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Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma by Weighted Gene Co-Expression Network Analysis.
Med Sci Monit. 2019 Apr 09; 25:2609-2622.MS

Abstract

BACKGROUND

More and more recent studies have clearly shown that long non-coding RNA (lncRNA) should be considered as a fundamental part of the ceRNA network, mainly because lncRNA can act as miRNA sponges to regulate the protein-coding gene expression. Nevertheless, it is still not clear how lncRNA-mediated ceRNAs function in cervical squamous cell carcinoma (CESC). Moreover, information about the ceRNA regulatory mechanism is also remarkably limited; thus, prediction of CESC prognosis using ceRNA-related information remains challenging. MATERIAL AND

METHODS

We collected 306 RNA (lncRNA, miRNA, and mRNA) expression profile datasets obtained from cervical squamous cancer tissues plus 3 more from adjacent cervical tissues via the TCGA database. Subsequently, we constructed a lncRNAs-miRNAs-mRNAs CESC ceRNA network, and Gene Ontology (GO) analysis was carried out.

RESULTS

We identified a total of 30 DElncRNAs, 70 DEmiRNAs, and 1089 DEmRNAs in CESC. Subsequently, to reveal the expression patterns of dysregulated genes, weighted gene co-expression network analysis was carried out, resulting in 3 co-expression modules with significantly related clinical properties. The constructed aberrant lncRNAs-miRNAs-mRNAs CESC ceRNA network was composed of 17 DEmiRNAs, 5 DElncRNAs, and 7 DEmRNAs. Moreover, the survival analysis was performed for DElncRNAs, DEmiRNAs, and DEmRNAs.

CONCLUSIONS

The present study shows the involvement of the lncRNA-related ceRNA network in the pathogenesis of CESC. We believe the newly generated ceRNA network will provide more insights into the lncRNA-mediated ceRNA regulatory mechanisms.

Authors+Show Affiliations

College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China (mainland).College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China (mainland).College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China (mainland).College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China (mainland).College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China (mainland). Jiangmen Wuyi Traditional Chinese Medicine Hospital, Jiangmen, Guangdong, China (mainland).College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China (mainland).College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China (mainland).

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30965350

Citation

Chen, Weihao, et al. "Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma By Weighted Gene Co-Expression Network Analysis." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 25, 2019, pp. 2609-2622.
Chen W, Chen X, Wang Y, et al. Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma by Weighted Gene Co-Expression Network Analysis. Med Sci Monit. 2019;25:2609-2622.
Chen, W., Chen, X., Wang, Y., Liu, T., Liang, Y., Xiao, Y., & Chen, L. (2019). Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma by Weighted Gene Co-Expression Network Analysis. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 2609-2622. https://doi.org/10.12659/MSM.913471
Chen W, et al. Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma By Weighted Gene Co-Expression Network Analysis. Med Sci Monit. 2019 Apr 9;25:2609-2622. PubMed PMID: 30965350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma by Weighted Gene Co-Expression Network Analysis. AU - Chen,Weihao, AU - Chen,Xudong, AU - Wang,Yurong, AU - Liu,Tianhao, AU - Liang,Yudan, AU - Xiao,Ya, AU - Chen,Liguo, Y1 - 2019/04/09/ PY - 2019/4/10/entrez PY - 2019/4/10/pubmed PY - 2019/8/3/medline SP - 2609 EP - 2622 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med. Sci. Monit. VL - 25 N2 - BACKGROUND More and more recent studies have clearly shown that long non-coding RNA (lncRNA) should be considered as a fundamental part of the ceRNA network, mainly because lncRNA can act as miRNA sponges to regulate the protein-coding gene expression. Nevertheless, it is still not clear how lncRNA-mediated ceRNAs function in cervical squamous cell carcinoma (CESC). Moreover, information about the ceRNA regulatory mechanism is also remarkably limited; thus, prediction of CESC prognosis using ceRNA-related information remains challenging. MATERIAL AND METHODS We collected 306 RNA (lncRNA, miRNA, and mRNA) expression profile datasets obtained from cervical squamous cancer tissues plus 3 more from adjacent cervical tissues via the TCGA database. Subsequently, we constructed a lncRNAs-miRNAs-mRNAs CESC ceRNA network, and Gene Ontology (GO) analysis was carried out. RESULTS We identified a total of 30 DElncRNAs, 70 DEmiRNAs, and 1089 DEmRNAs in CESC. Subsequently, to reveal the expression patterns of dysregulated genes, weighted gene co-expression network analysis was carried out, resulting in 3 co-expression modules with significantly related clinical properties. The constructed aberrant lncRNAs-miRNAs-mRNAs CESC ceRNA network was composed of 17 DEmiRNAs, 5 DElncRNAs, and 7 DEmRNAs. Moreover, the survival analysis was performed for DElncRNAs, DEmiRNAs, and DEmRNAs. CONCLUSIONS The present study shows the involvement of the lncRNA-related ceRNA network in the pathogenesis of CESC. We believe the newly generated ceRNA network will provide more insights into the lncRNA-mediated ceRNA regulatory mechanisms. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/30965350/Construction_and_Analysis_of_lncRNA_Mediated_ceRNA_Network_in_Cervical_Squamous_Cell_Carcinoma_by_Weighted_Gene_Co_Expression_Network_Analysis_ L2 - https://www.medscimonit.com/download/index/idArt/913471 DB - PRIME DP - Unbound Medicine ER -