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Starvation-induced autophagy is up-regulated via ROS-mediated ClC-3 chloride channel activation in the nasopharyngeal carcinoma cell line CNE-2Z.
Biochem J 2019; 476(9):1323-1333BJ

Abstract

Nutrient deficiency develops frequently in nasopharyngeal carcinoma cell (CNE-2Z) due to the characteristics of aggregation and uncontrolled proliferation. Therefore, starvation can induce autophagy in these cells. Chloride channel 3 (ClC-3), a member of the chloride channel family, is involved in various biological processes. However, whether ClC-3 plays an important role in starvation-induced autophagy is unclear. In this study, Earle's balanced salt solution (EBSS) was used to induce autophagy in CNE-2Z cells. We found that autophagy and the chloride current induced by EBSS were inhibited by chloride channel blockers. ClC-3 knockdown inhibited the degradation of LC3-II and P62. Furthermore, when reactive oxygen species (ROS) generation was suppressed by antioxidant N-acetyl-l-cysteine (L-NAC) pretreatment, EBSS-induced autophagy was inhibited, and the chloride current was unable to be activated. Nevertheless, ClC-3 knockdown had little effect on ROS levels, indicating that ROS acted upstream of ClC-3 and that both ROS and ClC-3 participated in EBSS-induced autophagy regulation in CNE-2Z.

Authors+Show Affiliations

Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China. Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China. Department of Pathophysiology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China. Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China. Department of Pathophysiology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China. Department of Pathophysiology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China. Department of Pathophysiology, Medical College, Jinan University, Guangzhou 510632, China.Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China.Department of Breast Surgery, The First People's Hospital of Foshan, Foshan 528000, China.Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China.Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3AX, Wales, U.K.Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China pengshuang@jnu.edu.cn tchenlixin@jnu.edu.cn twangliwei@jnu.edu.cn.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China pengshuang@jnu.edu.cn tchenlixin@jnu.edu.cn twangliwei@jnu.edu.cn.Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China pengshuang@jnu.edu.cn tchenlixin@jnu.edu.cn twangliwei@jnu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30992317

Citation

Zheng, Yanfang, et al. "Starvation-induced Autophagy Is Up-regulated Via ROS-mediated ClC-3 Chloride Channel Activation in the Nasopharyngeal Carcinoma Cell Line CNE-2Z." The Biochemical Journal, vol. 476, no. 9, 2019, pp. 1323-1333.
Zheng Y, Chen Z, Gu Z, et al. Starvation-induced autophagy is up-regulated via ROS-mediated ClC-3 chloride channel activation in the nasopharyngeal carcinoma cell line CNE-2Z. Biochem J. 2019;476(9):1323-1333.
Zheng, Y., Chen, Z., Gu, Z., Yang, X., Yu, M., Zhao, C., ... Wang, L. (2019). Starvation-induced autophagy is up-regulated via ROS-mediated ClC-3 chloride channel activation in the nasopharyngeal carcinoma cell line CNE-2Z. The Biochemical Journal, 476(9), pp. 1323-1333. doi:10.1042/BCJ20180979.
Zheng Y, et al. Starvation-induced Autophagy Is Up-regulated Via ROS-mediated ClC-3 Chloride Channel Activation in the Nasopharyngeal Carcinoma Cell Line CNE-2Z. Biochem J. 2019 May 7;476(9):1323-1333. PubMed PMID: 30992317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Starvation-induced autophagy is up-regulated via ROS-mediated ClC-3 chloride channel activation in the nasopharyngeal carcinoma cell line CNE-2Z. AU - Zheng,Yanfang, AU - Chen,Zhanru, AU - Gu,Zhuoyu, AU - Yang,Xiaoya, AU - Yu,Meisheng, AU - Zhao,Chan, AU - Lin,Jiawei, AU - Xu,Peisheng, AU - Zhu,Linyan, AU - Jacob,Tim J C, AU - Peng,Shuang, AU - Chen,Lixin, AU - Wang,Liwei, Y1 - 2019/05/07/ PY - 2018/12/31/received PY - 2019/04/13/revised PY - 2019/04/16/accepted PY - 2019/4/18/pubmed PY - 2019/4/18/medline PY - 2019/4/18/entrez KW - CLC-3 KW - ROS KW - autophagy KW - nasopharyngeal carcinoma KW - starvation SP - 1323 EP - 1333 JF - The Biochemical journal JO - Biochem. J. VL - 476 IS - 9 N2 - Nutrient deficiency develops frequently in nasopharyngeal carcinoma cell (CNE-2Z) due to the characteristics of aggregation and uncontrolled proliferation. Therefore, starvation can induce autophagy in these cells. Chloride channel 3 (ClC-3), a member of the chloride channel family, is involved in various biological processes. However, whether ClC-3 plays an important role in starvation-induced autophagy is unclear. In this study, Earle's balanced salt solution (EBSS) was used to induce autophagy in CNE-2Z cells. We found that autophagy and the chloride current induced by EBSS were inhibited by chloride channel blockers. ClC-3 knockdown inhibited the degradation of LC3-II and P62. Furthermore, when reactive oxygen species (ROS) generation was suppressed by antioxidant N-acetyl-l-cysteine (L-NAC) pretreatment, EBSS-induced autophagy was inhibited, and the chloride current was unable to be activated. Nevertheless, ClC-3 knockdown had little effect on ROS levels, indicating that ROS acted upstream of ClC-3 and that both ROS and ClC-3 participated in EBSS-induced autophagy regulation in CNE-2Z. SN - 1470-8728 UR - https://www.unboundmedicine.com/medline/citation/30992317/Starvation_induced_autophagy_is_up_regulated_via_ROS_mediated_ClC_3_chloride_channel_activation_in_the_nasopharyngeal_carcinoma_cell_line_CNE_2Z_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/BCJ20180979 DB - PRIME DP - Unbound Medicine ER -