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Characterization of a New Member of Alphacoronavirus with Unique Genomic Features in Rhinolophus Bats.
Viruses. 2019 04 24; 11(4)V

Abstract

Bats have been identified as a natural reservoir of a variety of coronaviruses (CoVs). Several of them have caused diseases in humans and domestic animals by interspecies transmission. Considering the diversity of bat coronaviruses, bat species and populations, we expect to discover more bat CoVs through virus surveillance. In this study, we described a new member of alphaCoV (BtCoV/Rh/YN2012) in bats with unique genome features. Unique accessory genes, ORF4a and ORF4b were found between the spike gene and the envelope gene, while ORF8 gene was found downstream of the nucleocapsid gene. All the putative genes were further confirmed by reverse-transcription analyses. One unique gene at the 3' end of the BtCoV/Rh/YN2012 genome, ORF9, exhibits ~30% amino acid identity to ORF7a of the SARS-related coronavirus. Functional analysis showed ORF4a protein can activate IFN-β production, whereas ORF3a can regulate NF-κB production. We also screened the spike-mediated virus entry using the spike-pseudotyped retroviruses system, although failed to find any fully permissive cells. Our results expand the knowledge on the genetic diversity of bat coronaviruses. Continuous screening of bat viruses will help us further understand the important role played by bats in coronavirus evolution and transmission.

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Authors+Show Affiliations

Wang N
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. wuzhongxiaoliu@163.com. College of Life Science, University of Chinese Academy of Sciences, Beijing 100864, China. wuzhongxiaoliu@163.com.
Luo C
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. chine.lcm@163.com. College of Life Science, University of Chinese Academy of Sciences, Beijing 100864, China. chine.lcm@163.com.
Liu H
Center for Emerging Infectious Disease, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. liuhz@wh.iov.cn.
Yang X
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. yangxl@wh.iov.cn.
Hu B
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. ouihaagendazs@hotmail.com.
Zhang W
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. happyzhang_2007@163.com.
Li B
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. shenqi0622@126.com.
Zhu Y
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. zhuyan@wh.iov.cn.
Zhu G
EcoHealth Alliance, New York, NY 10001, USA. zhugj609@126.com.
Shen X
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. pukulovesu@163.com. College of Life Science, University of Chinese Academy of Sciences, Beijing 100864, China. pukulovesu@163.com.
Peng C
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. pengcheng@wh.iov.cn.
Shi Z
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. zlshi@wh.iov.cn.

MeSH

AlphacoronavirusAnimalsChinaChiropteraCoronavirus InfectionsEvolution, MolecularGenetic VariationGenome, ViralHigh-Throughput Nucleotide SequencingOpen Reading FramesPhylogenySequence Analysis, DNAViral Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

31022925

Citation

Wang, Ning, et al. "Characterization of a New Member of Alphacoronavirus With Unique Genomic Features in Rhinolophus Bats." Viruses, vol. 11, no. 4, 2019.
Wang N, Luo C, Liu H, et al. Characterization of a New Member of Alphacoronavirus with Unique Genomic Features in Rhinolophus Bats. Viruses. 2019;11(4).
Wang, N., Luo, C., Liu, H., Yang, X., Hu, B., Zhang, W., Li, B., Zhu, Y., Zhu, G., Shen, X., Peng, C., & Shi, Z. (2019). Characterization of a New Member of Alphacoronavirus with Unique Genomic Features in Rhinolophus Bats. Viruses, 11(4). https://doi.org/10.3390/v11040379
Wang N, et al. Characterization of a New Member of Alphacoronavirus With Unique Genomic Features in Rhinolophus Bats. Viruses. 2019 04 24;11(4) PubMed PMID: 31022925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a New Member of Alphacoronavirus with Unique Genomic Features in Rhinolophus Bats. AU - Wang,Ning, AU - Luo,Chuming, AU - Liu,Haizhou, AU - Yang,Xinglou, AU - Hu,Ben, AU - Zhang,Wei, AU - Li,Bei, AU - Zhu,Yan, AU - Zhu,Guangjian, AU - Shen,Xurui, AU - Peng,Cheng, AU - Shi,Zhengli, Y1 - 2019/04/24/ PY - 2019/03/13/received PY - 2019/04/14/revised PY - 2019/04/22/accepted PY - 2019/4/27/entrez PY - 2019/4/27/pubmed PY - 2020/2/20/medline KW - Rhinolophus bat KW - alphacoronavirus KW - coronavirus KW - unique genes JF - Viruses JO - Viruses VL - 11 IS - 4 N2 - Bats have been identified as a natural reservoir of a variety of coronaviruses (CoVs). Several of them have caused diseases in humans and domestic animals by interspecies transmission. Considering the diversity of bat coronaviruses, bat species and populations, we expect to discover more bat CoVs through virus surveillance. In this study, we described a new member of alphaCoV (BtCoV/Rh/YN2012) in bats with unique genome features. Unique accessory genes, ORF4a and ORF4b were found between the spike gene and the envelope gene, while ORF8 gene was found downstream of the nucleocapsid gene. All the putative genes were further confirmed by reverse-transcription analyses. One unique gene at the 3' end of the BtCoV/Rh/YN2012 genome, ORF9, exhibits ~30% amino acid identity to ORF7a of the SARS-related coronavirus. Functional analysis showed ORF4a protein can activate IFN-β production, whereas ORF3a can regulate NF-κB production. We also screened the spike-mediated virus entry using the spike-pseudotyped retroviruses system, although failed to find any fully permissive cells. Our results expand the knowledge on the genetic diversity of bat coronaviruses. Continuous screening of bat viruses will help us further understand the important role played by bats in coronavirus evolution and transmission. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/31022925/Characterization_of_a_New_Member_of_Alphacoronavirus_with_Unique_Genomic_Features_in_Rhinolophus_Bats_ DB - PRIME DP - Unbound Medicine ER -