Tags

Type your tag names separated by a space and hit enter

Genotyping of long term persistent Staphylococcus aureus in bovine subclinical mastitis.
Microb Pathog. 2019 Jul; 132:45-50.MP

Abstract

Bovine mastitis affects dairy cattle worldwide and Staphylococcus aureus is one of the most common microorganisms involved in subclinical and chronic disease. Superantigens, such as enterotoxins contribute to S. aureus persistence and pathogenicity in this disease. Subclinical and chronic mastitis cases were diagnosed and S. aureus isolates from sub-clinical cases were investigated for carriage of virulence and antibiotic resistance genes that may contribute to long-term carriage and infection. Over a 12-month period, 116 S. aureus strains were recovered from 68 cows with subclinical mastitis. Classical enterotoxin genes (sea-see) were detected in 24.1% of isolates, and pvl and tsst-1 were identified in 3.4% and 46.6% the isolates, respectively. 18.1% that were persistent isolates were identified and characterized by pulsed field gel electrophoresis (PFGE), MLST, spa typing. Four isolates were methicillin-resistant S. aureus (MRSA) and belonged to SCCmec type I. Molecular typing showed that the agrI group was the most frequent, and a rare isolate was positive for both agrI and agrIII groups. Molecular characterization revealed the persistence of the spa type t10856 (ST133, clonal complex CC133, agr I), in a single animal for nine months and the persistence t605 (ST126, CC126) colonizing four animals for four months. These strains have been described recently in other herds in the same region, indicating their transmissibility and clonal expansion. We conclude that animals with subclinical mastitis are an important and somewhat overlooked reservoir for transmission within and between herds, and may carry virulence and antibiotic resistance genes contributing to persistent colonization, hinder the control of mastitis and may cause risks to the public health.

Authors+Show Affiliations

Department of Microbiology and Immunology, Institute of Biosciences, Sao Paulo State University, Botucatu, SP, Brazil.Department of Microbiology and Immunology, Institute of Biosciences, Sao Paulo State University, Botucatu, SP, Brazil.Department of Microbiology and Immunology, Institute of Biosciences, Sao Paulo State University, Botucatu, SP, Brazil.Department of Microbiology and Immunology, Institute of Biosciences, Sao Paulo State University, Botucatu, SP, Brazil.Department of Hygiene Veterinary and Public Health, Sao Paulo State University, Botucatu, SP, Brazil.Department of Hygiene Veterinary and Public Health, Sao Paulo State University, Botucatu, SP, Brazil.Department of Hygiene Veterinary and Public Health, Sao Paulo State University, Botucatu, SP, Brazil.Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland.Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland.Department of Microbiology and Immunology, Institute of Biosciences, Sao Paulo State University, Botucatu, SP, Brazil.Department of Microbiology and Immunology, Institute of Biosciences, Sao Paulo State University, Botucatu, SP, Brazil. Electronic address: vera.rall@unesp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31015015

Citation

Rossi, B F., et al. "Genotyping of Long Term Persistent Staphylococcus Aureus in Bovine Subclinical Mastitis." Microbial Pathogenesis, vol. 132, 2019, pp. 45-50.
Rossi BF, Bonsaglia ECR, Castilho IG, et al. Genotyping of long term persistent Staphylococcus aureus in bovine subclinical mastitis. Microb Pathog. 2019;132:45-50.
Rossi, B. F., Bonsaglia, E. C. R., Castilho, I. G., Dantas, S. T. A., Salina, A., Langoni, H., Pantoja, J. C. F., Budri, P. E., Fitzgerald-Hughes, D., Júnior, A. F., & Rall, V. L. M. (2019). Genotyping of long term persistent Staphylococcus aureus in bovine subclinical mastitis. Microbial Pathogenesis, 132, 45-50. https://doi.org/10.1016/j.micpath.2019.04.031
Rossi BF, et al. Genotyping of Long Term Persistent Staphylococcus Aureus in Bovine Subclinical Mastitis. Microb Pathog. 2019;132:45-50. PubMed PMID: 31015015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotyping of long term persistent Staphylococcus aureus in bovine subclinical mastitis. AU - Rossi,B F, AU - Bonsaglia,E C R, AU - Castilho,I G, AU - Dantas,S T A, AU - Salina,A, AU - Langoni,H, AU - Pantoja,J C F, AU - Budri,P E, AU - Fitzgerald-Hughes,D, AU - Júnior,A Fernandes, AU - Rall,V L M, Y1 - 2019/04/20/ PY - 2018/11/26/received PY - 2019/04/17/revised PY - 2019/04/18/accepted PY - 2019/4/25/pubmed PY - 2019/8/7/medline PY - 2019/4/25/entrez KW - PFGE KW - Persistence KW - agr KW - spa-type SP - 45 EP - 50 JF - Microbial pathogenesis JO - Microb Pathog VL - 132 N2 - Bovine mastitis affects dairy cattle worldwide and Staphylococcus aureus is one of the most common microorganisms involved in subclinical and chronic disease. Superantigens, such as enterotoxins contribute to S. aureus persistence and pathogenicity in this disease. Subclinical and chronic mastitis cases were diagnosed and S. aureus isolates from sub-clinical cases were investigated for carriage of virulence and antibiotic resistance genes that may contribute to long-term carriage and infection. Over a 12-month period, 116 S. aureus strains were recovered from 68 cows with subclinical mastitis. Classical enterotoxin genes (sea-see) were detected in 24.1% of isolates, and pvl and tsst-1 were identified in 3.4% and 46.6% the isolates, respectively. 18.1% that were persistent isolates were identified and characterized by pulsed field gel electrophoresis (PFGE), MLST, spa typing. Four isolates were methicillin-resistant S. aureus (MRSA) and belonged to SCCmec type I. Molecular typing showed that the agrI group was the most frequent, and a rare isolate was positive for both agrI and agrIII groups. Molecular characterization revealed the persistence of the spa type t10856 (ST133, clonal complex CC133, agr I), in a single animal for nine months and the persistence t605 (ST126, CC126) colonizing four animals for four months. These strains have been described recently in other herds in the same region, indicating their transmissibility and clonal expansion. We conclude that animals with subclinical mastitis are an important and somewhat overlooked reservoir for transmission within and between herds, and may carry virulence and antibiotic resistance genes contributing to persistent colonization, hinder the control of mastitis and may cause risks to the public health. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/31015015/Genotyping_of_long_term_persistent_Staphylococcus_aureus_in_bovine_subclinical_mastitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(18)32016-3 DB - PRIME DP - Unbound Medicine ER -