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[The effect of NK-1R-siRNA on expression of inflammatory factors in allergic rhinitis].

Abstract

Objective:

To explore the effect of neurokinin-1 receptor small interfering RNA (NK-1R-siRNA) on the expression of inflammation factors in allergic rhinitis (AR).

Methods:

Twenty-four male SD rats were divided into three groups randomly (by random number table methord): NK-1R-siRNA group, negative control siRNA (NC-siRNA) group and saline group, with 8 rats in each group. SD rats were sensitized and challenged with ovalbumin (OVA) to induce AR. The rats were treated intranasally with NK-1R-siRNA, NC-siRNA or saline before and during the challenge period. The AR symptoms were observed. The levels of OVA-specific IgE were measured by enzyme-linked immunosorbent assay (ELISA). The levels of NK-1R expression in the nasal mucosal tissues were determined by real time PCR (RT-PCR) and immunohistochemistry. Antibody array was used in studying the expression of inflammation cell factors in nasal mucosa. SPSS 11.0 software was used for one-factor analysis of variance.

Results:

Compared with saline group, AR symptoms relived significantly in NK-1R-siRNA group (nose rubbing (31.4±8.9)/15 min vs (69.5±17.9)/15 min, sneezing (7.2±1.9)/15 min vs (23.7±9.2)/15 min, nasal secretions (7.1±2.3) mg vs (24.1±4.4) mg, t value was 38.100, 17.125, 16.837, respectively, all P<0.01), and the level of serum OVA-specific IgE was also reduced ((8.56±0.73) ng/ml vs (18.05±1.22) ng/ml, t=9.787, P<0.01). The RT-PCR and immunohistochemistry results showed that the expression of NK-1R in nasal mucosa of NK-1R-siRNA group was remarkably reduced than that of the NC-siRNA group and saline group. After the treatment of NK-1R-siRNA, the expression of interleukin (IL) 1α, IL-1β, IL-4, IL-6 and IL-13 decreased, while the interferon-γ (IFN-γ) and IL-10 increased.

Conclusion:

NK-1R-siRNA could regulate the release of inflammation factors in AR nasal mucosa, thus relive the allergic inflammation.

Authors+Show Affiliations

Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

30991778

Citation

Wang, H, et al. "[The Effect of NK-1R-siRNA On Expression of Inflammatory Factors in Allergic Rhinitis]." Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Chinese Journal of Otorhinolaryngology Head and Neck Surgery, vol. 54, no. 4, 2019, pp. 280-285.
Wang H, Wu J, Zhang RX. [The effect of NK-1R-siRNA on expression of inflammatory factors in allergic rhinitis]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2019;54(4):280-285.
Wang, H., Wu, J., & Zhang, R. X. (2019). [The effect of NK-1R-siRNA on expression of inflammatory factors in allergic rhinitis]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Chinese Journal of Otorhinolaryngology Head and Neck Surgery, 54(4), pp. 280-285. doi:10.3760/cma.j.issn.1673-0860.2019.04.008.
Wang H, Wu J, Zhang RX. [The Effect of NK-1R-siRNA On Expression of Inflammatory Factors in Allergic Rhinitis]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2019 Apr 7;54(4):280-285. PubMed PMID: 30991778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The effect of NK-1R-siRNA on expression of inflammatory factors in allergic rhinitis]. AU - Wang,H, AU - Wu,J, AU - Zhang,R X, PY - 2019/4/18/entrez PY - 2019/4/18/pubmed PY - 2019/4/24/medline KW - Inflammation factors KW - NK-1R-siRNA KW - Rhinitis, allergic SP - 280 EP - 285 JF - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery JO - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi VL - 54 IS - 4 N2 - Objective: To explore the effect of neurokinin-1 receptor small interfering RNA (NK-1R-siRNA) on the expression of inflammation factors in allergic rhinitis (AR). Methods: Twenty-four male SD rats were divided into three groups randomly (by random number table methord): NK-1R-siRNA group, negative control siRNA (NC-siRNA) group and saline group, with 8 rats in each group. SD rats were sensitized and challenged with ovalbumin (OVA) to induce AR. The rats were treated intranasally with NK-1R-siRNA, NC-siRNA or saline before and during the challenge period. The AR symptoms were observed. The levels of OVA-specific IgE were measured by enzyme-linked immunosorbent assay (ELISA). The levels of NK-1R expression in the nasal mucosal tissues were determined by real time PCR (RT-PCR) and immunohistochemistry. Antibody array was used in studying the expression of inflammation cell factors in nasal mucosa. SPSS 11.0 software was used for one-factor analysis of variance. Results: Compared with saline group, AR symptoms relived significantly in NK-1R-siRNA group (nose rubbing (31.4±8.9)/15 min vs (69.5±17.9)/15 min, sneezing (7.2±1.9)/15 min vs (23.7±9.2)/15 min, nasal secretions (7.1±2.3) mg vs (24.1±4.4) mg, t value was 38.100, 17.125, 16.837, respectively, all P<0.01), and the level of serum OVA-specific IgE was also reduced ((8.56±0.73) ng/ml vs (18.05±1.22) ng/ml, t=9.787, P<0.01). The RT-PCR and immunohistochemistry results showed that the expression of NK-1R in nasal mucosa of NK-1R-siRNA group was remarkably reduced than that of the NC-siRNA group and saline group. After the treatment of NK-1R-siRNA, the expression of interleukin (IL) 1α, IL-1β, IL-4, IL-6 and IL-13 decreased, while the interferon-γ (IFN-γ) and IL-10 increased. Conclusion: NK-1R-siRNA could regulate the release of inflammation factors in AR nasal mucosa, thus relive the allergic inflammation. SN - 1673-0860 UR - https://www.unboundmedicine.com/medline/citation/30991778/[The_effect_of_NK_1R_siRNA_on_expression_of_inflammatory_factors_in_allergic_rhinitis]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1673-0860&amp;year=2019&amp;vol=54&amp;issue=4&amp;fpage=280 DB - PRIME DP - Unbound Medicine ER -