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Early α-linolenic acid exposure to embryo reduces pentylenetetrazol-induced seizures in zebrafish larva.

Abstract

Over the past few years, there has been a tremendous increase in interest of general population toward food-based therapies for management of chronic clinical conditions due to their lesser adverse effects with prolonged use over pharmacotherapies. Foods enriched with omega-3 fatty acids have shown some promising results in case of epilepsy. The present study was envisioned to investigate the effect of early exposure of α-linolenic acid (ALA), an essential omega-3 fatty acid in developing zebrafish (Danio rerio) embryos toward pentylenetetrazol (PTZ)-induced seizure susceptibility. The healthy wild-type zebrafish embryos were incubated in system water or system water containing different ALA concentrations (1-20 µM) till 7 dpf (days post fertilization). Each larva at 7 dpf was placed in 8 mM PTZ solution and seizure event was recorded. ALA incubation at 10 µM and 20 µM concentrations showed a dose-dependent reduction in PTZ-mediated hyperactive responses in larvae indicated by a marked decrease in total distance travelled and speed, as compared to vehicle control. Furthermore, both the treated groups showed increase in the latency to PTZ-induced clonus-like seizures in larvae, as compared to vehicle control. ALA incubated larvae at 10 µM and 20 µM concentrations also showed a significant reduction in c-fos mRNA level. A marked increase in the level of ALA and docosahexaenoic acid was also observed in the larvae incubated at highest effective concentration of ALA. The present study concluded that embryonic exposure of ALA reduced PTZ-induced seizures in zebrafish larva.

Authors+Show Affiliations

Pharmacology and Toxicology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India.Pharmacology and Toxicology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India.Pharmacology and Toxicology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India.Pharmacology and Toxicology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India. Electronic address: damanpreet@ihbt.res.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30975378

Citation

Kumari, Savita, et al. "Early Α-linolenic Acid Exposure to Embryo Reduces Pentylenetetrazol-induced Seizures in Zebrafish Larva." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 143, 2019, pp. 15-20.
Kumari S, Mazumder AG, Bhardwaj A, et al. Early α-linolenic acid exposure to embryo reduces pentylenetetrazol-induced seizures in zebrafish larva. Prostaglandins Leukot Essent Fatty Acids. 2019;143:15-20.
Kumari, S., Mazumder, A. G., Bhardwaj, A., & Singh, D. (2019). Early α-linolenic acid exposure to embryo reduces pentylenetetrazol-induced seizures in zebrafish larva. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 143, pp. 15-20. doi:10.1016/j.plefa.2019.02.002.
Kumari S, et al. Early Α-linolenic Acid Exposure to Embryo Reduces Pentylenetetrazol-induced Seizures in Zebrafish Larva. Prostaglandins Leukot Essent Fatty Acids. 2019;143:15-20. PubMed PMID: 30975378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Early α-linolenic acid exposure to embryo reduces pentylenetetrazol-induced seizures in zebrafish larva. AU - Kumari,Savita, AU - Mazumder,Arindam Ghosh, AU - Bhardwaj,Avantika, AU - Singh,Damanpreet, Y1 - 2019/03/01/ PY - 2019/02/27/received PY - 2019/02/28/accepted PY - 2019/4/13/entrez KW - Clonus-like seizures KW - Omega-3 fatty acids KW - Pentylenetetrazol KW - Polyunsaturated fatty acids KW - Zebrafish KW - c-fos SP - 15 EP - 20 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot. Essent. Fatty Acids VL - 143 N2 - Over the past few years, there has been a tremendous increase in interest of general population toward food-based therapies for management of chronic clinical conditions due to their lesser adverse effects with prolonged use over pharmacotherapies. Foods enriched with omega-3 fatty acids have shown some promising results in case of epilepsy. The present study was envisioned to investigate the effect of early exposure of α-linolenic acid (ALA), an essential omega-3 fatty acid in developing zebrafish (Danio rerio) embryos toward pentylenetetrazol (PTZ)-induced seizure susceptibility. The healthy wild-type zebrafish embryos were incubated in system water or system water containing different ALA concentrations (1-20 µM) till 7 dpf (days post fertilization). Each larva at 7 dpf was placed in 8 mM PTZ solution and seizure event was recorded. ALA incubation at 10 µM and 20 µM concentrations showed a dose-dependent reduction in PTZ-mediated hyperactive responses in larvae indicated by a marked decrease in total distance travelled and speed, as compared to vehicle control. Furthermore, both the treated groups showed increase in the latency to PTZ-induced clonus-like seizures in larvae, as compared to vehicle control. ALA incubated larvae at 10 µM and 20 µM concentrations also showed a significant reduction in c-fos mRNA level. A marked increase in the level of ALA and docosahexaenoic acid was also observed in the larvae incubated at highest effective concentration of ALA. The present study concluded that embryonic exposure of ALA reduced PTZ-induced seizures in zebrafish larva. SN - 1532-2823 UR - https://www.unboundmedicine.com/medline/citation/30975378/Early_α-linolenic_acid_exposure_to_embryo_reduces_pentylenetetrazol-induced_seizures_in_zebrafish_larva L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(19)30049-3 DB - PRIME DP - Unbound Medicine ER -