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Myocardial protection by propolis during prolonged hypothermic preservation.
Cryobiology. 2019 06; 88:29-37.C

Abstract

Oxidative stress is involved in the pathogenesis of ischemia-reperfusion during myocardial transplantation. Therefore, graft preservation solutions may be improved by supplementation with antioxidants to minimize graft dysfunction caused by cold ischemic injury. Propolis is a polyphenol-rich substance which has an important antioxidant activity. The protective effect of propolis against oxidative stress induced by prolonged cold preservation of heart was investigated. Mice were subjected to a hypothermic model of ischemia in which hearts were preserved for 24 h at 4 °C in Krebs-Hensleit (KH) solution in the absence or presence of propolis concentrations (50, 150 and 250 μg/ml). Levels of released Lactate dehydrogenase (LDH), Creatine phosphokinase (CPK) and Troponine-I (Trop I) were assessed in the preservation solution and histological assessement of heart ischemia injuries was performed. Oxidative stress biomarkers malondialdehyde (MDA) and advanced oxidation protein products (AOPP) and antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) were assessed in cardiac tissue. Mitochondria were isolated from stored hearts and production of reactive oxygen species (ROS) was tested. Propolis supplementation protected efficiently hearts during preservation by reducing significantly levels of lipids and proteins oxidation and restoring activities of antioxidant enzymes. Also, propolis preserved tissue integrity altered by hypothermic ischemia in a concentration-dependent manner. Propolis reduced significantly the rate of H2O2 produced by mitochondrial respiration, the best antioxidant effect being obtained at the highest propolis concentration (250 μg/ml). Algerian propolis is a non-temperature sensitive scavenger that protects heart from oxidative damage induced by prolonged hypothermic ischemia.

Authors+Show Affiliations

Department of Biology, Faculty of Natural and Life Sciences, University of 8 mai 1945, Guelma, Algeria; Laboratory of Molecular Toxicology, Faculty of Sciences, University of Seddik Ben Yahia, Jijel, Algeria.Laboratory of Molecular Toxicology, Faculty of Sciences, University of Seddik Ben Yahia, Jijel, Algeria. Electronic address: lahouelmesbah@yahoo.fr.Department of Biology, Faculty of Natural and Life Sciences, University of 8 mai 1945, Guelma, Algeria.Laboratory of Cell Toxicology, Department of Biology, University of Badji Mokhtar, Annaba, Algeria.U955-IMRB, équipe 03, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30981678

Citation

Braik, Asma, et al. "Myocardial Protection By Propolis During Prolonged Hypothermic Preservation." Cryobiology, vol. 88, 2019, pp. 29-37.
Braik A, Lahouel M, Merabet R, et al. Myocardial protection by propolis during prolonged hypothermic preservation. Cryobiology. 2019;88:29-37.
Braik, A., Lahouel, M., Merabet, R., Djebar, M. R., & Morin, D. (2019). Myocardial protection by propolis during prolonged hypothermic preservation. Cryobiology, 88, 29-37. https://doi.org/10.1016/j.cryobiol.2019.04.003
Braik A, et al. Myocardial Protection By Propolis During Prolonged Hypothermic Preservation. Cryobiology. 2019;88:29-37. PubMed PMID: 30981678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myocardial protection by propolis during prolonged hypothermic preservation. AU - Braik,Asma, AU - Lahouel,Mesbah, AU - Merabet,Rym, AU - Djebar,Mohamed Réda, AU - Morin,Didier, Y1 - 2019/04/11/ PY - 2019/01/10/received PY - 2019/04/04/revised PY - 2019/04/08/accepted PY - 2019/4/15/pubmed PY - 2020/2/26/medline PY - 2019/4/15/entrez KW - Heart KW - Hypothermic ischemia KW - Mitochondria KW - Oxidative stress KW - Prolonged cold preservation KW - Propolis SP - 29 EP - 37 JF - Cryobiology JO - Cryobiology VL - 88 N2 - Oxidative stress is involved in the pathogenesis of ischemia-reperfusion during myocardial transplantation. Therefore, graft preservation solutions may be improved by supplementation with antioxidants to minimize graft dysfunction caused by cold ischemic injury. Propolis is a polyphenol-rich substance which has an important antioxidant activity. The protective effect of propolis against oxidative stress induced by prolonged cold preservation of heart was investigated. Mice were subjected to a hypothermic model of ischemia in which hearts were preserved for 24 h at 4 °C in Krebs-Hensleit (KH) solution in the absence or presence of propolis concentrations (50, 150 and 250 μg/ml). Levels of released Lactate dehydrogenase (LDH), Creatine phosphokinase (CPK) and Troponine-I (Trop I) were assessed in the preservation solution and histological assessement of heart ischemia injuries was performed. Oxidative stress biomarkers malondialdehyde (MDA) and advanced oxidation protein products (AOPP) and antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) were assessed in cardiac tissue. Mitochondria were isolated from stored hearts and production of reactive oxygen species (ROS) was tested. Propolis supplementation protected efficiently hearts during preservation by reducing significantly levels of lipids and proteins oxidation and restoring activities of antioxidant enzymes. Also, propolis preserved tissue integrity altered by hypothermic ischemia in a concentration-dependent manner. Propolis reduced significantly the rate of H2O2 produced by mitochondrial respiration, the best antioxidant effect being obtained at the highest propolis concentration (250 μg/ml). Algerian propolis is a non-temperature sensitive scavenger that protects heart from oxidative damage induced by prolonged hypothermic ischemia. SN - 1090-2392 UR - https://www.unboundmedicine.com/medline/citation/30981678/Myocardial_protection_by_propolis_during_prolonged_hypothermic_preservation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0011-2240(19)30009-4 DB - PRIME DP - Unbound Medicine ER -