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Quantitative proteomic analysis of venom from Southern India common krait (Bungarus caeruleus) and identification of poorly immunogenic toxins by immune-profiling against commercial antivenom.
Expert Rev Proteomics. 2019 05; 16(5):457-469.ER

Abstract

OBJECTIVES

To study the venom proteome composition of Southern India (SI) Common Krait (Bungarus caeruleus) and immunological cross-reactivity between venom against commercial antivenom.

METHODS

Proteomic analysis was done by nano LC-MS/MS and toxins were quantitated by label-free analysis. The immunological cross-reactivity of venom towards polyvalent antivenom (PAV) was assessed by ELISA, Immunoblotting, and immuno-chromatographic methods.

RESULTS

A total of 57 enzymatic and non-enzymatic proteins belonging to 12 snake venom protein families were identified. The three finger toxins (3FTx) (48.3%) and phospholipase A2 (PLA2) (37.6%) represented the most abundant non-enzymatic and enzymatic proteins, respectively. β-bungarotoxin (12.9%), a presynaptic neurotoxin, was also identified. The venom proteome composition is well correlated with its enzymatic activities, reported pharmacological properties, and clinical manifestations of krait envenomation. Immuno-cross-reactivity studies demonstrated better recognition of high molecular weight proteins (>45 kDa) of this venom by PAVs compared to low molecular weight (<15 kDa) toxins such as PLA2 and 3FTxs.

CONCLUSION

The poor recognition of <15 kDa mass SI B. caeruleus venom proteins is of grave concern for the successful treatment of krait envenomation. Therefore, emphasis should be given to improve the immunization protocols and/or supplement of antibodies raised specifically against the <15 kDa toxins of this venom.

Authors+Show Affiliations

a Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology , Tezpur University , Tezpur , India.a Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology , Tezpur University , Tezpur , India.a Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology , Tezpur University , Tezpur , India.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31002271

Citation

Patra, Aparup, et al. "Quantitative Proteomic Analysis of Venom From Southern India Common Krait (Bungarus Caeruleus) and Identification of Poorly Immunogenic Toxins By Immune-profiling Against Commercial Antivenom." Expert Review of Proteomics, vol. 16, no. 5, 2019, pp. 457-469.
Patra A, Chanda A, Mukherjee AK. Quantitative proteomic analysis of venom from Southern India common krait (Bungarus caeruleus) and identification of poorly immunogenic toxins by immune-profiling against commercial antivenom. Expert Rev Proteomics. 2019;16(5):457-469.
Patra, A., Chanda, A., & Mukherjee, A. K. (2019). Quantitative proteomic analysis of venom from Southern India common krait (Bungarus caeruleus) and identification of poorly immunogenic toxins by immune-profiling against commercial antivenom. Expert Review of Proteomics, 16(5), 457-469. https://doi.org/10.1080/14789450.2019.1609945
Patra A, Chanda A, Mukherjee AK. Quantitative Proteomic Analysis of Venom From Southern India Common Krait (Bungarus Caeruleus) and Identification of Poorly Immunogenic Toxins By Immune-profiling Against Commercial Antivenom. Expert Rev Proteomics. 2019;16(5):457-469. PubMed PMID: 31002271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative proteomic analysis of venom from Southern India common krait (Bungarus caeruleus) and identification of poorly immunogenic toxins by immune-profiling against commercial antivenom. AU - Patra,Aparup, AU - Chanda,Abhishek, AU - Mukherjee,Ashis K, Y1 - 2019/04/27/ PY - 2019/4/20/pubmed PY - 2020/6/13/medline PY - 2019/4/20/entrez KW - ESI-LC MS/MS KW - krait venom KW - pre-synaptic neurotoxin KW - snakebite KW - venom proteome KW - venom–antivenom cross-reactivity SP - 457 EP - 469 JF - Expert review of proteomics JO - Expert Rev Proteomics VL - 16 IS - 5 N2 - OBJECTIVES: To study the venom proteome composition of Southern India (SI) Common Krait (Bungarus caeruleus) and immunological cross-reactivity between venom against commercial antivenom. METHODS: Proteomic analysis was done by nano LC-MS/MS and toxins were quantitated by label-free analysis. The immunological cross-reactivity of venom towards polyvalent antivenom (PAV) was assessed by ELISA, Immunoblotting, and immuno-chromatographic methods. RESULTS: A total of 57 enzymatic and non-enzymatic proteins belonging to 12 snake venom protein families were identified. The three finger toxins (3FTx) (48.3%) and phospholipase A2 (PLA2) (37.6%) represented the most abundant non-enzymatic and enzymatic proteins, respectively. β-bungarotoxin (12.9%), a presynaptic neurotoxin, was also identified. The venom proteome composition is well correlated with its enzymatic activities, reported pharmacological properties, and clinical manifestations of krait envenomation. Immuno-cross-reactivity studies demonstrated better recognition of high molecular weight proteins (>45 kDa) of this venom by PAVs compared to low molecular weight (<15 kDa) toxins such as PLA2 and 3FTxs. CONCLUSION: The poor recognition of <15 kDa mass SI B. caeruleus venom proteins is of grave concern for the successful treatment of krait envenomation. Therefore, emphasis should be given to improve the immunization protocols and/or supplement of antibodies raised specifically against the <15 kDa toxins of this venom. SN - 1744-8387 UR - https://www.unboundmedicine.com/medline/citation/31002271/Quantitative_proteomic_analysis_of_venom_from_Southern_India_common_krait__Bungarus_caeruleus__and_identification_of_poorly_immunogenic_toxins_by_immune_profiling_against_commercial_antivenom_ L2 - http://www.tandfonline.com/doi/full/10.1080/14789450.2019.1609945 DB - PRIME DP - Unbound Medicine ER -