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Fluorometric label-free aptasensor for detection of the pesticide acetamiprid by using cationic carbon dots prepared with cetrimonium bromide.
Mikrochim Acta. 2019 04 08; 186(5):273.MA

Abstract

A fluorometric aptamer-based method is described for sensitive detection of the pesticide acetamiprid. Cationic carbon dots (cCDs) with blue fluorescence were synthesized from cetrimonium bromide (CTAB) by a hydrothermal method. In the presence of the acetamiprid aptamers with a negative charge, the aptamers bind to the surface of the cCDs due to electrostatic attraction. As a result, the fluorescence of the cCDs is quenched partially (the best measurement was done at excitation/emission wavelengths of 360/445 nm). If acetamiprid is added to the above system, the aptamer binds to acetamiprid as a target with strong and specific affinity. Therefore, fluorescence increases proportionally to the acetamiprid concentrations. The aptasensor has a detection limit of 0.3 nM with a dynamic range from 1.6 to 120 nM which reveals that the method is sensitive in comparison to the other techniques. The selectivity of the method towards various pesticides was also studied and found to be adequate. The sensor was applied for the determination of acetamiprid in (spiked) wastewater, tap water, and tomatoes to underpin its practicability. Graphical abstract Cationic CDs (cCDs) were synthesized from cetrimonium bromide by a hydrothermal method. The addition of the negatively charged acetamiprid aptamer to a solution containing cCDs, the cCDs will be coated by the aptamer. This causes the blue fluorescence of the cCDs partially is quenched. If acetamiprid (ACP) is then added, the aptamer will bind to acetamiprid with strong and specific affinity. Hence, fluorescence will be gradually restored.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Saberi Z
Department of Chemistry, Isfahan University of Technology, Isfahan, I.R., 84156-83111, Iran.
Rezaei B
Department of Chemistry, Isfahan University of Technology, Isfahan, I.R., 84156-83111, Iran. rezaei@cc.iut.ac.ir.
Ensafi AA
Department of Chemistry, Isfahan University of Technology, Isfahan, I.R., 84156-83111, Iran.

MeSH

Analytic Sample Preparation MethodsAptamers, NucleotideBiosensing TechniquesCarbonCetrimoniumFluorometryLimit of DetectionSolanum lycopersicumModels, MolecularNanoparticlesNeonicotinoidsNucleic Acid ConformationPesticidesWater

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30963279

Citation

Saberi, Zeinab, et al. "Fluorometric Label-free Aptasensor for Detection of the Pesticide Acetamiprid By Using Cationic Carbon Dots Prepared With Cetrimonium Bromide." Mikrochimica Acta, vol. 186, no. 5, 2019, p. 273.
Saberi Z, Rezaei B, Ensafi AA. Fluorometric label-free aptasensor for detection of the pesticide acetamiprid by using cationic carbon dots prepared with cetrimonium bromide. Mikrochim Acta. 2019;186(5):273.
Saberi, Z., Rezaei, B., & Ensafi, A. A. (2019). Fluorometric label-free aptasensor for detection of the pesticide acetamiprid by using cationic carbon dots prepared with cetrimonium bromide. Mikrochimica Acta, 186(5), 273. https://doi.org/10.1007/s00604-019-3378-9
Saberi Z, Rezaei B, Ensafi AA. Fluorometric Label-free Aptasensor for Detection of the Pesticide Acetamiprid By Using Cationic Carbon Dots Prepared With Cetrimonium Bromide. Mikrochim Acta. 2019 04 8;186(5):273. PubMed PMID: 30963279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluorometric label-free aptasensor for detection of the pesticide acetamiprid by using cationic carbon dots prepared with cetrimonium bromide. AU - Saberi,Zeinab, AU - Rezaei,Behzad, AU - Ensafi,Ali Ashghar, Y1 - 2019/04/08/ PY - 2018/12/12/received PY - 2019/03/21/accepted PY - 2019/4/10/entrez PY - 2019/4/10/pubmed PY - 2020/3/5/medline KW - Acetamiprid KW - Aptamer KW - Carbon dots KW - Fluorescence KW - Nanoprobe SP - 273 EP - 273 JF - Mikrochimica acta JO - Mikrochim Acta VL - 186 IS - 5 N2 - A fluorometric aptamer-based method is described for sensitive detection of the pesticide acetamiprid. Cationic carbon dots (cCDs) with blue fluorescence were synthesized from cetrimonium bromide (CTAB) by a hydrothermal method. In the presence of the acetamiprid aptamers with a negative charge, the aptamers bind to the surface of the cCDs due to electrostatic attraction. As a result, the fluorescence of the cCDs is quenched partially (the best measurement was done at excitation/emission wavelengths of 360/445 nm). If acetamiprid is added to the above system, the aptamer binds to acetamiprid as a target with strong and specific affinity. Therefore, fluorescence increases proportionally to the acetamiprid concentrations. The aptasensor has a detection limit of 0.3 nM with a dynamic range from 1.6 to 120 nM which reveals that the method is sensitive in comparison to the other techniques. The selectivity of the method towards various pesticides was also studied and found to be adequate. The sensor was applied for the determination of acetamiprid in (spiked) wastewater, tap water, and tomatoes to underpin its practicability. Graphical abstract Cationic CDs (cCDs) were synthesized from cetrimonium bromide by a hydrothermal method. The addition of the negatively charged acetamiprid aptamer to a solution containing cCDs, the cCDs will be coated by the aptamer. This causes the blue fluorescence of the cCDs partially is quenched. If acetamiprid (ACP) is then added, the aptamer will bind to acetamiprid with strong and specific affinity. Hence, fluorescence will be gradually restored. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/30963279/Fluorometric_label_free_aptasensor_for_detection_of_the_pesticide_acetamiprid_by_using_cationic_carbon_dots_prepared_with_cetrimonium_bromide_ DB - PRIME DP - Unbound Medicine ER -