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Physicochemical, scavenging and anti-proliferative analyses of polysaccharides extracted from psyllium (Plantago ovata Forssk) husk and seeds.
Int J Biol Macromol. 2019 Jul 15; 133:190-201.IJ

Abstract

Polysaccharides extracted from seeds and husk of psyllium were characterized for different physicochemical characteristics, and bioactivities. Extracted polysaccharides are comprised of d-xylose, l-arabinose, d-glucose, d-galactose, and l-rhamnose. Crude husk-polysaccharide was crystalline, whereas rest was amorphous in nature. Husk-polysaccharide was structurally stable, and purified fractions were thermostable. Crude polysaccharides were irregular in shape with non-porous smooth-surface, however purified husk-polysaccharides showed some porosity, and fibrous nature. Husk-polysaccharide showed higher viscosity compared to seed-polysaccharide, but viscosity decreased with the purification. Crude polysaccharides contained hydrogel-like behavior compared to corresponding purified fractions. The purified fractions of seed-polysaccharide showed the utmost antioxidant and scavenging activities with a half-maximal effective concentration of 347.40 ± 1.79 and 362.72 ± 2.75 μg, respectively. Crude seed-polysaccharide showed about 34% anti-proliferation on Huh-7, whereas its purified fractions showed 42% anti-proliferation on HeLa cell line. The study confirms that psyllium polysaccharides are potential natural antioxidant and anti-carcinogenic agent; however a detailed study is needed to explore psyllium for nutraceutical applications.

Authors+Show Affiliations

Biotechnology and Phycology Division, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat, India.Biotechnology and Phycology Division, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat, India.Analytical Chemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, India.Biotechnology and Phycology Division, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat, India. Electronic address: avinash@csmcri.res.in.Biotechnology and Phycology Division, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat, India. Electronic address: jha.bhavanath@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30981777

Citation

Patel, Manish Kumar, et al. "Physicochemical, Scavenging and Anti-proliferative Analyses of Polysaccharides Extracted From Psyllium (Plantago Ovata Forssk) Husk and Seeds." International Journal of Biological Macromolecules, vol. 133, 2019, pp. 190-201.
Patel MK, Tanna B, Gupta H, et al. Physicochemical, scavenging and anti-proliferative analyses of polysaccharides extracted from psyllium (Plantago ovata Forssk) husk and seeds. Int J Biol Macromol. 2019;133:190-201.
Patel, M. K., Tanna, B., Gupta, H., Mishra, A., & Jha, B. (2019). Physicochemical, scavenging and anti-proliferative analyses of polysaccharides extracted from psyllium (Plantago ovata Forssk) husk and seeds. International Journal of Biological Macromolecules, 133, 190-201. https://doi.org/10.1016/j.ijbiomac.2019.04.062
Patel MK, et al. Physicochemical, Scavenging and Anti-proliferative Analyses of Polysaccharides Extracted From Psyllium (Plantago Ovata Forssk) Husk and Seeds. Int J Biol Macromol. 2019 Jul 15;133:190-201. PubMed PMID: 30981777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physicochemical, scavenging and anti-proliferative analyses of polysaccharides extracted from psyllium (Plantago ovata Forssk) husk and seeds. AU - Patel,Manish Kumar, AU - Tanna,Bhakti, AU - Gupta,Hariom, AU - Mishra,Avinash, AU - Jha,Bhavanath, Y1 - 2019/04/11/ PY - 2019/03/11/received PY - 2019/04/05/revised PY - 2019/04/10/accepted PY - 2019/4/15/pubmed PY - 2019/12/21/medline PY - 2019/4/15/entrez KW - Antioxidant KW - Antiproliferative KW - Bioactivity KW - Isabgol KW - Polysaccharide SP - 190 EP - 201 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 133 N2 - Polysaccharides extracted from seeds and husk of psyllium were characterized for different physicochemical characteristics, and bioactivities. Extracted polysaccharides are comprised of d-xylose, l-arabinose, d-glucose, d-galactose, and l-rhamnose. Crude husk-polysaccharide was crystalline, whereas rest was amorphous in nature. Husk-polysaccharide was structurally stable, and purified fractions were thermostable. Crude polysaccharides were irregular in shape with non-porous smooth-surface, however purified husk-polysaccharides showed some porosity, and fibrous nature. Husk-polysaccharide showed higher viscosity compared to seed-polysaccharide, but viscosity decreased with the purification. Crude polysaccharides contained hydrogel-like behavior compared to corresponding purified fractions. The purified fractions of seed-polysaccharide showed the utmost antioxidant and scavenging activities with a half-maximal effective concentration of 347.40 ± 1.79 and 362.72 ± 2.75 μg, respectively. Crude seed-polysaccharide showed about 34% anti-proliferation on Huh-7, whereas its purified fractions showed 42% anti-proliferation on HeLa cell line. The study confirms that psyllium polysaccharides are potential natural antioxidant and anti-carcinogenic agent; however a detailed study is needed to explore psyllium for nutraceutical applications. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/30981777/Physicochemical_scavenging_and_anti_proliferative_analyses_of_polysaccharides_extracted_from_psyllium__Plantago_ovata_Forssk__husk_and_seeds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(19)31832-X DB - PRIME DP - Unbound Medicine ER -