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Immunochemical and functional analysis of HLA class II antigens induced by recombinant immune interferon on normal epidermal melanocytes.
J Immunol. 1987 Feb 15; 138(4):1310-6.JI

Abstract

The effect of recombinant immune interferon (IFN-gamma) on the expression and shedding of HLA antigens and of melanoma-associated antigens (MAA) by epidermal melanocytes was investigated by using serologic and immunochemical techniques. IFN-gamma enhances the expression and/or shedding of HLA class I antigens and of the cytoplasmic MAA defined by monoclonal antibody (MoAb) 465.12S and induces a slight reduction in the expression of the high m.w. melanoma-associated antigen (HMW-MAA). In agreement with the data in the literature, melanocytes incubated with IFN-gamma acquire HLA-DR, -DQ, and -DP antigens. Contrary to previous information in the literature, the effect is not restricted to HLA class II antigens, since IFN-gamma also induces the expression of the 96-kDa MAA recognized by MoAb CL203. The effect of IFN-gamma on HLA class II antigens and 96-kDa MAA is dose and time dependent and is specific, because recombinant leukocyte interferon affects the expression of neither type of antigen. In spite of the expression of HLA class II antigens, IFN-gamma-treated melanocytes do not acquire the ability to stimulate the proliferation of allogeneic lymphocytes. HLA-DR antigens are more susceptible to induction by IFN-gamma than HLA-DQ and -DP antigens, since the percentage of melanocytes acquiring HLA-DQ and -DP antigens is lower than that acquiring HLA-DR antigens. Furthermore, the dose of IFN-gamma is higher and the time of incubation is longer to induce HLA-DQ and -DP antigens than to induce HLA-DR antigens. The differential susceptibility of HLA-DR, -DQ, and -DP antigens as well as of melanocytes from various donors to the modulating effect of IFN-gamma may provide an explanation for the more frequent detection of HLA-DR than of HLA-DQ and -DP antigens in melanoma lesions and for the expression of HLA class II antigens by some, but not all, melanoma lesions.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3100635

Citation

Tsujisaki, M, et al. "Immunochemical and Functional Analysis of HLA Class II Antigens Induced By Recombinant Immune Interferon On Normal Epidermal Melanocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 138, no. 4, 1987, pp. 1310-6.
Tsujisaki M, Igarashi M, Sakaguchi K, et al. Immunochemical and functional analysis of HLA class II antigens induced by recombinant immune interferon on normal epidermal melanocytes. J Immunol. 1987;138(4):1310-6.
Tsujisaki, M., Igarashi, M., Sakaguchi, K., Eisinger, M., Herlyn, M., & Ferrone, S. (1987). Immunochemical and functional analysis of HLA class II antigens induced by recombinant immune interferon on normal epidermal melanocytes. Journal of Immunology (Baltimore, Md. : 1950), 138(4), 1310-6.
Tsujisaki M, et al. Immunochemical and Functional Analysis of HLA Class II Antigens Induced By Recombinant Immune Interferon On Normal Epidermal Melanocytes. J Immunol. 1987 Feb 15;138(4):1310-6. PubMed PMID: 3100635.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunochemical and functional analysis of HLA class II antigens induced by recombinant immune interferon on normal epidermal melanocytes. AU - Tsujisaki,M, AU - Igarashi,M, AU - Sakaguchi,K, AU - Eisinger,M, AU - Herlyn,M, AU - Ferrone,S, PY - 1987/2/15/pubmed PY - 1987/2/15/medline PY - 1987/2/15/entrez SP - 1310 EP - 6 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 138 IS - 4 N2 - The effect of recombinant immune interferon (IFN-gamma) on the expression and shedding of HLA antigens and of melanoma-associated antigens (MAA) by epidermal melanocytes was investigated by using serologic and immunochemical techniques. IFN-gamma enhances the expression and/or shedding of HLA class I antigens and of the cytoplasmic MAA defined by monoclonal antibody (MoAb) 465.12S and induces a slight reduction in the expression of the high m.w. melanoma-associated antigen (HMW-MAA). In agreement with the data in the literature, melanocytes incubated with IFN-gamma acquire HLA-DR, -DQ, and -DP antigens. Contrary to previous information in the literature, the effect is not restricted to HLA class II antigens, since IFN-gamma also induces the expression of the 96-kDa MAA recognized by MoAb CL203. The effect of IFN-gamma on HLA class II antigens and 96-kDa MAA is dose and time dependent and is specific, because recombinant leukocyte interferon affects the expression of neither type of antigen. In spite of the expression of HLA class II antigens, IFN-gamma-treated melanocytes do not acquire the ability to stimulate the proliferation of allogeneic lymphocytes. HLA-DR antigens are more susceptible to induction by IFN-gamma than HLA-DQ and -DP antigens, since the percentage of melanocytes acquiring HLA-DQ and -DP antigens is lower than that acquiring HLA-DR antigens. Furthermore, the dose of IFN-gamma is higher and the time of incubation is longer to induce HLA-DQ and -DP antigens than to induce HLA-DR antigens. The differential susceptibility of HLA-DR, -DQ, and -DP antigens as well as of melanocytes from various donors to the modulating effect of IFN-gamma may provide an explanation for the more frequent detection of HLA-DR than of HLA-DQ and -DP antigens in melanoma lesions and for the expression of HLA class II antigens by some, but not all, melanoma lesions. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/3100635/Immunochemical_and_functional_analysis_of_HLA_class_II_antigens_induced_by_recombinant_immune_interferon_on_normal_epidermal_melanocytes_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=3100635 DB - PRIME DP - Unbound Medicine ER -