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Functional analysis of citrus AP2 transcription factors identified CsAP2-09 involved in citrus canker disease response and tolerance.
Gene. 2019 Jul 30; 707:178-188.GENE

Abstract

Genetic engineering approaches offer an alternative method to the citrus canker resistance breeding. The ethylene response factor (ERF) family is a member of families of transcription factors that are particular to plants and contribute significantly to biotic stress response and to plant growth. CsAP2-09 belongs to the citrus AP2/ERF transcription factor family. Initially, we proved the induction of CsAP2-09 in wild-types by Xcc and some hormones involved in pathogen response. We successfully cloned the CsAP2-09 and proved that CsAP2-09 protein is targeted to the nucleus. The CsAP2-09 was functionally characterized with over-expression and RNAi silencing strategy. In the overexpression lines, the diseased lesions and disease index were significantly decreased while in RNAi lines of CsAP2-09 the diseased lesions and disease index were significantly enhanced. Thus, the over-expression conferred Xcc resistance to transgenic citrus while silencing of CsAP2-09 in sweet orange leads to Xcc susceptibility. When the transcriptomes of WT and overexpression transcriptomes were compared, they revealed that some genes involved in phenylpropanoid biosynthesis, pathogen responses, transcript regulation etc. were modified. Our results provide a possibility for improving citrus canker disease resistance by over-expression of CsAP2s. Furthermore, various functions of CsAP2-09 provide significant information about the role of AP2/ERFs in plant disease resistance and stress tolerance.

Authors+Show Affiliations

Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331 Chongqing, PR China.Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331 Chongqing, PR China.Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, PR China.Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, PR China.Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, PR China.Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, PR China.Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, PR China.Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, PR China.Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, PR China.Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331 Chongqing, PR China. Electronic address: zhengguoli@cqu.edu.cn.Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, PR China. Electronic address: liqiang@cric.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30991097

Citation

He, Yongrui, et al. "Functional Analysis of Citrus AP2 Transcription Factors Identified CsAP2-09 Involved in Citrus Canker Disease Response and Tolerance." Gene, vol. 707, 2019, pp. 178-188.
He Y, Jia R, Qi J, et al. Functional analysis of citrus AP2 transcription factors identified CsAP2-09 involved in citrus canker disease response and tolerance. Gene. 2019;707:178-188.
He, Y., Jia, R., Qi, J., Chen, S., Lei, T., Xu, L., Peng, A., Yao, L., Long, Q., Li, Z., & Li, Q. (2019). Functional analysis of citrus AP2 transcription factors identified CsAP2-09 involved in citrus canker disease response and tolerance. Gene, 707, 178-188. https://doi.org/10.1016/j.gene.2019.04.021
He Y, et al. Functional Analysis of Citrus AP2 Transcription Factors Identified CsAP2-09 Involved in Citrus Canker Disease Response and Tolerance. Gene. 2019 Jul 30;707:178-188. PubMed PMID: 30991097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional analysis of citrus AP2 transcription factors identified CsAP2-09 involved in citrus canker disease response and tolerance. AU - He,Yongrui, AU - Jia,Ruirui, AU - Qi,Jingjing, AU - Chen,Shanchun, AU - Lei,Tiangang, AU - Xu,Lanzhen, AU - Peng,Aihong, AU - Yao,Lixiao, AU - Long,Qin, AU - Li,Zhengguo, AU - Li,Qiang, Y1 - 2019/04/13/ PY - 2018/11/15/received PY - 2019/03/12/revised PY - 2019/04/05/accepted PY - 2019/4/17/pubmed PY - 2019/6/25/medline PY - 2019/4/17/entrez KW - AP2 KW - Citrus bacterial canker KW - Genetic transformation KW - RNA-Seq KW - Xcc resistance SP - 178 EP - 188 JF - Gene JO - Gene VL - 707 N2 - Genetic engineering approaches offer an alternative method to the citrus canker resistance breeding. The ethylene response factor (ERF) family is a member of families of transcription factors that are particular to plants and contribute significantly to biotic stress response and to plant growth. CsAP2-09 belongs to the citrus AP2/ERF transcription factor family. Initially, we proved the induction of CsAP2-09 in wild-types by Xcc and some hormones involved in pathogen response. We successfully cloned the CsAP2-09 and proved that CsAP2-09 protein is targeted to the nucleus. The CsAP2-09 was functionally characterized with over-expression and RNAi silencing strategy. In the overexpression lines, the diseased lesions and disease index were significantly decreased while in RNAi lines of CsAP2-09 the diseased lesions and disease index were significantly enhanced. Thus, the over-expression conferred Xcc resistance to transgenic citrus while silencing of CsAP2-09 in sweet orange leads to Xcc susceptibility. When the transcriptomes of WT and overexpression transcriptomes were compared, they revealed that some genes involved in phenylpropanoid biosynthesis, pathogen responses, transcript regulation etc. were modified. Our results provide a possibility for improving citrus canker disease resistance by over-expression of CsAP2s. Furthermore, various functions of CsAP2-09 provide significant information about the role of AP2/ERFs in plant disease resistance and stress tolerance. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/30991097/Functional_analysis_of_citrus_AP2_transcription_factors_identified_CsAP2_09_involved_in_citrus_canker_disease_response_and_tolerance_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(19)30367-1 DB - PRIME DP - Unbound Medicine ER -