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Dietary Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA) Operate by Different Mechanisms to Modulate Hepatic Steatosis and Hyperinsulemia in fa/fa Zucker Rats.
Nutrients 2019; 11(4)N

Abstract

Hepatic steatosis, an early stage of non-alcoholic fatty liver disease, is commonly present in obesity and type 2 diabetes, and is associated with reduced hepatic omega-3 polyunsaturated fatty acid (n3-PUFA) status that impacts on the anti-inflammatory and insulin sensitizing functions of n3-PUFA. Our objective was to directly compare plant- and marine-based n3-PUFA (α-linoleic acid (ALA)), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA)) for their effects on hepatic steatosis, markers of hepatic inflammation and fibrosis, and insulinemia in obese rats. Fa/fa Zucker rats were provided diets containing ALA, EPA, DHA, or linoleic acid (LA, n6-PUFA) for eight weeks and compared to baseline fa/fa rats and lean Zucker rats fed LA-rich diet for eight weeks. Both DHA and EPA groups had liver lipid similar to baseline, however, DHA was more effective than EPA for reducing hepatic fatty acid synthase (FAS), increasing the proportion of smaller lipid droplets, reversing early fibrotic damage, and reducing fasting hyperinsulinemia. EPA was more effective for reducing FoxO1. Dietary ALA did not attenuate hepatic steatosis, most inflammatory markers or FAS. In summary, amongst the n3-PUFA, DHA was the most effective for elevating hepatic DHA levels, and preventing progression of hepatic steatosis via reductions in FAS and a marker of fibrosis.

Authors+Show Affiliations

Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. lenahong_@hotmail.com. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB R2H 2A6, Canada. lenahong_@hotmail.com.Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. pzahradka@sbrc.ca. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB R2H 2A6, Canada. pzahradka@sbrc.ca. Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, MB R3E 0J9, Canada. pzahradka@sbrc.ca.Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. lcordero-monroy@sbrc.ca. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB R2H 2A6, Canada. lcordero-monroy@sbrc.ca.Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB R2H 2A6, Canada. bwright@sbrc.ca.Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. ctaylor@sbrc.ca. Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB R2H 2A6, Canada. ctaylor@sbrc.ca. Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, MB R3E 0J9, Canada. ctaylor@sbrc.ca.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31022865

Citation

Hong, Lena, et al. "Dietary Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA) Operate By Different Mechanisms to Modulate Hepatic Steatosis and Hyperinsulemia in Fa/fa Zucker Rats." Nutrients, vol. 11, no. 4, 2019.
Hong L, Zahradka P, Cordero-Monroy L, et al. Dietary Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA) Operate by Different Mechanisms to Modulate Hepatic Steatosis and Hyperinsulemia in fa/fa Zucker Rats. Nutrients. 2019;11(4).
Hong, L., Zahradka, P., Cordero-Monroy, L., Wright, B., & Taylor, C. G. (2019). Dietary Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA) Operate by Different Mechanisms to Modulate Hepatic Steatosis and Hyperinsulemia in fa/fa Zucker Rats. Nutrients, 11(4), doi:10.3390/nu11040917.
Hong L, et al. Dietary Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA) Operate By Different Mechanisms to Modulate Hepatic Steatosis and Hyperinsulemia in Fa/fa Zucker Rats. Nutrients. 2019 Apr 24;11(4) PubMed PMID: 31022865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA) Operate by Different Mechanisms to Modulate Hepatic Steatosis and Hyperinsulemia in fa/fa Zucker Rats. AU - Hong,Lena, AU - Zahradka,Peter, AU - Cordero-Monroy,Luis, AU - Wright,Brenda, AU - Taylor,Carla G, Y1 - 2019/04/24/ PY - 2019/03/09/received PY - 2019/04/10/revised PY - 2019/04/19/accepted PY - 2019/4/27/entrez PY - 2019/4/27/pubmed PY - 2019/4/27/medline KW - docosahexaenoic acid KW - eicosapentaenoic acid KW - fa/fa Zucker rats KW - hepatic steatosis KW - inflammation KW - n3-fatty acids KW - α-linoleic acid JF - Nutrients JO - Nutrients VL - 11 IS - 4 N2 - Hepatic steatosis, an early stage of non-alcoholic fatty liver disease, is commonly present in obesity and type 2 diabetes, and is associated with reduced hepatic omega-3 polyunsaturated fatty acid (n3-PUFA) status that impacts on the anti-inflammatory and insulin sensitizing functions of n3-PUFA. Our objective was to directly compare plant- and marine-based n3-PUFA (α-linoleic acid (ALA)), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA)) for their effects on hepatic steatosis, markers of hepatic inflammation and fibrosis, and insulinemia in obese rats. Fa/fa Zucker rats were provided diets containing ALA, EPA, DHA, or linoleic acid (LA, n6-PUFA) for eight weeks and compared to baseline fa/fa rats and lean Zucker rats fed LA-rich diet for eight weeks. Both DHA and EPA groups had liver lipid similar to baseline, however, DHA was more effective than EPA for reducing hepatic fatty acid synthase (FAS), increasing the proportion of smaller lipid droplets, reversing early fibrotic damage, and reducing fasting hyperinsulinemia. EPA was more effective for reducing FoxO1. Dietary ALA did not attenuate hepatic steatosis, most inflammatory markers or FAS. In summary, amongst the n3-PUFA, DHA was the most effective for elevating hepatic DHA levels, and preventing progression of hepatic steatosis via reductions in FAS and a marker of fibrosis. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/31022865/Dietary_Docosahexaenoic_Acid_(DHA)_and_Eicosapentaenoic_Acid_(EPA)_Operate_by_Different_Mechanisms_to_Modulate_Hepatic_Steatosis_and_Hyperinsulemia_in_fa/fa_Zucker_Rats L2 - http://www.mdpi.com/resolver?pii=nu11040917 DB - PRIME DP - Unbound Medicine ER -