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Comparison of Two Thrombin Generation Methods, CAT and ST-Genesia, in Liver Transplant Patients.
Thromb Haemost. 2019 Jun; 119(6):899-905.TH

Abstract

BACKGROUND

During liver transplantation (LT), thrombin generation (TG) is altered. The most frequently used assay for TG is the Calibrated Automated Thrombogram (CAT). It is designed for series of plasmas and is semi-automated. Complete automation has led to a new device, the ST-Genesia, enabling quantitative standardized TG evaluation.

OBJECTIVE

The aim of this observational study was to compare the TG results of the CAT and the ST-Genesia on frozen-thawed plasma samples prepared from the blood of LT patients.

PATIENTS AND METHODS

Poor platelet plasma aliquots were prepared from blood samples from six LT patients selected to get the whole range of TG and were assessed with CAT (recombinant human tissue factor [TF] concentration 5 pm) and with ST-Genesia Bleedscreen assay (BS, using 'low' recombinant human TF concentration) and Thromboscreen assay (TS, using 'medium' recombinant human TF concentration). The TG parameters studied were: lag time, peak, time to peak, endogenous thrombin potential, velocity index and start tail.

RESULTS

BS and TS did not differ significantly from each other whatever the parameter studied, whereas most of the CAT parameters were significantly different from those obtained with BS and TS. Hierarchical clustering analysis of the different parameters of TG showed three homogeneous groups. One cluster gathered TG quantitative parameters from ST-Genesia. A second cluster gathered all the kinetic parameters. The last cluster isolated the quantitative parameters of CAT.

CONCLUSION

In patients undergoing LT, TG performed with CAT and with ST-Genesia provided different results, for unknown reasons.

Authors+Show Affiliations

CHU Bordeaux, Service d'Anesthésie-Réanimation Pellegrin, Bordeaux, France. Université de Bordeaux, INSERM U 12-11, Maladies rares: Génétique et Métabolisme, Bordeaux, France.CHU Bordeaux, Laboratoire d'Hématologie - PTRR, Hôpital Pellegrin, Bordeaux, France. Université de Bordeaux, INSERM U 10-34, Biologie des Maladies Cardio-Vasculaires, Pessac, France.CHU Bordeaux, Laboratoire d'Hématologie - PTRR, Hôpital Pellegrin, Bordeaux, France.

Pub Type(s)

Clinical Trial
Journal Article
Observational Study

Language

eng

PubMed ID

31005063

Citation

Roullet, Stéphanie, et al. "Comparison of Two Thrombin Generation Methods, CAT and ST-Genesia, in Liver Transplant Patients." Thrombosis and Haemostasis, vol. 119, no. 6, 2019, pp. 899-905.
Roullet S, Labrouche S, Freyburger G. Comparison of Two Thrombin Generation Methods, CAT and ST-Genesia, in Liver Transplant Patients. Thromb Haemost. 2019;119(6):899-905.
Roullet, S., Labrouche, S., & Freyburger, G. (2019). Comparison of Two Thrombin Generation Methods, CAT and ST-Genesia, in Liver Transplant Patients. Thrombosis and Haemostasis, 119(6), 899-905. https://doi.org/10.1055/s-0039-1685452
Roullet S, Labrouche S, Freyburger G. Comparison of Two Thrombin Generation Methods, CAT and ST-Genesia, in Liver Transplant Patients. Thromb Haemost. 2019;119(6):899-905. PubMed PMID: 31005063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of Two Thrombin Generation Methods, CAT and ST-Genesia, in Liver Transplant Patients. AU - Roullet,Stéphanie, AU - Labrouche,Sylvie, AU - Freyburger,Geneviève, Y1 - 2019/04/20/ PY - 2019/4/21/pubmed PY - 2019/4/21/medline PY - 2019/4/21/entrez SP - 899 EP - 905 JF - Thrombosis and haemostasis JO - Thromb. Haemost. VL - 119 IS - 6 N2 - BACKGROUND: During liver transplantation (LT), thrombin generation (TG) is altered. The most frequently used assay for TG is the Calibrated Automated Thrombogram (CAT). It is designed for series of plasmas and is semi-automated. Complete automation has led to a new device, the ST-Genesia, enabling quantitative standardized TG evaluation. OBJECTIVE: The aim of this observational study was to compare the TG results of the CAT and the ST-Genesia on frozen-thawed plasma samples prepared from the blood of LT patients. PATIENTS AND METHODS: Poor platelet plasma aliquots were prepared from blood samples from six LT patients selected to get the whole range of TG and were assessed with CAT (recombinant human tissue factor [TF] concentration 5 pm) and with ST-Genesia Bleedscreen assay (BS, using 'low' recombinant human TF concentration) and Thromboscreen assay (TS, using 'medium' recombinant human TF concentration). The TG parameters studied were: lag time, peak, time to peak, endogenous thrombin potential, velocity index and start tail. RESULTS: BS and TS did not differ significantly from each other whatever the parameter studied, whereas most of the CAT parameters were significantly different from those obtained with BS and TS. Hierarchical clustering analysis of the different parameters of TG showed three homogeneous groups. One cluster gathered TG quantitative parameters from ST-Genesia. A second cluster gathered all the kinetic parameters. The last cluster isolated the quantitative parameters of CAT. CONCLUSION: In patients undergoing LT, TG performed with CAT and with ST-Genesia provided different results, for unknown reasons. SN - 2567-689X UR - https://www.unboundmedicine.com/medline/citation/31005063/Comparison_of_Two_Thrombin_Generation_Methods_CAT_and_ST_Genesia_in_Liver_Transplant_Patients_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0039-1685452 DB - PRIME DP - Unbound Medicine ER -