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Effects of ATRAP in Renal Proximal Tubules on Angiotensin-Dependent Hypertension.
J Am Heart Assoc. 2019 04 16; 8(8):e012395.JA

Abstract

Background We have previously shown that ATRAP (angiotensin II receptor-associated protein; Agtrap) interacts with AT1R (angiotensin II type 1 receptor) and promotes constitutive internalization of AT 1R so as to inhibit hyperactivation of its downstream signaling. In response to angiotensin II , systemic ATRAP deficiency exacerbates angiotensin II -mediated hypertension via hyperactivation of renal tubular AT 1R. Although ATRAP expression is abundant in renal proximal tubules, little is known about the actual function of renal proximal tubule ATRAP in angiotensin-mediated hypertension. Methods and Results In this study, we examined the in vivo functional role of renal proximal tubule ATRAP in angiotensin-dependent hypertension. We succeeded in generating proximal tubule-specific ATRAP knockout (PT - KO) mice for the first time using the Cre/loxP system with Pepck-Cre. Detailed analysis of renal ATRAP expression in PT - KO mice estimated by immunohistochemical and laser-capture microdissection analysis revealed that ATRAP mRNA expression decreased by ≈80% in proximal regions of the nephron in PT - KO mice compared with wild-type (WT) mice. We compared blood pressure of PT - KO and WT mice using both tail-cuff and radiotelemetric methods. Blood pressure of PT - KO mice was comparable with that of WT mice at baseline. Moreover, no significant differences were noted in pressor response to angiotensin II (600 ng/kg per min or 1000 ng/kg per minute) infusion between PT - KO and WT mice. In addition, angiotensin II -mediated cardiac hypertrophy was identical between PT - KO and WT mice. Conclusions ATRAP deficiency in proximal tubules did not exacerbate angiotensin-dependent hypertension in vivo. The results indicate that renal proximal tubule ATRAP has a minor role in angiotensin-dependent hypertension in vivo.

Authors+Show Affiliations

1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan. 2 Cardiovascular and Metabolic Disorders Program Duke-NUS Medical School Singapore Singapore.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.3 Department of Molecular Biology Yokohama City University Graduate School of Medicine Yokohama Japan.4 Center for Health Service Sciences Yokohama National University Yokohama Japan.1 Department of Medical Science and Cardiorenal Medicine Yokohama City University Graduate School of Medicine Yokohama Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30977419

Citation

Kinguchi, Sho, et al. "Effects of ATRAP in Renal Proximal Tubules On Angiotensin-Dependent Hypertension." Journal of the American Heart Association, vol. 8, no. 8, 2019, pp. e012395.
Kinguchi S, Wakui H, Azushima K, et al. Effects of ATRAP in Renal Proximal Tubules on Angiotensin-Dependent Hypertension. J Am Heart Assoc. 2019;8(8):e012395.
Kinguchi, S., Wakui, H., Azushima, K., Haruhara, K., Koguchi, T., Ohki, K., Uneda, K., Matsuda, M., Haku, S., Yamaji, T., Yamada, T., Kobayashi, R., Minegishi, S., Ishigami, T., Yamashita, A., Fujikawa, T., & Tamura, K. (2019). Effects of ATRAP in Renal Proximal Tubules on Angiotensin-Dependent Hypertension. Journal of the American Heart Association, 8(8), e012395. https://doi.org/10.1161/JAHA.119.012395
Kinguchi S, et al. Effects of ATRAP in Renal Proximal Tubules On Angiotensin-Dependent Hypertension. J Am Heart Assoc. 2019 04 16;8(8):e012395. PubMed PMID: 30977419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of ATRAP in Renal Proximal Tubules on Angiotensin-Dependent Hypertension. AU - Kinguchi,Sho, AU - Wakui,Hiromichi, AU - Azushima,Kengo, AU - Haruhara,Kotaro, AU - Koguchi,Tomoyuki, AU - Ohki,Kohji, AU - Uneda,Kazushi, AU - Matsuda,Miyuki, AU - Haku,Sona, AU - Yamaji,Takahiro, AU - Yamada,Takayuki, AU - Kobayashi,Ryu, AU - Minegishi,Shintaro, AU - Ishigami,Tomoaki, AU - Yamashita,Akio, AU - Fujikawa,Tetsuya, AU - Tamura,Kouichi, PY - 2019/4/13/entrez PY - 2019/4/13/pubmed PY - 2020/7/24/medline KW - hypertension KW - kidney KW - renin–angiotensin system SP - e012395 EP - e012395 JF - Journal of the American Heart Association JO - J Am Heart Assoc VL - 8 IS - 8 N2 - Background We have previously shown that ATRAP (angiotensin II receptor-associated protein; Agtrap) interacts with AT1R (angiotensin II type 1 receptor) and promotes constitutive internalization of AT 1R so as to inhibit hyperactivation of its downstream signaling. In response to angiotensin II , systemic ATRAP deficiency exacerbates angiotensin II -mediated hypertension via hyperactivation of renal tubular AT 1R. Although ATRAP expression is abundant in renal proximal tubules, little is known about the actual function of renal proximal tubule ATRAP in angiotensin-mediated hypertension. Methods and Results In this study, we examined the in vivo functional role of renal proximal tubule ATRAP in angiotensin-dependent hypertension. We succeeded in generating proximal tubule-specific ATRAP knockout (PT - KO) mice for the first time using the Cre/loxP system with Pepck-Cre. Detailed analysis of renal ATRAP expression in PT - KO mice estimated by immunohistochemical and laser-capture microdissection analysis revealed that ATRAP mRNA expression decreased by ≈80% in proximal regions of the nephron in PT - KO mice compared with wild-type (WT) mice. We compared blood pressure of PT - KO and WT mice using both tail-cuff and radiotelemetric methods. Blood pressure of PT - KO mice was comparable with that of WT mice at baseline. Moreover, no significant differences were noted in pressor response to angiotensin II (600 ng/kg per min or 1000 ng/kg per minute) infusion between PT - KO and WT mice. In addition, angiotensin II -mediated cardiac hypertrophy was identical between PT - KO and WT mice. Conclusions ATRAP deficiency in proximal tubules did not exacerbate angiotensin-dependent hypertension in vivo. The results indicate that renal proximal tubule ATRAP has a minor role in angiotensin-dependent hypertension in vivo. SN - 2047-9980 UR - https://www.unboundmedicine.com/medline/citation/30977419/Effects_of_ATRAP_in_Renal_Proximal_Tubules_on_Angiotensin_Dependent_Hypertension_ L2 - https://www.ahajournals.org/doi/10.1161/JAHA.119.012395?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -