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Analysis of phenolic acids and flavonoids in leaves of Lycium barbarum from different habitats by ultra-high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry.
Biomed Chromatogr. 2019 Aug; 33(8):e4552.BC

Abstract

The leaves of Lycium barbarum (LLB) have been utilized as crude drugs and functional tea for human health in China and Southeast Asia for thousands of years. To control its quality, a rapid and sensitive ultra-high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry method was established and validated for the first time for simultaneous determination of 10 phenolic acids and flavonoids (including neochlorogenic acid, protocatechuic aldehyde, p-hydroxybenzoic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, rutin and kaempferol-3-O-rutinoside) in LLB. The separation was performed on an Acquity UPLC C18 chromatographic column (100 × 2.1 mm internal diameter, 1.7 μm particle size) with 0.1% formic acid in water (A)-acetonitrile (B) as the mobile phase under gradient elution. Multiple reaction monitoring mode was adopted to simultaneously monitor the target components. The developed method was fully validated in terms of linearity (r2 ≥ 0.9860), precision (RSD ≤ 6.58%), repeatability (RSD ≤ 6.60%), stability (RSD ≤ 6.17%), recovery (95.56-108.06%, RSD ≤ 4.64%) and limit of detection (0.021-0.664 ng/mL) and limit of quantitation (0.069-2.210 ng/mL), and then successfully applied to evaluate the quality of 64 batches of LLB collected from 41 producing areas in four different provinces of China. The results showed that the LLB, especially collected from Inner Mongolia regions, were rich in the phenolic acids and flavonoids. Rutin, kaempferol-3-O-rutinoside and chlorogenic acid are the predominant compounds contained in LLB. The above findings will provide helpful information for the effective utilization of LLB.

Authors+Show Affiliations

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing, China.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing, China.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing, China.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing, China.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing, China.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing, China.School of Pharmacy, Ningxia Medical University, Yinchuan, China.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30985939

Citation

Zhao, Xue-Qin, et al. "Analysis of Phenolic Acids and Flavonoids in Leaves of Lycium Barbarum From Different Habitats By Ultra-high-performance Liquid Chromatography Coupled With Triple Quadrupole Tandem Mass Spectrometry." Biomedical Chromatography : BMC, vol. 33, no. 8, 2019, pp. e4552.
Zhao XQ, Guo S, Yan H, et al. Analysis of phenolic acids and flavonoids in leaves of Lycium barbarum from different habitats by ultra-high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry. Biomed Chromatogr. 2019;33(8):e4552.
Zhao, X. Q., Guo, S., Yan, H., Lu, Y. Y., Zhang, F., Qian, D. W., Wang, H. Q., & Duan, J. A. (2019). Analysis of phenolic acids and flavonoids in leaves of Lycium barbarum from different habitats by ultra-high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry. Biomedical Chromatography : BMC, 33(8), e4552. https://doi.org/10.1002/bmc.4552
Zhao XQ, et al. Analysis of Phenolic Acids and Flavonoids in Leaves of Lycium Barbarum From Different Habitats By Ultra-high-performance Liquid Chromatography Coupled With Triple Quadrupole Tandem Mass Spectrometry. Biomed Chromatogr. 2019;33(8):e4552. PubMed PMID: 30985939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of phenolic acids and flavonoids in leaves of Lycium barbarum from different habitats by ultra-high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry. AU - Zhao,Xue-Qin, AU - Guo,Sheng, AU - Yan,Hui, AU - Lu,You-Yuan, AU - Zhang,Fang, AU - Qian,Da-Wei, AU - Wang,Han-Qing, AU - Duan,Jin-Ao, Y1 - 2019/06/03/ PY - 2019/02/19/received PY - 2019/03/25/revised PY - 2019/04/05/accepted PY - 2019/4/16/pubmed PY - 2019/8/6/medline PY - 2019/4/16/entrez KW - UHPLC-MS/MS KW - flavonoids KW - leaves of Lycium barbarum KW - phenolic acids KW - regional differences SP - e4552 EP - e4552 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 33 IS - 8 N2 - The leaves of Lycium barbarum (LLB) have been utilized as crude drugs and functional tea for human health in China and Southeast Asia for thousands of years. To control its quality, a rapid and sensitive ultra-high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry method was established and validated for the first time for simultaneous determination of 10 phenolic acids and flavonoids (including neochlorogenic acid, protocatechuic aldehyde, p-hydroxybenzoic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, rutin and kaempferol-3-O-rutinoside) in LLB. The separation was performed on an Acquity UPLC C18 chromatographic column (100 × 2.1 mm internal diameter, 1.7 μm particle size) with 0.1% formic acid in water (A)-acetonitrile (B) as the mobile phase under gradient elution. Multiple reaction monitoring mode was adopted to simultaneously monitor the target components. The developed method was fully validated in terms of linearity (r2 ≥ 0.9860), precision (RSD ≤ 6.58%), repeatability (RSD ≤ 6.60%), stability (RSD ≤ 6.17%), recovery (95.56-108.06%, RSD ≤ 4.64%) and limit of detection (0.021-0.664 ng/mL) and limit of quantitation (0.069-2.210 ng/mL), and then successfully applied to evaluate the quality of 64 batches of LLB collected from 41 producing areas in four different provinces of China. The results showed that the LLB, especially collected from Inner Mongolia regions, were rich in the phenolic acids and flavonoids. Rutin, kaempferol-3-O-rutinoside and chlorogenic acid are the predominant compounds contained in LLB. The above findings will provide helpful information for the effective utilization of LLB. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/30985939/Analysis_of_phenolic_acids_and_flavonoids_in_leaves_of_Lycium_barbarum_from_different_habitats_by_ultra_high_performance_liquid_chromatography_coupled_with_triple_quadrupole_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/bmc.4552 DB - PRIME DP - Unbound Medicine ER -