Tags

Type your tag names separated by a space and hit enter

Electrospun polycaprolactone/hydroxyapatite/ZnO nanofibers as potential biomaterials for bone tissue regeneration.
J Mater Sci Mater Med 2019; 30(5):51JM

Abstract

Fabricating a bioartificial bone graft possessing structural, mechanical and biological properties mimicking the real bone matrix is a major challenge in bone tissue engineering. Moreover, the developed materials are prone to microbial invasion leading to biomaterial centered infections which might limit their clinical translation. In the present study, biomimetic nanofibrous scaffolds of Poly ɛ-caprolactone (PCL)/nano-hydroxyapatite (nHA) were electrospun with 1wt%, 5wt%, 10wt%, 15wt% and 30wt% of zinc oxide (ZnO) nanoparticles in order to understand the optimal concentration range of (ZnO) nanoparticles balancing both biocompatibility and osteoregeneration. The developed nanofibrous scaffolds were successfully characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDAX), contact angle, fourier transform infrared spectroscopy (FTIR), wide-angle X-Ray diffraction (WAXD), brunaueremmett Teller (BET) surface area and tensile testing. Biocompatibility of the developed scaffolds at in vitro level was evaluated by culturing MG-63 cells and investigating the impact on cell viability, proliferation, protein adsorption, alkaline phosphatase (ALP) activity and biomineralization. The PCL/nHA scaffolds exhibited a 1.2-fold increase in cell viability and proliferation, while incorporation of ZnO nanoparticles to PCL/nHA imparted antimicrobial activity to the scaffolds with a progressive increase in the antimicrobial efficacy with increasing ZnO concentration. The results of cell viability were supported by ALP activity and mineralization assay, wherein, PCL/nHA/ZnO scaffolds showed higher ALP activity and better mineralization capacity as compared to pristine PCL. Although, the PCL/nHA/ZnO scaffolds with 10, 15 and 30wt% of ZnO particles exhibited superior antimicrobial efficacy against both gram-negative (E. coli) and gram-positive (S. aureus) bacteria, a significant decrease in the cell viability and mechanical properties was observed at higher concentrations of ZnO namely 15 and 30%. Amongst the various ZnO concentrations studied optimal cell viability, antimicrobial effect and mechanical strength were observed at 10wt.% ZnO concentration. Thus, the present study revealed that the biomimetic tri-component PCL/nHA/ZnO scaffolds with ZnO concentration range of ≤ 10% could be ideal for achieving optimal biocompatibility (cell proliferation, biomineralization, and antimicrobial capacity) and mechanical stability thus making it a promising biomaterial substrate for bone tissue regeneration.

Authors+Show Affiliations

Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Gram- Lavale; Taluka- Mulshi, Pune, 412115, India.Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Gram- Lavale; Taluka- Mulshi, Pune, 412115, India.Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Gram- Lavale; Taluka- Mulshi, Pune, 412115, India. neetimohan27@gmail.com.Polymer Science and Engineering Division, CSIR-National Chemical Laboratory, Pune, 411008, India.School of Engineering, Ajeenkya DY Patil University (ADYPU), Pune, 412105, India.Polymer Science and Engineering Division, CSIR-National Chemical Laboratory, Pune, 411008, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31011810

Citation

Shitole, Ajinkya A., et al. "Electrospun polycaprolactone/hydroxyapatite/ZnO Nanofibers as Potential Biomaterials for Bone Tissue Regeneration." Journal of Materials Science. Materials in Medicine, vol. 30, no. 5, 2019, p. 51.
Shitole AA, Raut PW, Sharma N, et al. Electrospun polycaprolactone/hydroxyapatite/ZnO nanofibers as potential biomaterials for bone tissue regeneration. J Mater Sci Mater Med. 2019;30(5):51.
Shitole, A. A., Raut, P. W., Sharma, N., Giram, P., Khandwekar, A. P., & Garnaik, B. (2019). Electrospun polycaprolactone/hydroxyapatite/ZnO nanofibers as potential biomaterials for bone tissue regeneration. Journal of Materials Science. Materials in Medicine, 30(5), p. 51. doi:10.1007/s10856-019-6255-5.
Shitole AA, et al. Electrospun polycaprolactone/hydroxyapatite/ZnO Nanofibers as Potential Biomaterials for Bone Tissue Regeneration. J Mater Sci Mater Med. 2019 Apr 22;30(5):51. PubMed PMID: 31011810.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrospun polycaprolactone/hydroxyapatite/ZnO nanofibers as potential biomaterials for bone tissue regeneration. AU - Shitole,Ajinkya A, AU - Raut,Piyush W, AU - Sharma,Neeti, AU - Giram,Prabhanjan, AU - Khandwekar,Anand P, AU - Garnaik,Baijayantimala, Y1 - 2019/04/22/ PY - 2018/04/12/received PY - 2019/04/11/accepted PY - 2019/4/24/entrez PY - 2019/4/24/pubmed PY - 2019/12/4/medline SP - 51 EP - 51 JF - Journal of materials science. Materials in medicine JO - J Mater Sci Mater Med VL - 30 IS - 5 N2 - Fabricating a bioartificial bone graft possessing structural, mechanical and biological properties mimicking the real bone matrix is a major challenge in bone tissue engineering. Moreover, the developed materials are prone to microbial invasion leading to biomaterial centered infections which might limit their clinical translation. In the present study, biomimetic nanofibrous scaffolds of Poly ɛ-caprolactone (PCL)/nano-hydroxyapatite (nHA) were electrospun with 1wt%, 5wt%, 10wt%, 15wt% and 30wt% of zinc oxide (ZnO) nanoparticles in order to understand the optimal concentration range of (ZnO) nanoparticles balancing both biocompatibility and osteoregeneration. The developed nanofibrous scaffolds were successfully characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDAX), contact angle, fourier transform infrared spectroscopy (FTIR), wide-angle X-Ray diffraction (WAXD), brunaueremmett Teller (BET) surface area and tensile testing. Biocompatibility of the developed scaffolds at in vitro level was evaluated by culturing MG-63 cells and investigating the impact on cell viability, proliferation, protein adsorption, alkaline phosphatase (ALP) activity and biomineralization. The PCL/nHA scaffolds exhibited a 1.2-fold increase in cell viability and proliferation, while incorporation of ZnO nanoparticles to PCL/nHA imparted antimicrobial activity to the scaffolds with a progressive increase in the antimicrobial efficacy with increasing ZnO concentration. The results of cell viability were supported by ALP activity and mineralization assay, wherein, PCL/nHA/ZnO scaffolds showed higher ALP activity and better mineralization capacity as compared to pristine PCL. Although, the PCL/nHA/ZnO scaffolds with 10, 15 and 30wt% of ZnO particles exhibited superior antimicrobial efficacy against both gram-negative (E. coli) and gram-positive (S. aureus) bacteria, a significant decrease in the cell viability and mechanical properties was observed at higher concentrations of ZnO namely 15 and 30%. Amongst the various ZnO concentrations studied optimal cell viability, antimicrobial effect and mechanical strength were observed at 10wt.% ZnO concentration. Thus, the present study revealed that the biomimetic tri-component PCL/nHA/ZnO scaffolds with ZnO concentration range of ≤ 10% could be ideal for achieving optimal biocompatibility (cell proliferation, biomineralization, and antimicrobial capacity) and mechanical stability thus making it a promising biomaterial substrate for bone tissue regeneration. SN - 1573-4838 UR - https://www.unboundmedicine.com/medline/citation/31011810/Electrospun_polycaprolactone/hydroxyapatite/ZnO_nanofibers_as_potential_biomaterials_for_bone_tissue_regeneration_ L2 - https://doi.org/10.1007/s10856-019-6255-5 DB - PRIME DP - Unbound Medicine ER -