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An aptamer based aggregation assay for the neonicotinoid insecticide acetamiprid using fluorescent upconversion nanoparticles and DNA functionalized gold nanoparticles.
Mikrochim Acta. 2019 04 27; 186(5):308.MA

Abstract

An acetamiprid-binding aptamer (ABA), gold nanoparticles (AuNPs) and upconversion nanoparticles (UCNPs) are used in a colorimetric and fluorometric method for the ultrasensitive and selective detection of the pesticide acetamiprid. The ABA is first configured into a duplex with a complementary DNA covalently attached to AuNPs. The resulting dsDNA-functionalized AuNP probe is not stable in 0.15 M NaCl solution and aggregates. This causing the color to change from red to purple. In the presence of acetamiprid, the ABA undergoes a structural switch from a DNA duplex to an aptamer-acetamiprid complex and consequently dissociates from the AuNPs. The partially unhybridized AuNPs are stable against salt-induced aggregation and show red color. The ratio of absorbances at 524 nm (red) and 650 nm (purple blue) varies with the concentration of acetamiprid in the 0.025-10 μM concentration range. The colorimetric signal can be further amplified by introducing DNA-modified carboxylated UCNPs (silica-coated NaYF4:Yb,Er) which display red and green fluorescence under 980 nm excitation. An inner filter effect occurs between DNA-modified UCNPs and dsDNA-modified AuNPs. The fluorometric assay is based on the measurement of the ratio of red (654 nm) and green (540 nm) fluorescence and works in the 0.025 to 1 μM acetamiprid concentration range and has a 0.36 nM detection limit (at a signal-to-noise ratio of 3). Because of the specificity of the aptamer, the assay is high selective. It was successfully used to quantify acetamiprid in contaminated real samples. Graphical abstract Schematic presentation of an upconversion fluorescent assay for acetamiprid. It involves the principle of analyte-triggered structural switch of aptamers, salt-induced AuNP aggregation, and signal amplification from UCNP.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yang L
State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong, 266580, People's Republic of China.
Sun H
State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong, 266580, People's Republic of China.
Wang X
State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong, 266580, People's Republic of China.
Yao W
Center for Evidence Identification, Chongqing Public Security Bureau, Chongqing, 401147, People's Republic of China.
Zhang W
State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong, 266580, People's Republic of China.
Jiang L
State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong, 266580, People's Republic of China. leijiang@upc.edu.cn.

MeSH

AnimalsAptamers, NucleotideBase SequenceBiosensing TechniquesDNAFluorometryGoldInsecticidesMetal NanoparticlesModels, MolecularNeonicotinoidsNucleic Acid Conformation

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31030275

Citation

Yang, Limin, et al. "An Aptamer Based Aggregation Assay for the Neonicotinoid Insecticide Acetamiprid Using Fluorescent Upconversion Nanoparticles and DNA Functionalized Gold Nanoparticles." Mikrochimica Acta, vol. 186, no. 5, 2019, p. 308.
Yang L, Sun H, Wang X, et al. An aptamer based aggregation assay for the neonicotinoid insecticide acetamiprid using fluorescent upconversion nanoparticles and DNA functionalized gold nanoparticles. Mikrochim Acta. 2019;186(5):308.
Yang, L., Sun, H., Wang, X., Yao, W., Zhang, W., & Jiang, L. (2019). An aptamer based aggregation assay for the neonicotinoid insecticide acetamiprid using fluorescent upconversion nanoparticles and DNA functionalized gold nanoparticles. Mikrochimica Acta, 186(5), 308. https://doi.org/10.1007/s00604-019-3422-9
Yang L, et al. An Aptamer Based Aggregation Assay for the Neonicotinoid Insecticide Acetamiprid Using Fluorescent Upconversion Nanoparticles and DNA Functionalized Gold Nanoparticles. Mikrochim Acta. 2019 04 27;186(5):308. PubMed PMID: 31030275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An aptamer based aggregation assay for the neonicotinoid insecticide acetamiprid using fluorescent upconversion nanoparticles and DNA functionalized gold nanoparticles. AU - Yang,Limin, AU - Sun,Haifeng, AU - Wang,Xuan, AU - Yao,Weijing, AU - Zhang,Wenjuan, AU - Jiang,Lei, Y1 - 2019/04/27/ PY - 2018/11/30/received PY - 2019/04/08/accepted PY - 2019/4/29/entrez PY - 2019/4/29/pubmed PY - 2020/3/5/medline KW - Colloidal stability KW - Colorimetric assay KW - Dual readout KW - Gold nanoparticles KW - Inner filter effect KW - Upconversion fluorescence KW - Upconversion nanoparticles SP - 308 EP - 308 JF - Mikrochimica acta JO - Mikrochim Acta VL - 186 IS - 5 N2 - An acetamiprid-binding aptamer (ABA), gold nanoparticles (AuNPs) and upconversion nanoparticles (UCNPs) are used in a colorimetric and fluorometric method for the ultrasensitive and selective detection of the pesticide acetamiprid. The ABA is first configured into a duplex with a complementary DNA covalently attached to AuNPs. The resulting dsDNA-functionalized AuNP probe is not stable in 0.15 M NaCl solution and aggregates. This causing the color to change from red to purple. In the presence of acetamiprid, the ABA undergoes a structural switch from a DNA duplex to an aptamer-acetamiprid complex and consequently dissociates from the AuNPs. The partially unhybridized AuNPs are stable against salt-induced aggregation and show red color. The ratio of absorbances at 524 nm (red) and 650 nm (purple blue) varies with the concentration of acetamiprid in the 0.025-10 μM concentration range. The colorimetric signal can be further amplified by introducing DNA-modified carboxylated UCNPs (silica-coated NaYF4:Yb,Er) which display red and green fluorescence under 980 nm excitation. An inner filter effect occurs between DNA-modified UCNPs and dsDNA-modified AuNPs. The fluorometric assay is based on the measurement of the ratio of red (654 nm) and green (540 nm) fluorescence and works in the 0.025 to 1 μM acetamiprid concentration range and has a 0.36 nM detection limit (at a signal-to-noise ratio of 3). Because of the specificity of the aptamer, the assay is high selective. It was successfully used to quantify acetamiprid in contaminated real samples. Graphical abstract Schematic presentation of an upconversion fluorescent assay for acetamiprid. It involves the principle of analyte-triggered structural switch of aptamers, salt-induced AuNP aggregation, and signal amplification from UCNP. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/31030275/An_aptamer_based_aggregation_assay_for_the_neonicotinoid_insecticide_acetamiprid_using_fluorescent_upconversion_nanoparticles_and_DNA_functionalized_gold_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -