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New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds.
Int J Mol Sci. 2019 Apr 25; 20(8)IJ

Abstract

Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking. Two oligo probes, Oligo-B11 and Oligo-pThp3.93, were designed according to the published Thinopyrum ponticum (Th. ponticum)-specific repetitive sequences. Both Oligo-B11 and Oligo-pThp3.93 can be used for ND-FISH analysis and can replace conventional GISH and FISH to discriminate some chromosomes of Th. elongatum, Th. intermedium, and Th. ponticum in wheat backgrounds. The two oligo probes provide a convenient way for the utilization of Thinopyrum germplasms in future wheat breeding programs.

Authors+Show Affiliations

Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. xiwei923700915@163.com. College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. xiwei923700915@163.com.Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. zxtang@sicau.edu.cn. College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. zxtang@sicau.edu.cn.Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. tangshuyao705708@sina.com. College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. tangshuyao705708@sina.com.Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China. yangzujun@uestc.edu.cn.Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. luojie2010@sina.com. College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. luojie2010@sina.com.Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. fushulan@sicau.edu.cn. College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. fushulan@sicau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31027154

Citation

Xi, Wei, et al. "New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds." International Journal of Molecular Sciences, vol. 20, no. 8, 2019.
Xi W, Tang Z, Tang S, et al. New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds. Int J Mol Sci. 2019;20(8).
Xi, W., Tang, Z., Tang, S., Yang, Z., Luo, J., & Fu, S. (2019). New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds. International Journal of Molecular Sciences, 20(8). https://doi.org/10.3390/ijms20082031
Xi W, et al. New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds. Int J Mol Sci. 2019 Apr 25;20(8) PubMed PMID: 31027154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds. AU - Xi,Wei, AU - Tang,Zongxiang, AU - Tang,Shuyao, AU - Yang,Zujun, AU - Luo,Jie, AU - Fu,Shulan, Y1 - 2019/04/25/ PY - 2019/04/04/received PY - 2019/04/20/revised PY - 2019/04/22/accepted PY - 2019/4/28/entrez PY - 2019/4/28/pubmed PY - 2019/8/14/medline KW - ND-FISH KW - Thinopyrum KW - chromosome KW - oligo probe KW - wheat JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 8 N2 - Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking. Two oligo probes, Oligo-B11 and Oligo-pThp3.93, were designed according to the published Thinopyrum ponticum (Th. ponticum)-specific repetitive sequences. Both Oligo-B11 and Oligo-pThp3.93 can be used for ND-FISH analysis and can replace conventional GISH and FISH to discriminate some chromosomes of Th. elongatum, Th. intermedium, and Th. ponticum in wheat backgrounds. The two oligo probes provide a convenient way for the utilization of Thinopyrum germplasms in future wheat breeding programs. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31027154/New_ND_FISH_Positive_Oligo_Probes_for_Identifying_Thinopyrum_Chromosomes_in_Wheat_Backgrounds_ L2 - https://www.mdpi.com/resolver?pii=ijms20082031 DB - PRIME DP - Unbound Medicine ER -