Tags

Type your tag names separated by a space and hit enter

Genome-wide identification of ATP-binding cassette transporters and expression profiles in the Asian citrus psyllid, Diaphorina citri, exposed to imidacloprid.
Comp Biochem Physiol Part D Genomics Proteomics. 2019 06; 30:305-311.CB

Abstract

ATP-binding cassette (ABC) transporters belong to a large protein superfamily found in both prokaryotic and eukaryotic organisms. In arthropods, ABC transporters are involved in defense and resistance to insecticides. The Asian citrus psyllid (ACP), Diaphorina citri, is a major vector of the Huanglongbing pathogen (Candidatus Liberibacter asiaticus) worldwide. In this study, the ABC transporter genes were identified based on the transcriptome and genome database of D. citri. A total of 44 DcitABC transporters were identified and grouped into 8 subfamilies (ABCA-ABCH), including 4 DcitABCAs, 4 Bs, 5 Cs, 2 Ds, 1 E, 3 Fs, 15 Gs and 10 Hs, respectively. Phylogenetic analysis of ABC transporters revealed a close relationship between D. citri and Laodelphax striatellus. qPCR analyses showed that several DcitABC transporters were highly expressed in fat body, midgut and the hindgut with Malpighian tubules. In particular, DcitABCG11 and DcitABCG14 were most highly expressed in the hindgut. The transcript abundance of 11 DcitABC genes was significantly upregulated upon exposure to an LC50 concentration of imidacloprid. The expressions of three cytochrome P450 genes and one glutathione S-transferase gene were also significantly elevated. This suggested that DcitABC genes, together with metabolic enzyme genes, are associated with imidacloprid detoxification.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Liu XQ
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Jiang HB
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Xiong Y
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Peng P
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Li HF
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Yuan GR
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Dou W
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Wang JJ
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China. Electronic address: wangjinjun@swu.edu.cn.

MeSH

ATP-Binding Cassette TransportersAnimalsHemipteraInsect ProteinsInsecticide ResistanceInsecticidesNeonicotinoidsNitro CompoundsPhylogenyTranscriptomeUp-Regulation

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31004937

Citation

Liu, Xiao-Qiang, et al. "Genome-wide Identification of ATP-binding Cassette Transporters and Expression Profiles in the Asian Citrus Psyllid, Diaphorina Citri, Exposed to Imidacloprid." Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, vol. 30, 2019, pp. 305-311.
Liu XQ, Jiang HB, Xiong Y, et al. Genome-wide identification of ATP-binding cassette transporters and expression profiles in the Asian citrus psyllid, Diaphorina citri, exposed to imidacloprid. Comp Biochem Physiol Part D Genomics Proteomics. 2019;30:305-311.
Liu, X. Q., Jiang, H. B., Xiong, Y., Peng, P., Li, H. F., Yuan, G. R., Dou, W., & Wang, J. J. (2019). Genome-wide identification of ATP-binding cassette transporters and expression profiles in the Asian citrus psyllid, Diaphorina citri, exposed to imidacloprid. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, 30, 305-311. https://doi.org/10.1016/j.cbd.2019.04.003
Liu XQ, et al. Genome-wide Identification of ATP-binding Cassette Transporters and Expression Profiles in the Asian Citrus Psyllid, Diaphorina Citri, Exposed to Imidacloprid. Comp Biochem Physiol Part D Genomics Proteomics. 2019;30:305-311. PubMed PMID: 31004937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide identification of ATP-binding cassette transporters and expression profiles in the Asian citrus psyllid, Diaphorina citri, exposed to imidacloprid. AU - Liu,Xiao-Qiang, AU - Jiang,Hong-Bo, AU - Xiong,Ying, AU - Peng,Peng, AU - Li,Hong-Fei, AU - Yuan,Guo-Rui, AU - Dou,Wei, AU - Wang,Jin-Jun, Y1 - 2019/04/11/ PY - 2019/01/30/received PY - 2019/04/07/revised PY - 2019/04/08/accepted PY - 2019/4/21/pubmed PY - 2019/12/24/medline PY - 2019/4/21/entrez KW - ABC transporters KW - Detoxification KW - Huanglongbing vector KW - Imidacloprid induction KW - Transcriptional expression SP - 305 EP - 311 JF - Comparative biochemistry and physiology. Part D, Genomics & proteomics JO - Comp Biochem Physiol Part D Genomics Proteomics VL - 30 N2 - ATP-binding cassette (ABC) transporters belong to a large protein superfamily found in both prokaryotic and eukaryotic organisms. In arthropods, ABC transporters are involved in defense and resistance to insecticides. The Asian citrus psyllid (ACP), Diaphorina citri, is a major vector of the Huanglongbing pathogen (Candidatus Liberibacter asiaticus) worldwide. In this study, the ABC transporter genes were identified based on the transcriptome and genome database of D. citri. A total of 44 DcitABC transporters were identified and grouped into 8 subfamilies (ABCA-ABCH), including 4 DcitABCAs, 4 Bs, 5 Cs, 2 Ds, 1 E, 3 Fs, 15 Gs and 10 Hs, respectively. Phylogenetic analysis of ABC transporters revealed a close relationship between D. citri and Laodelphax striatellus. qPCR analyses showed that several DcitABC transporters were highly expressed in fat body, midgut and the hindgut with Malpighian tubules. In particular, DcitABCG11 and DcitABCG14 were most highly expressed in the hindgut. The transcript abundance of 11 DcitABC genes was significantly upregulated upon exposure to an LC50 concentration of imidacloprid. The expressions of three cytochrome P450 genes and one glutathione S-transferase gene were also significantly elevated. This suggested that DcitABC genes, together with metabolic enzyme genes, are associated with imidacloprid detoxification. SN - 1878-0407 UR - https://www.unboundmedicine.com/medline/citation/31004937/Genome_wide_identification_of_ATP_binding_cassette_transporters_and_expression_profiles_in_the_Asian_citrus_psyllid_Diaphorina_citri_exposed_to_imidacloprid_ DB - PRIME DP - Unbound Medicine ER -