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Microarray profiling of lung long non-coding RNAs and mRNAs in lipopolysaccharide-induced acute lung injury mouse model.
Biosci Rep. 2019 04 30; 39(4)BR

Abstract

Long non-coding RNAs (lncRNAs) are involved in various biological processes as well as many respiratory diseases, while the role of lncRNAs in acute lung injury (ALI) remains unclear. The present study aimed to profile the expression of lung lncRNAs and mRNAs in lipopolysaccharide (LPS)-induced ALI mouse model. C57BL/6 mice were exposed to LPS or phosphate-buffered saline for 24 h, and lncRNAs and mRNAs were profiled by Arraystar mouse LncRNA Array V3.0. Bioinformatics analysis gene ontology including (GO) and pathway analysis and cell study in vitro was used to investigate potential mechanisms. Based on the microarray results, 2632 lncRNAs and 2352 mRNAs were differentially expressed between ALI and control mice. The microarray results were confirmed by the quantitative real-time PCR (qRT-PCR) results of ten randomized selected lncRNAs. GO analysis showed that the altered mRNAs were mainly related to the processes of immune system, immune response and defense response. Pathway analysis suggests that tumor necrosis factor (TNF) signaling pathway, NOD-like receptor pathway, and cytokine-cytokine receptor interaction may be involved in ALI. LncRNA-mRNA co-expression network analysis indicated that one individual lncRNA may interact with several mRNAs, and one individual mRNA may also interact with several lncRNAs. Small interfering RNA (siRNA) for ENSMUST00000170214.1, - ENSMUST00000016031.13 significantly inhibited LPS-induced TNF-α and interleukin (IL)-1β production in murine RAW264.7 macrophages. Our results found significant changes of lncRNAs and mRNAs in the lungs of LPS-induced ALI mouse model, and intervention targeting lncRNAs may attenuate LPS-induced inflammation, which may help to elucidate the role of lncRNAs in the pathogenesis and treatment of ALI.

Authors+Show Affiliations

Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu 610041, China.Department of Respiratory and Critical Care Medicine, West China Hospital of Sichuan University, and Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Chengdu 610041, China.Department of Medical Affairs, West China Hospital of Sichuan University, Chengdu 610041, China.Department of Respiratory and Critical Care Medicine, West China Hospital of Sichuan University, and Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Chengdu 610041, China.Department of Respiratory and Critical Care Medicine, West China Hospital of Sichuan University, and Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Chengdu 610041, China.Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu 610041, China liubusiness@163.com lidajiang@cd120.com lidajiang@wchscu.cn. Department of Medical Affairs, West China Hospital of Sichuan University, Chengdu 610041, China.Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu 610041, China liubusiness@163.com lidajiang@cd120.com lidajiang@wchscu.cn. Department of Medical Affairs, West China Hospital of Sichuan University, Chengdu 610041, China.Department of Respiratory and Critical Care Medicine, West China Hospital of Sichuan University, and Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Chengdu 610041, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30979832

Citation

Wang, Juan, et al. "Microarray Profiling of Lung Long Non-coding RNAs and mRNAs in Lipopolysaccharide-induced Acute Lung Injury Mouse Model." Bioscience Reports, vol. 39, no. 4, 2019.
Wang J, Shen YC, Chen ZN, et al. Microarray profiling of lung long non-coding RNAs and mRNAs in lipopolysaccharide-induced acute lung injury mouse model. Biosci Rep. 2019;39(4).
Wang, J., Shen, Y. C., Chen, Z. N., Yuan, Z. C., Wang, H., Li, D. J., Liu, K., & Wen, F. Q. (2019). Microarray profiling of lung long non-coding RNAs and mRNAs in lipopolysaccharide-induced acute lung injury mouse model. Bioscience Reports, 39(4). https://doi.org/10.1042/BSR20181634
Wang J, et al. Microarray Profiling of Lung Long Non-coding RNAs and mRNAs in Lipopolysaccharide-induced Acute Lung Injury Mouse Model. Biosci Rep. 2019 04 30;39(4) PubMed PMID: 30979832.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microarray profiling of lung long non-coding RNAs and mRNAs in lipopolysaccharide-induced acute lung injury mouse model. AU - Wang,Juan, AU - Shen,Yong-Chun, AU - Chen,Zhen-Ni, AU - Yuan,Zhi-Cheng, AU - Wang,Hao, AU - Li,Da-Jiang, AU - Liu,Kai, AU - Wen,Fu-Qiang, Y1 - 2019/04/30/ PY - 2018/09/16/received PY - 2019/04/03/revised PY - 2019/04/10/accepted PY - 2019/4/14/pubmed PY - 2019/4/14/medline PY - 2019/4/14/entrez KW - Acute lung injury KW - Lipopolysaccharide KW - Long noncoding RNAs KW - Microarray analysis JF - Bioscience reports JO - Biosci. Rep. VL - 39 IS - 4 N2 - Long non-coding RNAs (lncRNAs) are involved in various biological processes as well as many respiratory diseases, while the role of lncRNAs in acute lung injury (ALI) remains unclear. The present study aimed to profile the expression of lung lncRNAs and mRNAs in lipopolysaccharide (LPS)-induced ALI mouse model. C57BL/6 mice were exposed to LPS or phosphate-buffered saline for 24 h, and lncRNAs and mRNAs were profiled by Arraystar mouse LncRNA Array V3.0. Bioinformatics analysis gene ontology including (GO) and pathway analysis and cell study in vitro was used to investigate potential mechanisms. Based on the microarray results, 2632 lncRNAs and 2352 mRNAs were differentially expressed between ALI and control mice. The microarray results were confirmed by the quantitative real-time PCR (qRT-PCR) results of ten randomized selected lncRNAs. GO analysis showed that the altered mRNAs were mainly related to the processes of immune system, immune response and defense response. Pathway analysis suggests that tumor necrosis factor (TNF) signaling pathway, NOD-like receptor pathway, and cytokine-cytokine receptor interaction may be involved in ALI. LncRNA-mRNA co-expression network analysis indicated that one individual lncRNA may interact with several mRNAs, and one individual mRNA may also interact with several lncRNAs. Small interfering RNA (siRNA) for ENSMUST00000170214.1, - ENSMUST00000016031.13 significantly inhibited LPS-induced TNF-α and interleukin (IL)-1β production in murine RAW264.7 macrophages. Our results found significant changes of lncRNAs and mRNAs in the lungs of LPS-induced ALI mouse model, and intervention targeting lncRNAs may attenuate LPS-induced inflammation, which may help to elucidate the role of lncRNAs in the pathogenesis and treatment of ALI. SN - 1573-4935 UR - https://www.unboundmedicine.com/medline/citation/30979832/Microarray_profiling_of_lung_long_non_coding_RNAs_and_mRNAs_in_lipopolysaccharide_induced_acute_lung_injury_mouse_model_ L2 - https://portlandpress.com/bioscirep/article-lookup/doi/10.1042/BSR20181634 DB - PRIME DP - Unbound Medicine ER -
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