Tags

Type your tag names separated by a space and hit enter

In Vivo Immunological Effects of CD73 Deficiency.
Cell Physiol Biochem 2019; 52(5):1192-1202CP

Abstract

BACKGROUND/AIMS

The extracellular ecto-5'-nucleotidase (CD73) is involved in the production of immunosuppressive adenosin (Ado), which can influence different immune cells through the specific adenosine receptors. The main aim of this work was to characterize immune cell populations as well as serum cytokine level in healthy CD73-deficient mice compared to healthy wild-type animals.

METHODS

Profound immnophenotyping of splenocytes from healthy CD73-deficient and wild-type mice was done using flow cytometry (FACS analysis). Cytokine measurement in the serum of the animals was carried out with a Bio-Plex assay.

RESULTS

The CD73-deficience leads to an increase in a percentage of NK cells and pDC, as well as influences expression of the costimulatory molecules CD80 and CD86. The knockout mice in opposite to wild-type animals show high amount of effector CD4+ T-cells in the spleens. No changes have been found in the subpopulations of CD8+ T-cells. Besides, CD73-deficience leads to a decrease in the percentage of regulatory T cells. Compared with the wild-type animals we found that CD73 knockout mice possess low serum concentration of IL-6.

CONCLUSION

This in vivo study clear demonstrated certain immunological changes in the CD73-deficient mice and thus immunoregulatory potential of CD73 molecule. This makes this extracellular enzyme to a real immune check point molecule, attractive for further investigations and clinical studies.

Authors+Show Affiliations

Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Munich, Germany.Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Munich, Germany. Sana Klinikum Offenbach, Offenbach, Germany.Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Munich, Germany. Department of Histology, Embryology and Cytology, Azerbaijan Medical University, Baku, Azerbaijan.Department of Cell Signalling, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Munich, Germany. German Cancer Consortium (DKTK), Partner Site Munich, Munich, Germany.Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany. Section Surgical Research, University of Heidelberg, Heidelberg, Germany.Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Munich, Germany. German Cancer Consortium (DKTK), Partner Site Munich, Munich, Germany, alexandr.bazhin@med.uni-muenchen.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31001959

Citation

Adam, Thomas, et al. "In Vivo Immunological Effects of CD73 Deficiency." Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, vol. 52, no. 5, 2019, pp. 1192-1202.
Adam T, Mathes A, Isayev O, et al. In Vivo Immunological Effects of CD73 Deficiency. Cell Physiol Biochem. 2019;52(5):1192-1202.
Adam, T., Mathes, A., Isayev, O., Philippov, P. P., Werner, J., Karakhanova, S., & Bazhin, A. V. (2019). In Vivo Immunological Effects of CD73 Deficiency. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 52(5), pp. 1192-1202. doi:10.33594/000000081.
Adam T, et al. In Vivo Immunological Effects of CD73 Deficiency. Cell Physiol Biochem. 2019;52(5):1192-1202. PubMed PMID: 31001959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In Vivo Immunological Effects of CD73 Deficiency. AU - Adam,Thomas, AU - Mathes,Andreas, AU - Isayev,Orkhan, AU - Philippov,Pavel P, AU - Werner,Jens, AU - Karakhanova,Svetlana, AU - Bazhin,Alexandr V, PY - 2018/12/21/received PY - 2019/04/03/accepted PY - 2019/4/20/entrez PY - 2019/4/20/pubmed PY - 2019/4/20/medline KW - Cd73 KW - Immune regulation KW - Immune regulatory molecules KW - Knockout mice SP - 1192 EP - 1202 JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JO - Cell. Physiol. Biochem. VL - 52 IS - 5 N2 - BACKGROUND/AIMS: The extracellular ecto-5'-nucleotidase (CD73) is involved in the production of immunosuppressive adenosin (Ado), which can influence different immune cells through the specific adenosine receptors. The main aim of this work was to characterize immune cell populations as well as serum cytokine level in healthy CD73-deficient mice compared to healthy wild-type animals. METHODS: Profound immnophenotyping of splenocytes from healthy CD73-deficient and wild-type mice was done using flow cytometry (FACS analysis). Cytokine measurement in the serum of the animals was carried out with a Bio-Plex assay. RESULTS: The CD73-deficience leads to an increase in a percentage of NK cells and pDC, as well as influences expression of the costimulatory molecules CD80 and CD86. The knockout mice in opposite to wild-type animals show high amount of effector CD4+ T-cells in the spleens. No changes have been found in the subpopulations of CD8+ T-cells. Besides, CD73-deficience leads to a decrease in the percentage of regulatory T cells. Compared with the wild-type animals we found that CD73 knockout mice possess low serum concentration of IL-6. CONCLUSION: This in vivo study clear demonstrated certain immunological changes in the CD73-deficient mice and thus immunoregulatory potential of CD73 molecule. This makes this extracellular enzyme to a real immune check point molecule, attractive for further investigations and clinical studies. SN - 1421-9778 UR - https://www.unboundmedicine.com/medline/citation/31001959/In_Vivo_Immunological_Effects_of_CD73_Deficiency L2 - https://www.cellphysiolbiochem.com/Articles/?DOI=10.33594/000000081 DB - PRIME DP - Unbound Medicine ER -