; 83(21):8122-6.PN

Abstract

DNA coding for bacteriophage T7 RNA polymerase was ligated to a vaccinia virus transcriptional promoter and integrated within the vaccinia virus genome. The recombinant vaccinia virus retained infectivity and stably expressed T7 RNA polymerase in mammalian cells. Target genes were constructed by inserting DNA segments that code for beta-galactosidase or chloramphenicol acetyltransferase into a plasmid with bacteriophage T7 promoter and terminator regions. When cells were infected with the recombinant vaccinia virus and transfected with plasmids containing the target genes, the latter were expressed at high levels. Chloramphenicol acetyltransferase activity was 400-600 times greater than that observed with conventional mammalian transient-expression systems regulated either by the enhancer and promoter regions of the Rous sarcoma virus long terminal repeat or by the simian virus 40 early region. The vaccinia/T7 hybrid virus forms the basis of a simple, rapid, widely applicable, and efficient mammalian expression system.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3095828

Citation

Fuerst, T R., et al. "Eukaryotic Transient-expression System Based On Recombinant Vaccinia Virus That Synthesizes Bacteriophage T7 RNA Polymerase." Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 21, 1986, pp. 8122-6.
Fuerst TR, Niles EG, Studier FW, et al. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci USA. 1986;83(21):8122-6.
Fuerst, T. R., Niles, E. G., Studier, F. W., & Moss, B. (1986). Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proceedings of the National Academy of Sciences of the United States of America, 83(21), 8122-6.
Fuerst TR, et al. Eukaryotic Transient-expression System Based On Recombinant Vaccinia Virus That Synthesizes Bacteriophage T7 RNA Polymerase. Proc Natl Acad Sci USA. 1986;83(21):8122-6. PubMed PMID: 3095828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. AU - Fuerst,T R, AU - Niles,E G, AU - Studier,F W, AU - Moss,B, PY - 1986/11/1/pubmed PY - 1986/11/1/medline PY - 1986/11/1/entrez SP - 8122 EP - 6 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 83 IS - 21 N2 - DNA coding for bacteriophage T7 RNA polymerase was ligated to a vaccinia virus transcriptional promoter and integrated within the vaccinia virus genome. The recombinant vaccinia virus retained infectivity and stably expressed T7 RNA polymerase in mammalian cells. Target genes were constructed by inserting DNA segments that code for beta-galactosidase or chloramphenicol acetyltransferase into a plasmid with bacteriophage T7 promoter and terminator regions. When cells were infected with the recombinant vaccinia virus and transfected with plasmids containing the target genes, the latter were expressed at high levels. Chloramphenicol acetyltransferase activity was 400-600 times greater than that observed with conventional mammalian transient-expression systems regulated either by the enhancer and promoter regions of the Rous sarcoma virus long terminal repeat or by the simian virus 40 early region. The vaccinia/T7 hybrid virus forms the basis of a simple, rapid, widely applicable, and efficient mammalian expression system. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/3095828/Eukaryotic_transient_expression_system_based_on_recombinant_vaccinia_virus_that_synthesizes_bacteriophage_T7_RNA_polymerase_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=3095828 DB - PRIME DP - Unbound Medicine ER -