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Implications of prostaglandin D2 and leukotrienes in exhaled breath condensates of asthma.
Ann Allergy Asthma Immunol. 2019 07; 123(1):81-88.e1.AA

Abstract

BACKGROUND

Various inflammatory eicosanoid levels in biomaterials from airways of asthma and their associations with clinical parameters remain uncertain. We hypothesized that prostaglandin and leukotriene levels differ between in exhaled breath condensates (EBCs) and in sputum in mild, moderate, and severe levels of asthma and that EBC and sputum eicosanoid levels are associated with indexes of pulmonary function and inflammation.

OBJECTIVE

To determine the differences between EBC and sputum eicosanoid levels in healthy participants and patients with asthma with different asthma severity levels.

METHODS

Collected EBC and sputum, as well as pulmonary function, were examined in adult patients with asthma and healthy participants. Exhaled breath condensate prostaglandin D2-methoxime (PGD2-MOX), cysteinyl leukotrienes (CysLTs), leukotriene B4 (LTB4), and thromboxane B2 levels, and some sputum eicosanoid and tryptase levels were measured. Differences in eicosanoid levels among participants and their associations with pulmonary function and tryptase and granulocyte levels in sputum were then evaluated.

RESULTS

Analysis of 94 EBCs and 43 sputa revealed that EBC and sputum PGD2-MOX and CysLT levels were significantly higher in patients with asthma than in healthy participants. Exhaled breath condensate PGD2-MOX, CysLT, and LTB4 levels were significantly higher in patients with severe asthma. Exhaled breath condensate PGD2-MOX level was also significantly correlated with sputum tryptase levels and lower pulmonary function in patients with asthma. Sputum PGD2-MOX and CysLT levels were significantly correlated with the proportion of eosinophils among all cells in sputum in patients with asthma.

CONCLUSION

The results suggest that EBC PGD2 levels are associated with impairment of pulmonary function in adults with asthma who have undergone guideline treatment. Exhaled breath condensate or sputum PGD2 and CysLTs may represent severity or airway inflammation in asthma.

Authors+Show Affiliations

Department of Respiratory Medicine, Saitama Medical University, Iruma-gun, Saitama, Japan; Allergy Center, Saitama Medical University Hospital, Iruma-gun, Saitama, Japan.Department of Respiratory Medicine, Saitama Medical University, Iruma-gun, Saitama, Japan; Allergy Center, Saitama Medical University Hospital, Iruma-gun, Saitama, Japan. Electronic address: tsoma@saitama-med.ac.jp.Department of Respiratory Medicine, Saitama Medical University, Iruma-gun, Saitama, Japan; Allergy Center, Saitama Medical University Hospital, Iruma-gun, Saitama, Japan.Department of Respiratory Medicine, Saitama Medical University, Iruma-gun, Saitama, Japan; Department of General Internal Medicine, Saitama Medical University, Iruma-gun, Saitama, Japan.Department of Respiratory Medicine, Saitama Medical University, Iruma-gun, Saitama, Japan; Allergy Center, Saitama Medical University Hospital, Iruma-gun, Saitama, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30986547

Citation

Uchida, Yoshitaka, et al. "Implications of Prostaglandin D2 and Leukotrienes in Exhaled Breath Condensates of Asthma." Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, vol. 123, no. 1, 2019, pp. 81-88.e1.
Uchida Y, Soma T, Nakagome K, et al. Implications of prostaglandin D2 and leukotrienes in exhaled breath condensates of asthma. Ann Allergy Asthma Immunol. 2019;123(1):81-88.e1.
Uchida, Y., Soma, T., Nakagome, K., Kobayashi, T., & Nagata, M. (2019). Implications of prostaglandin D2 and leukotrienes in exhaled breath condensates of asthma. Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, 123(1), 81-e1. https://doi.org/10.1016/j.anai.2019.04.008
Uchida Y, et al. Implications of Prostaglandin D2 and Leukotrienes in Exhaled Breath Condensates of Asthma. Ann Allergy Asthma Immunol. 2019;123(1):81-88.e1. PubMed PMID: 30986547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Implications of prostaglandin D2 and leukotrienes in exhaled breath condensates of asthma. AU - Uchida,Yoshitaka, AU - Soma,Tomoyuki, AU - Nakagome,Kazuyuki, AU - Kobayashi,Takehito, AU - Nagata,Makoto, Y1 - 2019/04/12/ PY - 2019/01/04/received PY - 2019/04/05/revised PY - 2019/04/07/accepted PY - 2019/4/16/pubmed PY - 2019/12/28/medline PY - 2019/4/16/entrez SP - 81 EP - 88.e1 JF - Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology JO - Ann Allergy Asthma Immunol VL - 123 IS - 1 N2 - BACKGROUND: Various inflammatory eicosanoid levels in biomaterials from airways of asthma and their associations with clinical parameters remain uncertain. We hypothesized that prostaglandin and leukotriene levels differ between in exhaled breath condensates (EBCs) and in sputum in mild, moderate, and severe levels of asthma and that EBC and sputum eicosanoid levels are associated with indexes of pulmonary function and inflammation. OBJECTIVE: To determine the differences between EBC and sputum eicosanoid levels in healthy participants and patients with asthma with different asthma severity levels. METHODS: Collected EBC and sputum, as well as pulmonary function, were examined in adult patients with asthma and healthy participants. Exhaled breath condensate prostaglandin D2-methoxime (PGD2-MOX), cysteinyl leukotrienes (CysLTs), leukotriene B4 (LTB4), and thromboxane B2 levels, and some sputum eicosanoid and tryptase levels were measured. Differences in eicosanoid levels among participants and their associations with pulmonary function and tryptase and granulocyte levels in sputum were then evaluated. RESULTS: Analysis of 94 EBCs and 43 sputa revealed that EBC and sputum PGD2-MOX and CysLT levels were significantly higher in patients with asthma than in healthy participants. Exhaled breath condensate PGD2-MOX, CysLT, and LTB4 levels were significantly higher in patients with severe asthma. Exhaled breath condensate PGD2-MOX level was also significantly correlated with sputum tryptase levels and lower pulmonary function in patients with asthma. Sputum PGD2-MOX and CysLT levels were significantly correlated with the proportion of eosinophils among all cells in sputum in patients with asthma. CONCLUSION: The results suggest that EBC PGD2 levels are associated with impairment of pulmonary function in adults with asthma who have undergone guideline treatment. Exhaled breath condensate or sputum PGD2 and CysLTs may represent severity or airway inflammation in asthma. SN - 1534-4436 UR - https://www.unboundmedicine.com/medline/citation/30986547/Implications_of_prostaglandin_D2_and_leukotrienes_in_exhaled_breath_condensates_of_asthma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1081-1206(19)30292-3 DB - PRIME DP - Unbound Medicine ER -