Tags

Type your tag names separated by a space and hit enter

Evaluation of the Degree of Polymerization of the Proanthocyanidins in Cranberry by Molecular Sieving and Characterization of the Low Molecular Weight Fractions by UHPLC-Orbitrap Mass Spectrometry.
Molecules 2019; 24(8)M

Abstract

4-dimethylammino-cinnamaldehyde (DMAC) assays quantify total proanthocyanidins (PACs) but do not provide qualitative PAC molecular weight distribution information and cannot discriminate between A- and B-type PACs. We developed an efficient method for assessing PAC molecular weight distributions. The PACs from three commercial cranberry extracts (A1-A3) were fractionated by molecular sieves with cut-offs of 3, 10, 30, 50, and 100 kDa, and each fraction was analyzed by DMAC assays. A1, A2, and A3 contained 27%, 33%, and 15% PACs, respectively. Approximately 28 PACs, 20 flavonols, and 15 phenolic acids were identified by UHPLC-DAD-Orbitrap MS in A1 and A3, while A2 contained only flavan-3-ols. Epicatechin was the main monomer in A1 and A3, and catechin was the main in A2. Procyanidin A2 was the main dimer in A1 and A3, representing more than 85% of the total dimers, while it constituted approximately only 24% of A2. A1 and A3 contained quercetin, isorhamnetin, myricetin, and their glycosides, which were totally absent in A2. In A1 and A3 the PACs were mainly distributed in the fractions 30-3 and <3 kDa, while in A2 more than 70% were present in the fraction less than 3 kDa. Overall, obtained data strongly suggests that A2 is not cranberry-derived, or is adulterated with another source of PACs.

Authors+Show Affiliations

DeFENS-Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy. claudio.gardana@unimi.it.DeFENS-Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy. paolo.simonetti@unimi.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30999600

Citation

Gardana, Claudio, and Paolo Simonetti. "Evaluation of the Degree of Polymerization of the Proanthocyanidins in Cranberry By Molecular Sieving and Characterization of the Low Molecular Weight Fractions By UHPLC-Orbitrap Mass Spectrometry." Molecules (Basel, Switzerland), vol. 24, no. 8, 2019.
Gardana C, Simonetti P. Evaluation of the Degree of Polymerization of the Proanthocyanidins in Cranberry by Molecular Sieving and Characterization of the Low Molecular Weight Fractions by UHPLC-Orbitrap Mass Spectrometry. Molecules. 2019;24(8).
Gardana, C., & Simonetti, P. (2019). Evaluation of the Degree of Polymerization of the Proanthocyanidins in Cranberry by Molecular Sieving and Characterization of the Low Molecular Weight Fractions by UHPLC-Orbitrap Mass Spectrometry. Molecules (Basel, Switzerland), 24(8), doi:10.3390/molecules24081504.
Gardana C, Simonetti P. Evaluation of the Degree of Polymerization of the Proanthocyanidins in Cranberry By Molecular Sieving and Characterization of the Low Molecular Weight Fractions By UHPLC-Orbitrap Mass Spectrometry. Molecules. 2019 Apr 17;24(8) PubMed PMID: 30999600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the Degree of Polymerization of the Proanthocyanidins in Cranberry by Molecular Sieving and Characterization of the Low Molecular Weight Fractions by UHPLC-Orbitrap Mass Spectrometry. AU - Gardana,Claudio, AU - Simonetti,Paolo, Y1 - 2019/04/17/ PY - 2019/03/27/received PY - 2019/04/09/revised PY - 2019/04/15/accepted PY - 2019/4/20/entrez PY - 2019/4/20/pubmed PY - 2019/8/7/medline KW - DMAC assay KW - UHPLC-DAD-Orbitrap MS KW - Vaccinium macrocarpon KW - molecular sieve KW - proanthocyanidins JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 8 N2 - 4-dimethylammino-cinnamaldehyde (DMAC) assays quantify total proanthocyanidins (PACs) but do not provide qualitative PAC molecular weight distribution information and cannot discriminate between A- and B-type PACs. We developed an efficient method for assessing PAC molecular weight distributions. The PACs from three commercial cranberry extracts (A1-A3) were fractionated by molecular sieves with cut-offs of 3, 10, 30, 50, and 100 kDa, and each fraction was analyzed by DMAC assays. A1, A2, and A3 contained 27%, 33%, and 15% PACs, respectively. Approximately 28 PACs, 20 flavonols, and 15 phenolic acids were identified by UHPLC-DAD-Orbitrap MS in A1 and A3, while A2 contained only flavan-3-ols. Epicatechin was the main monomer in A1 and A3, and catechin was the main in A2. Procyanidin A2 was the main dimer in A1 and A3, representing more than 85% of the total dimers, while it constituted approximately only 24% of A2. A1 and A3 contained quercetin, isorhamnetin, myricetin, and their glycosides, which were totally absent in A2. In A1 and A3 the PACs were mainly distributed in the fractions 30-3 and <3 kDa, while in A2 more than 70% were present in the fraction less than 3 kDa. Overall, obtained data strongly suggests that A2 is not cranberry-derived, or is adulterated with another source of PACs. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/30999600/Evaluation_of_the_Degree_of_Polymerization_of_the_Proanthocyanidins_in_Cranberry_by_Molecular_Sieving_and_Characterization_of_the_Low_Molecular_Weight_Fractions_by_UHPLC_Orbitrap_Mass_Spectrometry_ L2 - http://www.mdpi.com/resolver?pii=molecules24081504 DB - PRIME DP - Unbound Medicine ER -