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Highly expressed microRNA-940 promotes early abortion by regulating placenta implantation by targeting ZNF672.

Abstract

OBJECTIVE

Our study aims to explore whether microRNA-940 could participate in early abortion by inhibiting placenta implantation and its underlying mechanism.

PATIENTS AND METHODS

Expressions of microRNA-940 and ZNF672 in the placental villi of 6 early abortion pregnancies and 6 normal pregnancies were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Expressions of microRNA-940 and ZNF672 in trophoblast cells (BeWo, JEG3, Wish and HTR-8) were also detected. Cell counting kit-8 (CCK-8) assay was performed to detect the effect of microRNA-940 on the proliferation of trophoblast cells after being transfected with microRNA-940 inhibitor or mimics, respectively. Dual-Luciferase reporter gene assay and RNA binding protein immunoprecipitation (RIP) assay were conducted to demonstrate the binding condition of microRNA-940 to ZNF672.

RESULTS

MicroRNA-940 was highly expressed in placental villi of early abortion pregnancies, whereas ZNF672 was lowly expressed. HTR-8 cells expressed the highest level and BeWo cells expressed the lowest level of microRNA-940. After transfection of microRNA-940 inhibitor in HTR-8 cells, the proliferative capacity was remarkably increased. On the contrary, the transfection of microRNA-940 mimic downregulated the proliferation of BeWo cells. Dual-Luciferase reporter gene assay demonstrated that microRNA-940 targets ZNF672. RIP results further indicated that microRNA-940 binds to ZNF672.

CONCLUSIONS

MicroRNA-940 is highly expressed in the placental villi of early abortion pregnancies and promotes the occurrence of early abortion by inhibiting the proliferation of trophoblast cells by targeting ZNF672.

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  • Authors+Show Affiliations

    ,

    Department of Obstetrics, Xinchang People Hospital, Xinchang, China. 1947562381@qq.com.

    , , ,

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31002163

    Citation

    Zhang, C-Y, et al. "Highly Expressed microRNA-940 Promotes Early Abortion By Regulating Placenta Implantation By Targeting ZNF672." European Review for Medical and Pharmacological Sciences, vol. 23, no. 7, 2019, pp. 2693-2700.
    Zhang CY, Lu XL, Yang Y, et al. Highly expressed microRNA-940 promotes early abortion by regulating placenta implantation by targeting ZNF672. Eur Rev Med Pharmacol Sci. 2019;23(7):2693-2700.
    Zhang, C. Y., Lu, X. L., Yang, Y., Ayi, N., & Li, L. H. (2019). Highly expressed microRNA-940 promotes early abortion by regulating placenta implantation by targeting ZNF672. European Review for Medical and Pharmacological Sciences, 23(7), pp. 2693-2700. doi:10.26355/eurrev_201904_17539.
    Zhang CY, et al. Highly Expressed microRNA-940 Promotes Early Abortion By Regulating Placenta Implantation By Targeting ZNF672. Eur Rev Med Pharmacol Sci. 2019;23(7):2693-2700. PubMed PMID: 31002163.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Highly expressed microRNA-940 promotes early abortion by regulating placenta implantation by targeting ZNF672. AU - Zhang,C-Y, AU - Lu,X-L, AU - Yang,Y, AU - Ayi,N, AU - Li,L-H, PY - 2019/4/20/entrez SP - 2693 EP - 2700 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 23 IS - 7 N2 - OBJECTIVE: Our study aims to explore whether microRNA-940 could participate in early abortion by inhibiting placenta implantation and its underlying mechanism. PATIENTS AND METHODS: Expressions of microRNA-940 and ZNF672 in the placental villi of 6 early abortion pregnancies and 6 normal pregnancies were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Expressions of microRNA-940 and ZNF672 in trophoblast cells (BeWo, JEG3, Wish and HTR-8) were also detected. Cell counting kit-8 (CCK-8) assay was performed to detect the effect of microRNA-940 on the proliferation of trophoblast cells after being transfected with microRNA-940 inhibitor or mimics, respectively. Dual-Luciferase reporter gene assay and RNA binding protein immunoprecipitation (RIP) assay were conducted to demonstrate the binding condition of microRNA-940 to ZNF672. RESULTS: MicroRNA-940 was highly expressed in placental villi of early abortion pregnancies, whereas ZNF672 was lowly expressed. HTR-8 cells expressed the highest level and BeWo cells expressed the lowest level of microRNA-940. After transfection of microRNA-940 inhibitor in HTR-8 cells, the proliferative capacity was remarkably increased. On the contrary, the transfection of microRNA-940 mimic downregulated the proliferation of BeWo cells. Dual-Luciferase reporter gene assay demonstrated that microRNA-940 targets ZNF672. RIP results further indicated that microRNA-940 binds to ZNF672. CONCLUSIONS: MicroRNA-940 is highly expressed in the placental villi of early abortion pregnancies and promotes the occurrence of early abortion by inhibiting the proliferation of trophoblast cells by targeting ZNF672. SN - 2284-0729 UR - https://www.unboundmedicine.com/medline/citation/31002163/Highly_expressed_microRNA-940_promotes_early_abortion_by_regulating_placenta_implantation_by_targeting_ZNF672 L2 - https://www.europeanreview.org/article/17539 DB - PRIME DP - Unbound Medicine ER -