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In vitro evaluation of the protoscolicidal effect of Eucalyptus globulus essential oil on protoscolices of hydatid cyst compared with hypertonic saline, povidone iodine and silver nitrate.

Abstract

OBJECTIVE

There are various protoscolicidal agents for inactivation of protoscoleces of hydatid cysts before and during surgical operation. The present study was aimed to evaluate the protoscolicidal effect of two concentrations of Eucalyptus globulus on protoscoleces of Echinococcus granulosus sensu lato under in vitro condition and to compare its efficacy with hypertonic saline, povidone iodine and silver nitrate.

METHODS

Live protoscoleces obtained from the liver of naturally infected sheep were exposed to 0.5% and 1% of Eucalyptus globulus essential oil, 5% hypertonic saline, 10% povidone iodine and 0.5% silver nitrate for 1 and 3minutes. Phosphate buffered saline was used as a negative control. One percent eosin staining method was used to test the viability of protoscoleces in different groups.

RESULTS

While the mean percentage of dead protoscoleces was 6.08% in the control group, the scolicidal power of 5% hypertonic saline was only 6.54% and 6.60% after 1 and 3min respectively. 0.5% E. globulus EO demonstrated 97.38% and 100% scolicidal activity after 1 and 3min respectively. The mean protoscolicidal power of 1% E. globulus EO, 10% povidone iodine and 0.5% silver nitrate was 100% after one minute.

CONCLUSIONS

According to the results of this study, E. globulus EO demonstrated high scolicidal power in a short period of time. Hence, this herbal product could be considered as a potent natural scolicidal agent that could be used before and during surgery of hydatid disease.

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  • Authors+Show Affiliations

    ,

    Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

    ,

    Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

    ,

    Kufa University, College of medicine, Kufa, Iraq.

    ,

    Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

    Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: Khazraee@sums.ac.ir.

    Source

    Journal of visceral surgery : 2019 Apr 12 pg

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30987848

    Citation

    Moazeni, M, et al. "In Vitro Evaluation of the Protoscolicidal Effect of Eucalyptus Globulus Essential Oil On Protoscolices of Hydatid Cyst Compared With Hypertonic Saline, Povidone Iodine and Silver Nitrate." Journal of Visceral Surgery, 2019.
    Moazeni M, Hosseini SV, Al-Qanbar MH, et al. In vitro evaluation of the protoscolicidal effect of Eucalyptus globulus essential oil on protoscolices of hydatid cyst compared with hypertonic saline, povidone iodine and silver nitrate. J Visc Surg. 2019.
    Moazeni, M., Hosseini, S. V., Al-Qanbar, M. H., Alavi, A. M., & Khazraei, H. (2019). In vitro evaluation of the protoscolicidal effect of Eucalyptus globulus essential oil on protoscolices of hydatid cyst compared with hypertonic saline, povidone iodine and silver nitrate. Journal of Visceral Surgery, doi:10.1016/j.jviscsurg.2019.01.002.
    Moazeni M, et al. In Vitro Evaluation of the Protoscolicidal Effect of Eucalyptus Globulus Essential Oil On Protoscolices of Hydatid Cyst Compared With Hypertonic Saline, Povidone Iodine and Silver Nitrate. J Visc Surg. 2019 Apr 12; PubMed PMID: 30987848.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - In vitro evaluation of the protoscolicidal effect of Eucalyptus globulus essential oil on protoscolices of hydatid cyst compared with hypertonic saline, povidone iodine and silver nitrate. AU - Moazeni,M, AU - Hosseini,S V, AU - Al-Qanbar,M H, AU - Alavi,A M, AU - Khazraei,H, Y1 - 2019/04/12/ PY - 2019/4/17/entrez KW - Echinococcus granulosus KW - Eucalyptus globulus KW - Hypertonic Saline KW - Povidone iodine KW - Silver nitrate JF - Journal of visceral surgery JO - J Visc Surg N2 - OBJECTIVE: There are various protoscolicidal agents for inactivation of protoscoleces of hydatid cysts before and during surgical operation. The present study was aimed to evaluate the protoscolicidal effect of two concentrations of Eucalyptus globulus on protoscoleces of Echinococcus granulosus sensu lato under in vitro condition and to compare its efficacy with hypertonic saline, povidone iodine and silver nitrate. METHODS: Live protoscoleces obtained from the liver of naturally infected sheep were exposed to 0.5% and 1% of Eucalyptus globulus essential oil, 5% hypertonic saline, 10% povidone iodine and 0.5% silver nitrate for 1 and 3minutes. Phosphate buffered saline was used as a negative control. One percent eosin staining method was used to test the viability of protoscoleces in different groups. RESULTS: While the mean percentage of dead protoscoleces was 6.08% in the control group, the scolicidal power of 5% hypertonic saline was only 6.54% and 6.60% after 1 and 3min respectively. 0.5% E. globulus EO demonstrated 97.38% and 100% scolicidal activity after 1 and 3min respectively. The mean protoscolicidal power of 1% E. globulus EO, 10% povidone iodine and 0.5% silver nitrate was 100% after one minute. CONCLUSIONS: According to the results of this study, E. globulus EO demonstrated high scolicidal power in a short period of time. Hence, this herbal product could be considered as a potent natural scolicidal agent that could be used before and during surgery of hydatid disease. SN - 1878-7886 UR - https://www.unboundmedicine.com/medline/citation/30987848/In_vitro_evaluation_of_the_protoscolicidal_effect_of_Eucalyptus_globulus_essential_oil_on_protoscolices_of_hydatid_cyst_compared_with_hypertonic_saline_povidone_iodine_and_silver_nitrate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1878-7886(19)30002-5 DB - PRIME DP - Unbound Medicine ER -