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The regulation of N-acyl-homoserine lactones (AHLs)-based quorum sensing on EPS secretion via ATP synthetic for the stability of aerobic granular sludge.

Abstract

According to the relationship among microbial activity, quorum sensing (QS) and structural stability of aerobic granular sludge, the mechanism of QS regulation for microbial activity and granular stability was investigated in AGS process. Results showed that ATP content decreased sharply from 1.8 μmol/gVSS of stable granules to 0.8 μmol/gVSS of disintegrating granules, and the relative abundance of QS-activity microbes, Rhodobacter spp. and Xanthomonadaceae decreased in initially unstable granules compared with stable granules. The main AHLs were detected in this study, and C8-HSL, 3OHC8-HSL and 3OHC12-HSL decreased significantly when structure of granules changed from stability to disintegration. Accompanying with the decrease of AHLs level, the extracellular polymeric substances (EPS) content in initially unstable granules decreased sharply from 226.8 to 163.6 mg/gVSS with the ratio of extracellular protein to exopolysaccharide (PN/PS) decreasing from 3.6 to 2.2, despite EPS-secretion microbes enriched. The effect of QS on microbial activity was proved by AHL add-back study, results indicated that ATP and EPS content in sludge increased significantly (p < 0.05) with AHLs added, but EPS production was limited when ATP synthesis was disrupted. It was concluded that the AHLs-based QS favored the granular stability via the enhancement of ATP synthesis in microbes. This study provides a new perspective for QS regulation in aerobic granular sludge system, because the ATP regulated by QS could be the energy currency for cellular metabolism, such as nutrient removal, degradation of emerging pollutants, microbial growth and other aspects.

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  • Authors+Show Affiliations

    ,

    Institute of Environmental Pollution Control and Treatment, Zhejiang University, Hangzhou 310058, China.

    ,

    Institute of Environmental Pollution Control and Treatment, Zhejiang University, Hangzhou 310058, China.

    ,

    Hangzhou Urban & Rural Construction Design Institute Co., LTD, Hangzhou 310058, China.

    ,

    Environmental Protection Bureau of Changxing County, Huzhou 313100, China.

    ,

    Institute of Environmental Pollution Control and Treatment, Zhejiang University, Hangzhou 310058, China.

    ,

    Institute of Environmental Pollution Control and Treatment, Zhejiang University, Hangzhou 310058, China; Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou 310058, China; Zhejiang Provincial Engineering Laboratory of Water Pollution Control, 388 Yuhangtang Road, Hangzhou 310058, China.

    Institute of Environmental Pollution Control and Treatment, Zhejiang University, Hangzhou 310058, China; Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou 310058, China; Zhejiang Provincial Engineering Laboratory of Water Pollution Control, 388 Yuhangtang Road, Hangzhou 310058, China. Electronic address: felix79cn@zju.edu.cn.

    Source

    The Science of the total environment 673: 2019 Jul 10 pg 83-91

    MeSH

    Acyl-Butyrolactones
    Adenosine Triphosphate
    Aerobiosis
    Bioreactors
    Extracellular Polymeric Substance Matrix
    Quorum Sensing
    Sewage
    Waste Disposal, Fluid

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30986684

    Citation

    Zhang, Zhiming, et al. "The Regulation of N-acyl-homoserine Lactones (AHLs)-based Quorum Sensing On EPS Secretion Via ATP Synthetic for the Stability of Aerobic Granular Sludge." The Science of the Total Environment, vol. 673, 2019, pp. 83-91.
    Zhang Z, Cao R, Jin L, et al. The regulation of N-acyl-homoserine lactones (AHLs)-based quorum sensing on EPS secretion via ATP synthetic for the stability of aerobic granular sludge. Sci Total Environ. 2019;673:83-91.
    Zhang, Z., Cao, R., Jin, L., Zhu, W., Ji, Y., Xu, X., & Zhu, L. (2019). The regulation of N-acyl-homoserine lactones (AHLs)-based quorum sensing on EPS secretion via ATP synthetic for the stability of aerobic granular sludge. The Science of the Total Environment, 673, pp. 83-91. doi:10.1016/j.scitotenv.2019.04.052.
    Zhang Z, et al. The Regulation of N-acyl-homoserine Lactones (AHLs)-based Quorum Sensing On EPS Secretion Via ATP Synthetic for the Stability of Aerobic Granular Sludge. Sci Total Environ. 2019 Jul 10;673:83-91. PubMed PMID: 30986684.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The regulation of N-acyl-homoserine lactones (AHLs)-based quorum sensing on EPS secretion via ATP synthetic for the stability of aerobic granular sludge. AU - Zhang,Zhiming, AU - Cao,Runjuan, AU - Jin,Luonan, AU - Zhu,Weitang, AU - Ji,Yatong, AU - Xu,Xiangyang, AU - Zhu,Liang, Y1 - 2019/04/05/ PY - 2019/01/14/received PY - 2019/03/13/revised PY - 2019/04/04/accepted PY - 2019/4/16/pubmed PY - 2019/5/18/medline PY - 2019/4/16/entrez KW - ATP KW - Aerobic granular sludge KW - Extracellular polymeric substances (EPS) KW - N-acyl-homoserine lactones (AHLs) KW - Quorum sensing SP - 83 EP - 91 JF - The Science of the total environment JO - Sci. Total Environ. VL - 673 N2 - According to the relationship among microbial activity, quorum sensing (QS) and structural stability of aerobic granular sludge, the mechanism of QS regulation for microbial activity and granular stability was investigated in AGS process. Results showed that ATP content decreased sharply from 1.8 μmol/gVSS of stable granules to 0.8 μmol/gVSS of disintegrating granules, and the relative abundance of QS-activity microbes, Rhodobacter spp. and Xanthomonadaceae decreased in initially unstable granules compared with stable granules. The main AHLs were detected in this study, and C8-HSL, 3OHC8-HSL and 3OHC12-HSL decreased significantly when structure of granules changed from stability to disintegration. Accompanying with the decrease of AHLs level, the extracellular polymeric substances (EPS) content in initially unstable granules decreased sharply from 226.8 to 163.6 mg/gVSS with the ratio of extracellular protein to exopolysaccharide (PN/PS) decreasing from 3.6 to 2.2, despite EPS-secretion microbes enriched. The effect of QS on microbial activity was proved by AHL add-back study, results indicated that ATP and EPS content in sludge increased significantly (p < 0.05) with AHLs added, but EPS production was limited when ATP synthesis was disrupted. It was concluded that the AHLs-based QS favored the granular stability via the enhancement of ATP synthesis in microbes. This study provides a new perspective for QS regulation in aerobic granular sludge system, because the ATP regulated by QS could be the energy currency for cellular metabolism, such as nutrient removal, degradation of emerging pollutants, microbial growth and other aspects. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/30986684/The_regulation_of_N-acyl-homoserine_lactones_(AHLs)-based_quorum_sensing_on_EPS_secretion_via_ATP_synthetic_for_the_stability_of_aerobic_granular_sludge L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(19)31571-2 DB - PRIME DP - Unbound Medicine ER -