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Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma by Weighted Gene Co-Expression Network Analysis.Med Sci Monit. 2019 Apr 09; 25:2609-2622.MS
Abstract
BACKGROUND
More and more recent studies have clearly shown that long non-coding RNA (lncRNA) should be considered as a fundamental part of the ceRNA network, mainly because lncRNA can act as miRNA sponges to regulate the protein-coding gene expression. Nevertheless, it is still not clear how lncRNA-mediated ceRNAs function in cervical squamous cell carcinoma (CESC). Moreover, information about the ceRNA regulatory mechanism is also remarkably limited; thus, prediction of CESC prognosis using ceRNA-related information remains challenging. MATERIAL ANDMETHODS
We collected 306 RNA (lncRNA, miRNA, and mRNA) expression profile datasets obtained from cervical squamous cancer tissues plus 3 more from adjacent cervical tissues via the TCGA database. Subsequently, we constructed a lncRNAs-miRNAs-mRNAs CESC ceRNA network, and Gene Ontology (GO) analysis was carried out.RESULTS
We identified a total of 30 DElncRNAs, 70 DEmiRNAs, and 1089 DEmRNAs in CESC. Subsequently, to reveal the expression patterns of dysregulated genes, weighted gene co-expression network analysis was carried out, resulting in 3 co-expression modules with significantly related clinical properties. The constructed aberrant lncRNAs-miRNAs-mRNAs CESC ceRNA network was composed of 17 DEmiRNAs, 5 DElncRNAs, and 7 DEmRNAs. Moreover, the survival analysis was performed for DElncRNAs, DEmiRNAs, and DEmRNAs.CONCLUSIONS
The present study shows the involvement of the lncRNA-related ceRNA network in the pathogenesis of CESC. We believe the newly generated ceRNA network will provide more insights into the lncRNA-mediated ceRNA regulatory mechanisms.Links
MeSH
Pub Type(s)
Journal Article
Language
eng
PubMed ID
30965350
Citation
Chen, Weihao, et al. "Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma By Weighted Gene Co-Expression Network Analysis." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 25, 2019, pp. 2609-2622.
Chen W, Chen X, Wang Y, et al. Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma by Weighted Gene Co-Expression Network Analysis. Med Sci Monit. 2019;25:2609-2622.
Chen, W., Chen, X., Wang, Y., Liu, T., Liang, Y., Xiao, Y., & Chen, L. (2019). Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma by Weighted Gene Co-Expression Network Analysis. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 2609-2622. https://doi.org/10.12659/MSM.913471
Chen W, et al. Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma By Weighted Gene Co-Expression Network Analysis. Med Sci Monit. 2019 Apr 9;25:2609-2622. PubMed PMID: 30965350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Construction and Analysis of lncRNA-Mediated ceRNA Network in Cervical Squamous Cell Carcinoma by Weighted Gene Co-Expression Network Analysis.
AU - Chen,Weihao,
AU - Chen,Xudong,
AU - Wang,Yurong,
AU - Liu,Tianhao,
AU - Liang,Yudan,
AU - Xiao,Ya,
AU - Chen,Liguo,
Y1 - 2019/04/09/
PY - 2019/4/10/entrez
PY - 2019/4/10/pubmed
PY - 2019/8/3/medline
SP - 2609
EP - 2622
JF - Medical science monitor : international medical journal of experimental and clinical research
JO - Med Sci Monit
VL - 25
N2 - BACKGROUND More and more recent studies have clearly shown that long non-coding RNA (lncRNA) should be considered as a fundamental part of the ceRNA network, mainly because lncRNA can act as miRNA sponges to regulate the protein-coding gene expression. Nevertheless, it is still not clear how lncRNA-mediated ceRNAs function in cervical squamous cell carcinoma (CESC). Moreover, information about the ceRNA regulatory mechanism is also remarkably limited; thus, prediction of CESC prognosis using ceRNA-related information remains challenging. MATERIAL AND METHODS We collected 306 RNA (lncRNA, miRNA, and mRNA) expression profile datasets obtained from cervical squamous cancer tissues plus 3 more from adjacent cervical tissues via the TCGA database. Subsequently, we constructed a lncRNAs-miRNAs-mRNAs CESC ceRNA network, and Gene Ontology (GO) analysis was carried out. RESULTS We identified a total of 30 DElncRNAs, 70 DEmiRNAs, and 1089 DEmRNAs in CESC. Subsequently, to reveal the expression patterns of dysregulated genes, weighted gene co-expression network analysis was carried out, resulting in 3 co-expression modules with significantly related clinical properties. The constructed aberrant lncRNAs-miRNAs-mRNAs CESC ceRNA network was composed of 17 DEmiRNAs, 5 DElncRNAs, and 7 DEmRNAs. Moreover, the survival analysis was performed for DElncRNAs, DEmiRNAs, and DEmRNAs. CONCLUSIONS The present study shows the involvement of the lncRNA-related ceRNA network in the pathogenesis of CESC. We believe the newly generated ceRNA network will provide more insights into the lncRNA-mediated ceRNA regulatory mechanisms.
SN - 1643-3750
UR - https://www.unboundmedicine.com/medline/citation/30965350/Construction_and_Analysis_of_lncRNA_Mediated_ceRNA_Network_in_Cervical_Squamous_Cell_Carcinoma_by_Weighted_Gene_Co_Expression_Network_Analysis_
L2 - https://www.medscimonit.com/download/index/idArt/913471
DB - PRIME
DP - Unbound Medicine
ER -