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Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media.
Sci Data 2019; 6(1):43SD

Abstract

Cation adjusted-Mueller Hinton Broth (CA-MHB) is the standard bacteriological medium utilized in the clinic for the determination of antibiotic susceptibility. However, a growing number of literature has demonstrated that media conditions can cause a substantial difference in the efficacy of antibiotics and antimicrobials. Recent studies have also shown that minimum inhibitory concentration (MIC) tests performed in standard cell culture media (e.g. RPMI and DMEM) are more indicative of in vivo antibiotic efficacy, presumably because they are a better proxy for the human host's physiological conditions. The basis for the bacterial media dependent susceptibility to antibiotics remains undefined. To address this question, we characterized the physiological response of methicillin-resistant Staphylococcus aureus (MRSA) during exposure to sub-inhibitory concentrations of the beta-lactam antibiotic nafcillin in either CA-MHB or RPMI + 10% LB (R10LB). Here, we present high quality transcriptomic, exo-metabolomic and morphological data paired with growth and susceptibility results for MRSA cultured in either standard bacteriologic or more physiologic relevant medium.

Authors+Show Affiliations

Department of Bioengineering, University of California, San Diego, La Jolla, USA.Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, USA. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA.Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA.Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA.Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA.Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA.Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, USA. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA. Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, 92093, USA. Center for Microbiome Innovation, University of California San Diego, La Jolla, CA, 92093, USA.Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA. Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA. Department of Computer Science and Engineering, University of California San Diego, La Jolla, CA, 92093, USA. Center for Microbiome Innovation, University of California San Diego, La Jolla, CA, 92093, USA.Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA. Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA. Center for Microbiome Innovation, University of California San Diego, La Jolla, CA, 92093, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA. Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800, Kongens, Lyngby, Denmark. Center for Microbiome Innovation, University of California San Diego, La Jolla, CA, 92093, USA.Department of Bioengineering, University of California, San Diego, La Jolla, USA. afeist@ucsd.edu. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800, Kongens, Lyngby, Denmark. afeist@ucsd.edu.

Pub Type(s)

Dataset
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

31028276

Citation

Poudel, Saugat, et al. "Characterization of CA-MRSA TCH1516 Exposed to Nafcillin in Bacteriological and Physiological Media." Scientific Data, vol. 6, no. 1, 2019, p. 43.
Poudel S, Tsunemoto H, Meehan M, et al. Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media. Sci Data. 2019;6(1):43.
Poudel, S., Tsunemoto, H., Meehan, M., Szubin, R., Olson, C. A., Lamsa, A., ... Feist, A. M. (2019). Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media. Scientific Data, 6(1), p. 43. doi:10.1038/s41597-019-0051-4.
Poudel S, et al. Characterization of CA-MRSA TCH1516 Exposed to Nafcillin in Bacteriological and Physiological Media. Sci Data. 2019 04 26;6(1):43. PubMed PMID: 31028276.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of CA-MRSA TCH1516 exposed to nafcillin in bacteriological and physiological media. AU - Poudel,Saugat, AU - Tsunemoto,Hannah, AU - Meehan,Michael, AU - Szubin,Richard, AU - Olson,Connor A, AU - Lamsa,Anne, AU - Seif,Yara, AU - Dillon,Nicholas, AU - Vrbanac,Alison, AU - Sugie,Joseph, AU - Dahesh,Samira, AU - Monk,Jonathan M, AU - Dorrestein,Pieter C, AU - Pogliano,Joseph, AU - Knight,Rob, AU - Nizet,Victor, AU - Palsson,Bernhard O, AU - Feist,Adam M, Y1 - 2019/04/26/ PY - 2018/11/16/received PY - 2019/03/07/accepted PY - 2019/4/28/entrez PY - 2019/4/28/pubmed PY - 2019/5/15/medline SP - 43 EP - 43 JF - Scientific data JO - Sci Data VL - 6 IS - 1 N2 - Cation adjusted-Mueller Hinton Broth (CA-MHB) is the standard bacteriological medium utilized in the clinic for the determination of antibiotic susceptibility. However, a growing number of literature has demonstrated that media conditions can cause a substantial difference in the efficacy of antibiotics and antimicrobials. Recent studies have also shown that minimum inhibitory concentration (MIC) tests performed in standard cell culture media (e.g. RPMI and DMEM) are more indicative of in vivo antibiotic efficacy, presumably because they are a better proxy for the human host's physiological conditions. The basis for the bacterial media dependent susceptibility to antibiotics remains undefined. To address this question, we characterized the physiological response of methicillin-resistant Staphylococcus aureus (MRSA) during exposure to sub-inhibitory concentrations of the beta-lactam antibiotic nafcillin in either CA-MHB or RPMI + 10% LB (R10LB). Here, we present high quality transcriptomic, exo-metabolomic and morphological data paired with growth and susceptibility results for MRSA cultured in either standard bacteriologic or more physiologic relevant medium. SN - 2052-4463 UR - https://www.unboundmedicine.com/medline/citation/31028276/Characterization_of_CA-MRSA_TCH1516_exposed_to_nafcillin_in_bacteriological_and_physiological_media L2 - http://dx.doi.org/10.1038/s41597-019-0051-4 DB - PRIME DP - Unbound Medicine ER -