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NT5E is associated with unfavorable prognosis and regulates cell proliferation and motility in gastric cancer.

Abstract

Ecto-5'-nucleotidase (NT5E) is a glycosylphosphatidylinositol anchored cell surface protein, and has been suggested to be dysregulated in most types of human cancer including gastric cancer. The aim of the present study was to present more evidence about the clinical and prognostic value of Ecto-5'-nucleotidase in gastric cancer patients, and preliminarily explore the biological function of Ecto-5'-nucleotidase in gastric cancer cells. In our study, high Ecto-5'-nucleotidase expression was observed in gastric cancer tissues and cell lines, respectively, compared with normal gastric mucosa tissues cells. Meanwhile, TCGA database also indicated that Ecto-5'-nucleotidase expression levels were notably elevated in gastric cancer tissues compared with normal gastric mucosa tissues. Furthermore, high-expression of Ecto-5'-nucleotidase was obviously associated with advanced clinical stage, deep tumor invasion, lymph node metastasis and distant metastasis in gastric cancer patients. The survival analyses of TCGA database and our study consistent suggested high Ecto-5'-nucleotidase expression was negatively correlated with overall survival time in gastric cancer patients. The univariate and multivariate Cox proportional hazards regression model showed high Ecto-5'-nucleotidase expression was an independent poor prognostic factor for gastric cancer patients. Moreover, silencing of Ecto-5'-nucleotidase expression suppressed cell proliferation, migration and invasion in vitro in gastric cancer. In conclusion, Ecto-5'-nucleotidase is a credible prognostic biomarker, and serves as a potential therapeutic target in gastric cancer.

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  • Authors+Show Affiliations

    ,

    Department of General Surgery, Zhoucheng People's Hospital, Zhoucheng 27350012, Shandong, China.

    ,

    Department of General Surgery, Zhoucheng People's Hospital, Zhoucheng 27350012, Shandong, China.

    ,

    Department of General Surgery, Zhoucheng People's Hospital, Zhoucheng 27350012, Shandong, China yinxiankundr@sina.com.

    ,

    Department of Urology, The Affiliated Hospital of Jining Medical College, Jining 272029, Shandong, China.

    Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250000, Shandong, China.

    Source

    Bioscience reports 39:5 2019 May 31 pg

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30992388

    Citation

    Hu, Sifeng, et al. "NT5E Is Associated With Unfavorable Prognosis and Regulates Cell Proliferation and Motility in Gastric Cancer." Bioscience Reports, vol. 39, no. 5, 2019.
    Hu S, Meng F, Yin X, et al. NT5E is associated with unfavorable prognosis and regulates cell proliferation and motility in gastric cancer. Biosci Rep. 2019;39(5).
    Hu, S., Meng, F., Yin, X., Cao, C., & Zhang, G. (2019). NT5E is associated with unfavorable prognosis and regulates cell proliferation and motility in gastric cancer. Bioscience Reports, 39(5), doi:10.1042/BSR20190101.
    Hu S, et al. NT5E Is Associated With Unfavorable Prognosis and Regulates Cell Proliferation and Motility in Gastric Cancer. Biosci Rep. 2019 May 31;39(5) PubMed PMID: 30992388.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - NT5E is associated with unfavorable prognosis and regulates cell proliferation and motility in gastric cancer. AU - Hu,Sifeng, AU - Meng,Fanmei, AU - Yin,Xiankun, AU - Cao,Changling, AU - Zhang,Guangyong, Y1 - 2019/05/17/ PY - 2019/01/11/received PY - 2019/03/19/revised PY - 2019/04/01/accepted PY - 2019/4/18/pubmed PY - 2019/4/18/medline PY - 2019/4/18/entrez KW - NT5E KW - biomarkers KW - gastric cancer KW - prognosis JF - Bioscience reports JO - Biosci. Rep. VL - 39 IS - 5 N2 - Ecto-5'-nucleotidase (NT5E) is a glycosylphosphatidylinositol anchored cell surface protein, and has been suggested to be dysregulated in most types of human cancer including gastric cancer. The aim of the present study was to present more evidence about the clinical and prognostic value of Ecto-5'-nucleotidase in gastric cancer patients, and preliminarily explore the biological function of Ecto-5'-nucleotidase in gastric cancer cells. In our study, high Ecto-5'-nucleotidase expression was observed in gastric cancer tissues and cell lines, respectively, compared with normal gastric mucosa tissues cells. Meanwhile, TCGA database also indicated that Ecto-5'-nucleotidase expression levels were notably elevated in gastric cancer tissues compared with normal gastric mucosa tissues. Furthermore, high-expression of Ecto-5'-nucleotidase was obviously associated with advanced clinical stage, deep tumor invasion, lymph node metastasis and distant metastasis in gastric cancer patients. The survival analyses of TCGA database and our study consistent suggested high Ecto-5'-nucleotidase expression was negatively correlated with overall survival time in gastric cancer patients. The univariate and multivariate Cox proportional hazards regression model showed high Ecto-5'-nucleotidase expression was an independent poor prognostic factor for gastric cancer patients. Moreover, silencing of Ecto-5'-nucleotidase expression suppressed cell proliferation, migration and invasion in vitro in gastric cancer. In conclusion, Ecto-5'-nucleotidase is a credible prognostic biomarker, and serves as a potential therapeutic target in gastric cancer. SN - 1573-4935 UR - https://www.unboundmedicine.com/medline/citation/30992388/NT5E_is_associated_with_unfavorable_prognosis_and_regulates_cell_proliferation_and_motility_in_gastric_cancer L2 - http://www.bioscirep.org/cgi/pmidlookup?view=long&pmid=30992388 DB - PRIME DP - Unbound Medicine ER -