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Offline preparative 2-D polar-copolymerized reversed-phase chromatography × zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori.

Abstract

Polar compounds are abundant in the water decoction of Traditional Chinese Medicines (TCMs) and possess important biological activities. However, purifying these compounds has faced great difficulties largely due to poor retention and insufficient selectivity. To solve this problem, an offline orthogonal 2-D RPLC coupled with hydrophilic interaction chromatography (HILIC) method was developed to achieve purification of polar compounds from Caulis Polygoni Multiflori. A polar-copolymerized XAqua C18 column and a zwitterionic Click XIon column exhibited satisfactory retention and separation for polar compounds. Therefore, they were adopted to construct the offline 2-D LC system. Furthermore, the method presented a high orthogonality, which was calculated to be 72%. The XAqua C18 column was used in the first dimension to fractionate the 5.1 g polar fraction with a recovery of 85% within 10 h. In the second dimension, three representative fractions were purified using the Click XIon column. Finally, three compounds with purity higher than 95% were identified for the first time from this plant. This offline 2-D RPLC/HILIC method was shown to be an effective approach to purifying polar compounds from Caulis Polygoni Multiflori.

Authors+Show Affiliations

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China; University of Chinese Academy of Sciences, Beijing, China.Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China. Electronic address: liuyanfang@dicp.ac.cn.Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China. Electronic address: liangxm@dicp.ac.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31029036

Citation

Liu, Dian, et al. "Offline Preparative 2-D Polar-copolymerized Reversed-phase Chromatography × Zwitterionic Hydrophilic Interaction Chromatography for Effective Purification of Polar Compounds From Caulis Polygoni Multiflori." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1118-1119, 2019, pp. 70-77.
Liu D, Jin H, Wang J, et al. Offline preparative 2-D polar-copolymerized reversed-phase chromatography × zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori. J Chromatogr B Analyt Technol Biomed Life Sci. 2019;1118-1119:70-77.
Liu, D., Jin, H., Wang, J., Zhou, H., Liu, Y., Feng, J., & Liang, X. (2019). Offline preparative 2-D polar-copolymerized reversed-phase chromatography × zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1118-1119, pp. 70-77. doi:10.1016/j.jchromb.2019.04.039.
Liu D, et al. Offline Preparative 2-D Polar-copolymerized Reversed-phase Chromatography × Zwitterionic Hydrophilic Interaction Chromatography for Effective Purification of Polar Compounds From Caulis Polygoni Multiflori. J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Jun 15;1118-1119:70-77. PubMed PMID: 31029036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Offline preparative 2-D polar-copolymerized reversed-phase chromatography × zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori. AU - Liu,Dian, AU - Jin,Hongli, AU - Wang,Jixia, AU - Zhou,Han, AU - Liu,Yanfang, AU - Feng,Jiatao, AU - Liang,Xinmiao, Y1 - 2019/04/18/ PY - 2019/01/03/received PY - 2019/04/17/revised PY - 2019/04/17/accepted PY - 2019/4/28/pubmed PY - 2019/6/18/medline PY - 2019/4/28/entrez KW - Caulis Polygoni Multiflori KW - Polar compounds KW - Polar-copolymerized reversed-phase chromatography KW - Zwitterionic hydrophilic interaction chromatography SP - 70 EP - 77 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 1118-1119 N2 - Polar compounds are abundant in the water decoction of Traditional Chinese Medicines (TCMs) and possess important biological activities. However, purifying these compounds has faced great difficulties largely due to poor retention and insufficient selectivity. To solve this problem, an offline orthogonal 2-D RPLC coupled with hydrophilic interaction chromatography (HILIC) method was developed to achieve purification of polar compounds from Caulis Polygoni Multiflori. A polar-copolymerized XAqua C18 column and a zwitterionic Click XIon column exhibited satisfactory retention and separation for polar compounds. Therefore, they were adopted to construct the offline 2-D LC system. Furthermore, the method presented a high orthogonality, which was calculated to be 72%. The XAqua C18 column was used in the first dimension to fractionate the 5.1 g polar fraction with a recovery of 85% within 10 h. In the second dimension, three representative fractions were purified using the Click XIon column. Finally, three compounds with purity higher than 95% were identified for the first time from this plant. This offline 2-D RPLC/HILIC method was shown to be an effective approach to purifying polar compounds from Caulis Polygoni Multiflori. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/31029036/Offline_preparative_2_D_polar_copolymerized_reversed_phase_chromatography_×_zwitterionic_hydrophilic_interaction_chromatography_for_effective_purification_of_polar_compounds_from_Caulis_Polygoni_Multiflori_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(19)30012-1 DB - PRIME DP - Unbound Medicine ER -