Tags

Type your tag names separated by a space and hit enter

Using gas chromatography and mass spectrometry to determine 25-hydroxyvitamin D levels for clinical assessment of vitamin D deficiency.

Abstract

Vitamin D is responsible for multiple metabolic functions in humans. Rickets are the most common disease caused by vitamin D deficiency. It is caused by poor calcium intake resulting in poor serum-ionized calcium. The purpose of this study is to develop a rapid, sensitive, and feasible method to determine the 25-hydroxy-vitamin D3 (25(OH)D3) levels in blood samples for clinical assessment. In this study, gas chromatography coupled mass spectrometry with trimethylsilyl derivatization (TMS-GC-MS) is the most suitable protocol for quantitative analyses of 25(OH)D3. Performance of method was evaluated and compared with liquid chromatography and immunoassay. Method validation has been carried out with plasma specimens. The limit of quantitation of TMS-GC-MS method is 1.5 ppb with good linear correlation. Furthermore, the dietary intake and nutritional status of vegetarian and non-vegetarians in Taiwan were assessed by our validated method. As a result, this vitamin D nutrition survey demonstrates that most Taiwanese people have insufficient vitamin D. Due to dietary habits; the male vegans may have the highest risk of vitamin D deficiency.

Links

  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan.

    ,

    Department of Laboratory Medicine and Biotechnology, College of Medicine, Tzu-Chi University, Hualien, Taiwan.

    ,

    Department of Nutrition Therapy, Buddhist Tzu-Chi Medical Foundation, Hualien, Taiwan; Department of Medicine, College of Medicine, Tzu-Chi University, Hualien, Taiwan.

    ,

    Department of Laboratory Medicine and Biotechnology, College of Medicine, Tzu-Chi University, Hualien, Taiwan; Department of Laboratory Medicine, Buddhist Tzu-Chi General Hospital, Hualien, Taiwan.

    ,

    Department of Family Medicine, Dalin Tzu-Chi Hospital, Buddhist Tzu-Chi Medical Foundation, Chiayi County, Taiwan; Department of Family Medicine, College of Medicine, Tzu-Chi University, Hualien, Taiwan.

    ,

    Department of Biotechnology, Ming-Chuan University, Taoyuan City, Taiwan. Electronic address: loknath@mail.mcu.edu.tw.

    Department of Laboratory Medicine and Biotechnology, College of Medicine, Tzu-Chi University, Hualien, Taiwan. Electronic address: anren@gms.tcu.edu.tw.

    Source

    Journal of food and drug analysis 27:2 2019 Apr pg 494-501

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30987720

    Citation

    Yang, Ming-Yeh, et al. "Using Gas Chromatography and Mass Spectrometry to Determine 25-hydroxyvitamin D Levels for Clinical Assessment of Vitamin D Deficiency." Journal of Food and Drug Analysis, vol. 27, no. 2, 2019, pp. 494-501.
    Yang MY, Huang CY, Chiu THT, et al. Using gas chromatography and mass spectrometry to determine 25-hydroxyvitamin D levels for clinical assessment of vitamin D deficiency. J Food Drug Anal. 2019;27(2):494-501.
    Yang, M. Y., Huang, C. Y., Chiu, T. H. T., Chang, K. C., Lin, M. N., Chen, L. Y., & Hu, A. (2019). Using gas chromatography and mass spectrometry to determine 25-hydroxyvitamin D levels for clinical assessment of vitamin D deficiency. Journal of Food and Drug Analysis, 27(2), pp. 494-501. doi:10.1016/j.jfda.2018.12.010.
    Yang MY, et al. Using Gas Chromatography and Mass Spectrometry to Determine 25-hydroxyvitamin D Levels for Clinical Assessment of Vitamin D Deficiency. J Food Drug Anal. 2019;27(2):494-501. PubMed PMID: 30987720.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Using gas chromatography and mass spectrometry to determine 25-hydroxyvitamin D levels for clinical assessment of vitamin D deficiency. AU - Yang,Ming-Yeh, AU - Huang,Ching-Yuan, AU - Chiu,Tina H T, AU - Chang,Kai-Chih, AU - Lin,Ming-Nan, AU - Chen,Liang-Yü, AU - Hu,Anren, Y1 - 2019/01/08/ PY - 2018/06/21/received PY - 2018/12/23/revised PY - 2018/12/24/accepted PY - 2019/4/17/entrez KW - Clinical specimen KW - Derivatization KW - Osteoporosis KW - Vegetarian KW - Vitamin D metabolites SP - 494 EP - 501 JF - Journal of food and drug analysis JO - J Food Drug Anal VL - 27 IS - 2 N2 - Vitamin D is responsible for multiple metabolic functions in humans. Rickets are the most common disease caused by vitamin D deficiency. It is caused by poor calcium intake resulting in poor serum-ionized calcium. The purpose of this study is to develop a rapid, sensitive, and feasible method to determine the 25-hydroxy-vitamin D3 (25(OH)D3) levels in blood samples for clinical assessment. In this study, gas chromatography coupled mass spectrometry with trimethylsilyl derivatization (TMS-GC-MS) is the most suitable protocol for quantitative analyses of 25(OH)D3. Performance of method was evaluated and compared with liquid chromatography and immunoassay. Method validation has been carried out with plasma specimens. The limit of quantitation of TMS-GC-MS method is 1.5 ppb with good linear correlation. Furthermore, the dietary intake and nutritional status of vegetarian and non-vegetarians in Taiwan were assessed by our validated method. As a result, this vitamin D nutrition survey demonstrates that most Taiwanese people have insufficient vitamin D. Due to dietary habits; the male vegans may have the highest risk of vitamin D deficiency. SN - 1021-9498 UR - https://www.unboundmedicine.com/medline/citation/30987720/Using_gas_chromatography_and_mass_spectrometry_to_determine_25-hydroxyvitamin_D_levels_for_clinical_assessment_of_vitamin_D_deficiency L2 - https://linkinghub.elsevier.com/retrieve/pii/S1021-9498(18)30184-4 DB - PRIME DP - Unbound Medicine ER -