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DC Electric Fields Induce Perpendicular Alignment and Enhanced Migration in Schwann Cell Cultures.
Ann Biomed Eng 2019; 47(7):1584-1595AB

Abstract

Schwann cells (SCs) are PNS glia that play numerous support functions including myelination of axons. After PNS injury, SCs facilitate regeneration by phagocytosing cellular debris and providing physical and biochemical cues to guide axon growth. This reparative phenotype suggests SCs could be critical cellular targets for enhancing nerve regeneration. One method for altering cell morphology and motility is the application of direct current (DC) electric fields (EFs). Endogenous EFs have physiologic relevance during embryogenesis and serve as guidance and polarization cues. While much literature exists on EFs and CNS and PNS neurons, the effects of EFs on SCs have not been extensively studied. In this work, cell alignment, migration, and morphology of rat SCs were measured in response to several EF stimulation regimes including constant DC, 50% duty cycle DC and oscillating DC. SCs were found to re-orient perpendicular to field lines and respond to DC EFs as low as 75 mV/mm. EF exposure promoted directed migration, with travel towards the cathode at a mean rate of 7.5 µm/h. The data highlight the utility of EFs in modulating SC morphology, alignment and migration. Results may have implications for using EFs to attract and realign SCs at the site of PNS trauma.

Authors+Show Affiliations

Center for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA. Department of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.Center for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA. Department of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.Center for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA. li128@purdue.edu. Department of Basic Medical Sciences, Purdue University, West Lafayette, IN, 47907, USA. li128@purdue.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30963382

Citation

Bunn, Spencer J., et al. "DC Electric Fields Induce Perpendicular Alignment and Enhanced Migration in Schwann Cell Cultures." Annals of Biomedical Engineering, vol. 47, no. 7, 2019, pp. 1584-1595.
Bunn SJ, Lai A, Li J. DC Electric Fields Induce Perpendicular Alignment and Enhanced Migration in Schwann Cell Cultures. Ann Biomed Eng. 2019;47(7):1584-1595.
Bunn, S. J., Lai, A., & Li, J. (2019). DC Electric Fields Induce Perpendicular Alignment and Enhanced Migration in Schwann Cell Cultures. Annals of Biomedical Engineering, 47(7), pp. 1584-1595. doi:10.1007/s10439-019-02259-4.
Bunn SJ, Lai A, Li J. DC Electric Fields Induce Perpendicular Alignment and Enhanced Migration in Schwann Cell Cultures. Ann Biomed Eng. 2019;47(7):1584-1595. PubMed PMID: 30963382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DC Electric Fields Induce Perpendicular Alignment and Enhanced Migration in Schwann Cell Cultures. AU - Bunn,Spencer J, AU - Lai,Alexander, AU - Li,Jianming, Y1 - 2019/04/08/ PY - 2018/11/14/received PY - 2019/03/29/accepted PY - 2019/4/10/pubmed PY - 2019/4/10/medline PY - 2019/4/10/entrez KW - Bands of Bungner KW - Electrotaxis KW - Galvanotaxis KW - Peripheral nerve repair KW - Voltage gradient SP - 1584 EP - 1595 JF - Annals of biomedical engineering JO - Ann Biomed Eng VL - 47 IS - 7 N2 - Schwann cells (SCs) are PNS glia that play numerous support functions including myelination of axons. After PNS injury, SCs facilitate regeneration by phagocytosing cellular debris and providing physical and biochemical cues to guide axon growth. This reparative phenotype suggests SCs could be critical cellular targets for enhancing nerve regeneration. One method for altering cell morphology and motility is the application of direct current (DC) electric fields (EFs). Endogenous EFs have physiologic relevance during embryogenesis and serve as guidance and polarization cues. While much literature exists on EFs and CNS and PNS neurons, the effects of EFs on SCs have not been extensively studied. In this work, cell alignment, migration, and morphology of rat SCs were measured in response to several EF stimulation regimes including constant DC, 50% duty cycle DC and oscillating DC. SCs were found to re-orient perpendicular to field lines and respond to DC EFs as low as 75 mV/mm. EF exposure promoted directed migration, with travel towards the cathode at a mean rate of 7.5 µm/h. The data highlight the utility of EFs in modulating SC morphology, alignment and migration. Results may have implications for using EFs to attract and realign SCs at the site of PNS trauma. SN - 1573-9686 UR - https://www.unboundmedicine.com/medline/citation/30963382/DC_Electric_Fields_Induce_Perpendicular_Alignment_and_Enhanced_Migration_in_Schwann_Cell_Cultures L2 - https://doi.org/10.1007/s10439-019-02259-4 DB - PRIME DP - Unbound Medicine ER -