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Novel data from Italian Vermamoeba vermiformis isolates from multiple sources add to genetic diversity within the genus.
Parasitol Res. 2019 Jun; 118(6):1751-1759.PR

Abstract

Vermamoeba vermiformis represents one of the most common free-living amoebae identified in worldwide environmental surveys. We analyzed 56 water samples with varying characteristics, including temperature and the particular settings in which humans may be exposed to water, plus one corneal scraping from a keratitis patient, with the following aims: (i) to investigate the presence of V. vermiformis; (ii) to identify the isolate subtypes; (iii) to place the Italian isolates in the broader picture of the genetic diversity within V. vermiformis. Twenty-two isolates were identified upon culturing and sequencing of > 600 bp in the 18S ribosomal RNA (rRNA) gene sequence, bringing to 27 the number of sequences recovered from Italian sources. By adding deposited sequences, we assembled a dataset of 74 isolates. Three of our isolates were characterized by allelic code 7-5-1-1, never reported before, and two showed 100% identity with an uncultured eukaryote and carried the 719T>C variant. We show that the variable segments E5, E3, F, and G convey most of the information on diversity, enabling the clustering of the isolates in a replicable fashion. The presence of different strains in natural thermal waters and in distribution systems indicated heterogeneity of the amoebic populations. Also, ours and the only other sequence from human infection were mapped in different clades. Overall, we enlarged the repertoire of single nucleotide and indel variants and the list of allelic codes, proceeding one step further in the description of the diversity within the genus.

Authors+Show Affiliations

Department of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, Rome, Italy.Department of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, Rome, Italy. berrilli@uniroma2.it.Department of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, Rome, Italy.Department of Biology, University of Rome Tor Vergata, Rome, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30968262

Citation

Montalbano Di Filippo, M, et al. "Novel Data From Italian Vermamoeba Vermiformis Isolates From Multiple Sources Add to Genetic Diversity Within the Genus." Parasitology Research, vol. 118, no. 6, 2019, pp. 1751-1759.
Montalbano Di Filippo M, Berrilli F, Di Cave D, et al. Novel data from Italian Vermamoeba vermiformis isolates from multiple sources add to genetic diversity within the genus. Parasitol Res. 2019;118(6):1751-1759.
Montalbano Di Filippo, M., Berrilli, F., Di Cave, D., & Novelletto, A. (2019). Novel data from Italian Vermamoeba vermiformis isolates from multiple sources add to genetic diversity within the genus. Parasitology Research, 118(6), 1751-1759. https://doi.org/10.1007/s00436-019-06294-x
Montalbano Di Filippo M, et al. Novel Data From Italian Vermamoeba Vermiformis Isolates From Multiple Sources Add to Genetic Diversity Within the Genus. Parasitol Res. 2019;118(6):1751-1759. PubMed PMID: 30968262.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel data from Italian Vermamoeba vermiformis isolates from multiple sources add to genetic diversity within the genus. AU - Montalbano Di Filippo,M, AU - Berrilli,F, AU - Di Cave,D, AU - Novelletto,A, Y1 - 2019/04/09/ PY - 2018/11/09/received PY - 2019/03/14/accepted PY - 2019/4/11/pubmed PY - 2019/8/14/medline PY - 2019/4/11/entrez KW - 18S rRNA KW - Allelic variants KW - Amoebozoa KW - Dynamic homology KW - Vermamoeba vermiformis SP - 1751 EP - 1759 JF - Parasitology research JO - Parasitol Res VL - 118 IS - 6 N2 - Vermamoeba vermiformis represents one of the most common free-living amoebae identified in worldwide environmental surveys. We analyzed 56 water samples with varying characteristics, including temperature and the particular settings in which humans may be exposed to water, plus one corneal scraping from a keratitis patient, with the following aims: (i) to investigate the presence of V. vermiformis; (ii) to identify the isolate subtypes; (iii) to place the Italian isolates in the broader picture of the genetic diversity within V. vermiformis. Twenty-two isolates were identified upon culturing and sequencing of > 600 bp in the 18S ribosomal RNA (rRNA) gene sequence, bringing to 27 the number of sequences recovered from Italian sources. By adding deposited sequences, we assembled a dataset of 74 isolates. Three of our isolates were characterized by allelic code 7-5-1-1, never reported before, and two showed 100% identity with an uncultured eukaryote and carried the 719T>C variant. We show that the variable segments E5, E3, F, and G convey most of the information on diversity, enabling the clustering of the isolates in a replicable fashion. The presence of different strains in natural thermal waters and in distribution systems indicated heterogeneity of the amoebic populations. Also, ours and the only other sequence from human infection were mapped in different clades. Overall, we enlarged the repertoire of single nucleotide and indel variants and the list of allelic codes, proceeding one step further in the description of the diversity within the genus. SN - 1432-1955 UR - https://www.unboundmedicine.com/medline/citation/30968262/Novel_data_from_Italian_Vermamoeba_vermiformis_isolates_from_multiple_sources_add_to_genetic_diversity_within_the_genus_ L2 - https://dx.doi.org/10.1007/s00436-019-06294-x DB - PRIME DP - Unbound Medicine ER -