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Bi-layered α-tocopherol acetate loaded membranes for potential wound healing and skin regeneration.
Mater Sci Eng C Mater Biol Appl. 2019 Aug; 101:438-447.MS

Abstract

With an increase in the demand for skin regeneration products, there is a noticeable increase in developing materials that encourage, wound healing and skin regeneration. It has been reported that antioxidants play an important role in anti-inflammatory reactions, cellular proliferation and remodeling phase of wound healing. While consideration all these factors, a novel α-tocopherol acetate (vitamin E) (VE) loaded bi-layered electrospun membrane, based on lower polycaprolactone (PCL) layer and upper polylactic acid (PLA) layer, was fabricated through electrospinning. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), in-vitro degradation studies, swelling studies and VE release studies were performed to evaluate structural, physical and in-vitro behavior of membranes. Biological properties of membranes were evaluated through cell proliferation assay, cell adhesion studies, live/dead cell assay and CAM assay. SEM images showed that the average diameter of nanofibers ranged from 1 to 6 μm, while addition of VE changed the diameter and morphology of fibers. Bi-layered membranes showed significant swelling behavior through water uptake, membranes loaded with 30% VE showed 8.7% and 6.8% degradation in lysozyme and H2O2 respectively. 20% and 30% VE loaded membranes followed Korsmeyer-Peppas and first order drug release kinetics followed by non-fickian drug release kinetics. Membranes showed non-toxic behavior and supported cell proliferation via alamar blue assay, cell adhesion via SEM, cell viability via live/dead assay and wound healing by scratch assay. CAM assay showed that membranes having VE supported angiogenesis and showed significant formation of blood vessels making it suitable for skin regeneration and wound healing. Results showed that large surface area of nanofibers, porous structure and biocompatible nature are suitable for targeted clinical applications.

Authors+Show Affiliations

Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan.Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan. Electronic address: hamadkhalid@cuilahore.edu.pk.Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan.Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan.Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan.Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan.Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan.Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan.Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan.Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan.Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Defence Road, Off Raiwind Road, Lahore 54000, Pakistan. Electronic address: atherfarooq@cuilahore.edu.pk.Department of Engineering, New Engineering Building, Lancaster University, Gillow Ave, Bailrigg, Lancaster LA1 4YW, UK.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31029339

Citation

Zahid, Saba, et al. "Bi-layered Α-tocopherol Acetate Loaded Membranes for Potential Wound Healing and Skin Regeneration." Materials Science & Engineering. C, Materials for Biological Applications, vol. 101, 2019, pp. 438-447.
Zahid S, Khalid H, Ikram F, et al. Bi-layered α-tocopherol acetate loaded membranes for potential wound healing and skin regeneration. Mater Sci Eng C Mater Biol Appl. 2019;101:438-447.
Zahid, S., Khalid, H., Ikram, F., Iqbal, H., Samie, M., Shahzadi, L., Shah, A. T., Yar, M., Chaudhry, A. A., Awan, S. J., Khan, A. F., & Rehman, I. U. (2019). Bi-layered α-tocopherol acetate loaded membranes for potential wound healing and skin regeneration. Materials Science & Engineering. C, Materials for Biological Applications, 101, 438-447. https://doi.org/10.1016/j.msec.2019.03.080
Zahid S, et al. Bi-layered Α-tocopherol Acetate Loaded Membranes for Potential Wound Healing and Skin Regeneration. Mater Sci Eng C Mater Biol Appl. 2019;101:438-447. PubMed PMID: 31029339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bi-layered α-tocopherol acetate loaded membranes for potential wound healing and skin regeneration. AU - Zahid,Saba, AU - Khalid,Hamad, AU - Ikram,Fakhera, AU - Iqbal,Haffsah, AU - Samie,Muhammad, AU - Shahzadi,Lubna, AU - Shah,Asma Tufail, AU - Yar,Muhammad, AU - Chaudhry,Aqif Anwar, AU - Awan,Sana Javaid, AU - Khan,Ather Farooq, AU - Rehman,Ihtesham Ur, Y1 - 2019/03/23/ PY - 2018/12/20/received PY - 2019/03/19/revised PY - 2019/03/22/accepted PY - 2019/4/29/entrez PY - 2019/4/29/pubmed PY - 2019/8/20/medline KW - Angiogenesis KW - PCL/PLA membranes KW - Vitamin E KW - Wound healing SP - 438 EP - 447 JF - Materials science & engineering. C, Materials for biological applications JO - Mater Sci Eng C Mater Biol Appl VL - 101 N2 - With an increase in the demand for skin regeneration products, there is a noticeable increase in developing materials that encourage, wound healing and skin regeneration. It has been reported that antioxidants play an important role in anti-inflammatory reactions, cellular proliferation and remodeling phase of wound healing. While consideration all these factors, a novel α-tocopherol acetate (vitamin E) (VE) loaded bi-layered electrospun membrane, based on lower polycaprolactone (PCL) layer and upper polylactic acid (PLA) layer, was fabricated through electrospinning. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), in-vitro degradation studies, swelling studies and VE release studies were performed to evaluate structural, physical and in-vitro behavior of membranes. Biological properties of membranes were evaluated through cell proliferation assay, cell adhesion studies, live/dead cell assay and CAM assay. SEM images showed that the average diameter of nanofibers ranged from 1 to 6 μm, while addition of VE changed the diameter and morphology of fibers. Bi-layered membranes showed significant swelling behavior through water uptake, membranes loaded with 30% VE showed 8.7% and 6.8% degradation in lysozyme and H2O2 respectively. 20% and 30% VE loaded membranes followed Korsmeyer-Peppas and first order drug release kinetics followed by non-fickian drug release kinetics. Membranes showed non-toxic behavior and supported cell proliferation via alamar blue assay, cell adhesion via SEM, cell viability via live/dead assay and wound healing by scratch assay. CAM assay showed that membranes having VE supported angiogenesis and showed significant formation of blood vessels making it suitable for skin regeneration and wound healing. Results showed that large surface area of nanofibers, porous structure and biocompatible nature are suitable for targeted clinical applications. SN - 1873-0191 UR - https://www.unboundmedicine.com/medline/citation/31029339/Bi_layered_α_tocopherol_acetate_loaded_membranes_for_potential_wound_healing_and_skin_regeneration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-4931(18)33902-X DB - PRIME DP - Unbound Medicine ER -