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Maximal exercise and erythrocyte fatty-acid status: a lipidomics study.
Physiol Rep. 2019 04; 7(8):e14040.PR

Abstract

Omega-3 fatty acids have long been ascribed a positive cardiovascular function. However, the plasma measurements invariably ignore 40% of the blood specimen, cells that engage in continuous exchange with their environment. In our study, we included all components of the circulating blood. Erythrocyte or red-blood-cell (RBC) n-3 fatty acid status has been linked to cardiovascular disease and death. A low omega-3 index is an independent risk factor for cardiovascular disease and mortality. We tested the hypothesis that acute, maximal exercise would influence the relationship between RBC and serum fatty acids. RBC fatty acids profiling was achieved using targeted HPLC-MS mass spectrometry. Healthy volunteers performed maximal treadmill exercise testing using the modified Bruce protocol. Central hemodynamics were monitored and maximal workload was assessed in metabolic equivalents (METs). Venous blood was obtained for RBC lipidomics. With the incremental exercise test, no fatty acid-level variations were found in RBCs, while heart rate and arterial blood pressure increased significantly. No changes occurred in the omega-3 quotient, namely the percentage of eicosapentaenoic acid and docosahexaenoic acid in RBC fatty acids in the RBC membrane. Nonetheless, maximal (13.50 ± 1.97 METs) exercise intensity led to a decrease of RBC lauric acid (C12:0) in the recovery period. These data suggest that despite significant hemodynamic effects, short-term maximal exercise is insufficient to alter RBC n-3 and other fatty-acid status, including the omega-3 quotient, in healthy individuals. RBC lauric acid deserves further scrutiny as a potential regulator of cardiovascular and metabolic functions.

Authors+Show Affiliations

Experimental and Clinical Research Center (ECRC), A Joint Institution of the Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, Berlin, Germany. HELIOS Klinikum Berlin-Buch, Berlin, Germany.LIPIDOMIX GmbH, Berlin, Germany.LIPIDOMIX GmbH, Berlin, Germany.Experimental and Clinical Research Center (ECRC), A Joint Institution of the Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, Berlin, Germany.Experimental and Clinical Research Center (ECRC), A Joint Institution of the Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, Berlin, Germany.

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31016868

Citation

Gollasch, Benjamin, et al. "Maximal Exercise and Erythrocyte Fatty-acid Status: a Lipidomics Study." Physiological Reports, vol. 7, no. 8, 2019, pp. e14040.
Gollasch B, Dogan I, Rothe M, et al. Maximal exercise and erythrocyte fatty-acid status: a lipidomics study. Physiol Rep. 2019;7(8):e14040.
Gollasch, B., Dogan, I., Rothe, M., Gollasch, M., & Luft, F. C. (2019). Maximal exercise and erythrocyte fatty-acid status: a lipidomics study. Physiological Reports, 7(8), e14040. https://doi.org/10.14814/phy2.14040
Gollasch B, et al. Maximal Exercise and Erythrocyte Fatty-acid Status: a Lipidomics Study. Physiol Rep. 2019;7(8):e14040. PubMed PMID: 31016868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maximal exercise and erythrocyte fatty-acid status: a lipidomics study. AU - Gollasch,Benjamin, AU - Dogan,Inci, AU - Rothe,Michael, AU - Gollasch,Maik, AU - Luft,Friedrich C, PY - 2019/01/30/received PY - 2019/03/05/revised PY - 2019/03/06/accepted PY - 2019/4/25/entrez PY - 2019/4/25/pubmed PY - 2020/5/1/medline KW - Erythrocytes KW - exercise KW - fatty acids KW - lipidomics SP - e14040 EP - e14040 JF - Physiological reports JO - Physiol Rep VL - 7 IS - 8 N2 - Omega-3 fatty acids have long been ascribed a positive cardiovascular function. However, the plasma measurements invariably ignore 40% of the blood specimen, cells that engage in continuous exchange with their environment. In our study, we included all components of the circulating blood. Erythrocyte or red-blood-cell (RBC) n-3 fatty acid status has been linked to cardiovascular disease and death. A low omega-3 index is an independent risk factor for cardiovascular disease and mortality. We tested the hypothesis that acute, maximal exercise would influence the relationship between RBC and serum fatty acids. RBC fatty acids profiling was achieved using targeted HPLC-MS mass spectrometry. Healthy volunteers performed maximal treadmill exercise testing using the modified Bruce protocol. Central hemodynamics were monitored and maximal workload was assessed in metabolic equivalents (METs). Venous blood was obtained for RBC lipidomics. With the incremental exercise test, no fatty acid-level variations were found in RBCs, while heart rate and arterial blood pressure increased significantly. No changes occurred in the omega-3 quotient, namely the percentage of eicosapentaenoic acid and docosahexaenoic acid in RBC fatty acids in the RBC membrane. Nonetheless, maximal (13.50 ± 1.97 METs) exercise intensity led to a decrease of RBC lauric acid (C12:0) in the recovery period. These data suggest that despite significant hemodynamic effects, short-term maximal exercise is insufficient to alter RBC n-3 and other fatty-acid status, including the omega-3 quotient, in healthy individuals. RBC lauric acid deserves further scrutiny as a potential regulator of cardiovascular and metabolic functions. SN - 2051-817X UR - https://www.unboundmedicine.com/medline/citation/31016868/Maximal_exercise_and_erythrocyte_fatty_acid_status:_a_lipidomics_study_ DB - PRIME DP - Unbound Medicine ER -